The European Physical Journal Special Topics

, Volume 226, Issue 13, pp 2887–2899

Reactive radical-driven bacterial inactivation by hydrogen-peroxide-enhanced plasma-activated-water

  • Songjie Wu
  • Qian Zhang
  • Ruonan Ma
  • Shuang Yu
  • Kaile Wang
  • Jue Zhang
  • Jing Fang
Regular Article
  • 15 Downloads
Part of the following topical collections:
  1. Technological Applications of Microplasmas

Abstract

The combined effects of plasma activated water (PAW) and hydrogen peroxide (H2O2), PAW/HP, in sterilization were investigated in this study. To assess the synergistic effects of PAW/HP, S. aureus was selected as the test microorganism to determine the inactivation efficacy. Also, the DNA/RNA and proteins released by the bacterial suspensions under different conditions were examined to confirm membrane integrity. Additionally, the intracellular pH (pHi) of S. aureus was measured in our study. Electron spin resonance spectroscopy (ESR) was employed to identify the presence of radicals. Finally, the oxidation reduction potential (ORP), conductivity and pH were measured. Our results revealed that the inactivation efficacy of PAW/HP is much greater than that of PAW, while increased H2O2 concentration result in higher inactivation potential. More importantly, as compared with PAW, the much stronger intensity ESR signals and higher ORP in PAW/HP suggests that the inactivation mechanism of the synergistic effects of PAW/HP: more reactive oxygen species (ROS) and reactive nitrogen species (RNS), especially OH and NO radicals, are generated in PAW combined with H2O2 resulting in more deaths of the bacteria.

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Copyright information

© EDP Sciences and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Songjie Wu
    • 1
  • Qian Zhang
    • 2
  • Ruonan Ma
    • 2
  • Shuang Yu
    • 2
  • Kaile Wang
    • 2
  • Jue Zhang
    • 1
    • 2
  • Jing Fang
    • 1
    • 2
  1. 1.College of Engineering, Peking UniversityBeijingP.R. China
  2. 2.Academy for Advanced Interdisciplinary Studies, Peking UniversityBeijingP.R. China

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