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Reactive oxygen species are involved in nitric oxide-induced apoptosis in rat cortical neurons

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Abstract

Nitric oxide donor SNAP induced apoptosis in primary rat cerebral cortical neurons, which was characterized morphologically by chromatin condensation and the formation of apoptotic bodies. With redox-sensitive fluorescence probes DCFH-DA and DHR123, the formation of endogenous reactive oxygen species (ROS) inside cells during the apoptosis process was monitored by laser confocal scanning microscopy (LCSM). SNAP treatment also caused the accumulation of extracellular hydrogen peroxide. Pretreatment with the nitric oxide scavenger hemoglobin could effectively inhibit the formation of endogenous ROS and protect neurons from apoptosis. The results suggested that ROS might be involved in NO-induced apoptosis in neuronal cells.

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References

  1. R.D. Hawkins, Neuron 16, 465 (1996).

    Article  CAS  Google Scholar 

  2. C. Hölscher, Trends Neurosci. 20, 298 (1997).

    Article  Google Scholar 

  3. O. Palluy and M. Rigaud, Neurosci. Lett. 208, 1 (1996).

    Article  CAS  Google Scholar 

  4. E. Bonfoco, M. Leist, B. Zhivotovsky, S. Orrenius, S.A. Lipton and P. Nicotera, J. Neurochem. 67, 2484 (1996).

    CAS  Google Scholar 

  5. E.M. Grasbon-Frodl and P. Brundin, Exp. Brain Res. 113, 138 (1997).

    Article  CAS  Google Scholar 

  6. P.P. Michel, N. Lambeng and M. Ruberg, Clin. Neuropharmacol. 22, 137 (1999).

    CAS  Google Scholar 

  7. M.P. Murphy, Biochim. Biophys. Acta 1411, 401 (1999).

    Article  CAS  Google Scholar 

  8. S.A. Lipton, Y.B. Choi, Z.H. Pan, S.Z. Lei, H.V. Chen, N.J. Sucher, J. Loscalzo, D.J. Singel and J.S. Stamler, Nature 364, 626 (1993).

    Article  CAS  Google Scholar 

  9. G.C. Brown, Biochim. Biophys. Acta 1411, 351 (1999).

    Article  CAS  Google Scholar 

  10. Y.H. Li, P. Maher and D. Schubert, Neuron 19, 453 (1997).

    Article  CAS  Google Scholar 

  11. M.B. Hansen, S.E. Nielsen and K. Berg, J. Immunol. Methods 119, 203 (1989).

    Article  CAS  Google Scholar 

  12. H.D. Teixeira, R.I. Schumacher and R. Meneghini, Proc. Natl. Acad. Sci. USA 95, 7872 (1998).

    Article  CAS  Google Scholar 

  13. R.J. Mark, J.N. Keller, I. Kruman and M.P. Mattson, Brain Res. 756, 205 (1997).

    Article  CAS  Google Scholar 

  14. Y. Higuchi and S. Matsukawa, Free Radic. Biol. Med. 24, 418 (1998).

    Article  CAS  Google Scholar 

  15. P. Bernardi, L. Scorrano, R. Colonna, V. Petronilli and F. Di Lisa, Eur. J. Biochem. 264, 687 (1999).

    Article  CAS  Google Scholar 

  16. T.T. Wei, C. Chen, J.W. Hou, B.L. Zhao, W.J. Xin and A. Mori, Toxicology 134, 117 (1999).

    Article  CAS  Google Scholar 

  17. J.P. Bolaños, A. Almeida, V. Stewart, S. Peuchen, J.M. Land, J.B. Clark and S.J.R. Heals, J. Neurochem. 68, 2227 (1997).

    Article  Google Scholar 

  18. J.P. Bolaños, S.J.R. Heales, S. Peuchen, J.E. Barker, J.M. Land and J.B. Clark, Free Radic. Biol. Med. 21, 995 (1996).

    Article  Google Scholar 

  19. J.J. Poderoso, M.C. Carreras, C. Lisdero, N. Riobo, F. Schopfer and A. Boveris, Arch. Biochem. Biophys. 328, 85 (1996).

    Article  CAS  Google Scholar 

  20. L.L. Dugan, S.L. Sensi, L.M.T. Canzoniero, S.D. Handran, S.M. Rothman, T.S. Lin, M.P. Goldberg and D.W. Choi, J. Neurosci. 15, 6377 (1995).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Molecular and Cellular Biophysics, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China

    Taotao Wei, Jingwu Hou, Chang Chen & Wenjuan Xin

  2. Molecular and Nano Science Laboratory of Education Ministry, Department of Physics, Tsinghua University, Beijing, 100084, China

    Chunyang Zhang, Hui Ma & Dieyan Chen

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  1. Taotao Wei
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  2. Chunyang Zhang
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  3. Jingwu Hou
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  4. Chang Chen
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  5. Hui Ma
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  6. Dieyan Chen
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  7. Wenjuan Xin
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Wei, T., Zhang, C., Hou, J. et al. Reactive oxygen species are involved in nitric oxide-induced apoptosis in rat cortical neurons. Res Chem Intermed 26, 875–883 (2000). https://doi.org/10.1163/156856700X00363

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  • DOI: https://doi.org/10.1163/156856700X00363

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