The European Physical Journal Special Topics

, Volume 226, Issue 13, pp 2873–2885

Microplasma jet treatment of bovine serum albumin coatings for controlling enzyme and cell attachment

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Part of the following topical collections:
  1. Technological Applications of Microplasmas

Abstract

We investigated a new approach to control protein and cell attachment inside 96-well polystyrene plates. The wells were first coated with bovine serum albumin (BSA) to inhibit cell and protein attachment. The BSA-coated wells were then treated with a helium microplasma jet for increasing times that resulted in gradual removal of BSA from the surface. It was found that the amount of enzyme and cell attachment could be controlled in the wells where BSA was only partially removed by the microplasma jet. In addition to the surface coverage of BSA, the new surface chemistry induced by the microplasma jet treatment also had an important role in the control of enzyme and cell attachment. In summary, microplasma jet treatment of BSA-coated polystyrene wells is a simple and effective method for controlling enzyme and cell attachment. This might find use for high-throughput screening of new cell culture platforms where control over the level protein, enzyme or cell adherence is needed in order to maintain a specific cell function.

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References

  1. 1.
    F. Iza, G.J. Kim, S.M. Lee, J.K. Lee, J.L. Walsh, Y.T. Zhang, M.G. Kong, Plasma Process. Polym. 5, 322 (2008)CrossRefGoogle Scholar
  2. 2.
    S.A. Al-Bataineh, E.J. Szili, P.J. Gruner, C. Priest, H.J. Griesser, N.H. Voelcker, R.D. Short, D.A. Steele, Plasma Process. Polym. 9, 638 (2012)CrossRefGoogle Scholar
  3. 3.
    P.M. Bryant, E.J. Szili, T. Whittle, S.-J. Park, J.G. Eden, S. Al-Bataineh, D.A. Steele, R.D. Short, J.W. Bradley, Surf. Coat. Technol. 204, 2279 (2010)CrossRefGoogle Scholar
  4. 4.
    K.H. Becker, K.H. Schoenbach, J.G. Eden, J. Phys. D: Appl. Phys. 39, R55 (2006)ADSCrossRefGoogle Scholar
  5. 5.
    K.F. Chen, N.P. Ostrom, S.J. Park, J.G. Eden, Appl. Phys. Lett. 88, 061121 (2006)ADSCrossRefGoogle Scholar
  6. 6.
    J.W. Frame, P.C. John, T.A. DeTemple, J.G. Eden, Appl. Phys. Lett. 72, 2634 (1998)ADSCrossRefGoogle Scholar
  7. 7.
    J.G. Eden, S.-J. Park, N.P. Ostrom, K.-F. Chen, J. Phys. D: Appl. Phys. 38, 1644 (2005)ADSCrossRefGoogle Scholar
  8. 8.
    N.P. Ostrom, J.G. Eden, IEEE Trans. Plasma Sci. 33, 576 (2005)ADSCrossRefGoogle Scholar
  9. 9.
    S.J. Park, K.F. Chen, N.P. Ostrom, J.G. Eden, Appl. Phys. Lett. 86, 111501 (2005)ADSCrossRefGoogle Scholar
  10. 10.
    B.J. Ricconi, S.-J. Park, S.H. Sung, P.A. Tchertchian, J.G. Eden, Electron. Lett. 43, 20071673 (2007)CrossRefGoogle Scholar
  11. 11.
    E.J. Szili, S.A. Al-Bataineh, P.M. Bryant, R.D. Short, J.W. Bradley, D.A. Steele, Plasma Process. Polym. 8, 38 (2011)CrossRefGoogle Scholar
  12. 12.
    S. Yonson, S. Coulombe, V. Léveillé, R.L. Leask, J. Phys. D: Appl. Phys. 39, 3508 (2006)ADSCrossRefGoogle Scholar
  13. 13.
    H.M.L. Tan, H. Fukuda, T. Akagi, T. Ichiki, Thin Solid Films 515, 5172 (2007)ADSCrossRefGoogle Scholar
  14. 14.
    J. West, A. Michels, S. Kittel, P. Jacob, J. Franzke, Lab Chip 7, 981 (2007)CrossRefGoogle Scholar
  15. 15.
    Y. Sakiyama, N. Knake, D. Schröder, J. Winter, V.S. der Gathen, D.B. Graves, Appl. Phys. Lett. 97, 151501 (2010)ADSCrossRefGoogle Scholar
  16. 16.
    M. Laroussi, X. Lu, Appl. Phys. Lett. 87, 113902 (2005)ADSCrossRefGoogle Scholar
  17. 17.
    J.K. Evju, P.B. Howell, L.E. Locascio, M.J. Tarlov, J.J. Hickman, Appl. Phys. Lett. 84, 1668 (2004)ADSCrossRefGoogle Scholar
  18. 18.
    C.P. Klages, C. Berger, M. Eichler, M. Thomas, Contrib. Plasma Phys. 47, 49 (2007)ADSCrossRefGoogle Scholar
  19. 19.
    C.-P. Klages, A. Hinze, K. Lachmann, C. Berger, J. Borris, M. Eichler, M. von Hausen, A. Zänker, M. Thomas, Plasma Process. Polym. 4, 208 (2007)CrossRefGoogle Scholar
  20. 20.
    E.J. Szili, S.A. Al-Bataineh, C. Priest, P.J. Gruner, P. Ruschitzka, J.W. Bradley, J. Ralston, D.A. Steele, R.D. Short, Proc. SPIE 8204, 82042J (2011)ADSCrossRefGoogle Scholar
  21. 21.
    S. Thorslund, F. Nikolajeff, J. Micromech. Microeng. 17, N16 (2007)ADSCrossRefGoogle Scholar
  22. 22.
    E.J. Szili, S.A. Al-Bataineh, P. Ruschitzka, G. Desmet, C. Priest, H.J. Griesser, N.H. Voelcker, F.J. Harding, D.A. Steele, R.D. Short, RSC Adv. 2, 12007 (2012)CrossRefGoogle Scholar
  23. 23.
    C. Priest, P.J. Gruner, E.J. Szili, S.A. Al-Bataineh, J.W. Bradley, J. Ralston, D.A. Steele, R.D. Short, Lab Chip 11, 541 (2011)CrossRefGoogle Scholar
  24. 24.
    M. Miclea, K. Kunze, U. Heitmann, S. Florek, J. Franzke, K. Niemax, J. Phys. D: Appl. Phys. 38, 1709 (2005)ADSCrossRefGoogle Scholar
  25. 25.
    J.W. Frame, D.J. Wheeler, T.A. DeTemple, J.G. Eden, Appl. Phys. Lett. 71, 1165 (1997)ADSCrossRefGoogle Scholar
  26. 26.
    S.-J. Park, J. Chen, C. Liu, J.G. Eden, Appl. Phys. Lett. 78, 419 (2001)ADSCrossRefGoogle Scholar
  27. 27.
    K. Becker, A.S.-M. Koutsospyros, S.-M. Yin, C. Christodoulatos, N. Abramzon, J.C. Joaquin, G. Brelles-Mariño, Plasma Phys. Control. Fus. 47, B513 (2005)CrossRefGoogle Scholar
  28. 28.
    M. Miclea, K. Kunze, G. Musa, J. Franzke, K. Niemax, Spectrochim. Acta B 56, 37 (2001)ADSCrossRefGoogle Scholar
  29. 29.
    O. Sakai, Y. Kishimoto, K. Tachibana, J. Phys. D: Appl. Phys. 38, 431 (2005)ADSCrossRefGoogle Scholar
  30. 30.
    M. Teschke, J. Kedzierski, E.G. Finantu-Dinu, D. Korzec, J. Engemann, IEEE Trans. Plasma Sci. 33, 310 (2005)ADSCrossRefGoogle Scholar
  31. 31.
    J.C.T. Eijkel, H. Stoeri, A. Manz, Anal. Chem. 72, 2547 (2000)CrossRefGoogle Scholar
  32. 32.
    M. Laroussi, A. Alexeff, J.P. Richardson, F.F. Dyer, IEEE Trans. Plasma Sci. 30, 158 (2002)ADSCrossRefGoogle Scholar
  33. 33.
    J.J. Shi, M.G. Kong, Appl. Phys. Lett. 90, 111502 (2007)ADSCrossRefGoogle Scholar
  34. 34.
    A.M. Bilgic, U. Engel, E. Voges, M. Kuckelheim, J.A.C. Broekaer, Plasma Sources Sci. Technol. 9, 1 (2000)ADSCrossRefGoogle Scholar
  35. 35.
    P. Siebert, G. Petzold, Á. Hellenbart, J. Müller, Appl. Phys. A 67, 155 (1998)ADSCrossRefGoogle Scholar
  36. 36.
    P. von Allmen, D.J. Sadler, C. Jensen, N.P. Ostrom, S.T. McCain, B.A. Vojak, J.G. Eden, Appl. Phys. Lett. 82, 4447 (2003)ADSCrossRefGoogle Scholar
  37. 37.
    A.D. Koutsospyros, S. Yin, C. Christodoulatos, K. Becker, IEEE Trans. Plasma Sci. 33 42 (2005)ADSCrossRefGoogle Scholar
  38. 38.
    X. Lu, Z. Jiang, Q. Xiong, Z. Tang, X. Hu, Y. Pan, Appl. Phys. Lett. 92, 081502 (2008)ADSCrossRefGoogle Scholar
  39. 39.
    A. Hinze, C.-P. Klages, A. Zänker, M. Thomas, T. Wirth, W.E.S. Unger, Plasma Process. Polym. 5, 460 (2008)CrossRefGoogle Scholar
  40. 40.
    E. Stoffels, I.E. Kieft, R.E.J. Sladek, L.J.M. v d Bedem, E.P. v d Laan, M. Steinbuch, Plasma Sources Sci. Technol. 15, S169 (2006)CrossRefGoogle Scholar
  41. 41.
    Q. Peng, A. Juzeniene, J. Chen, L.O. Svaasand, T. Warloe, K.-E. Giercksky, J. Moan Rep. Progress Phys. 71, 056701 (2008)ADSCrossRefGoogle Scholar
  42. 42.
    H.W. Lee, G.J. Kim, J.M. Kim, J.K. Park, J.K. Lee, G.C. Kim, J. Endodont. 35, 587 (2009)CrossRefGoogle Scholar
  43. 43.
    W.H. Chiang, R.M. Sankaran, Appl. Phys. Lett. 91, 121503 (2007)ADSCrossRefGoogle Scholar
  44. 44.
    P. Wettstein, C. Priest, S.A. Al-Bataineh, R.D. Short, P.M. Bryant, J.W. Bradley, S.P. Low, L. Parkinson, E.J. Szili, Biomicrofluidics 9, 014124 (2015)CrossRefGoogle Scholar
  45. 45.
    S.A. Al-Bataineh, E.J. Szili, A. Mishra, S.-J. Park, J.G. Eden, H.J. Griesser, N.H. Voelcker, R.D. Short, D.A. Steele, Plasma Process. Polym. 8, 695 (2011)CrossRefGoogle Scholar
  46. 46.
    E.J. Szili, S.-H. Hong, R.D. Short, Biointerphases 10, 029511 (2015)CrossRefGoogle Scholar
  47. 47.
    F. Bernsmann, N. Lawrence, M. Hannig, C. Ziegler, H. Gnaser, Anal. Bioanal. Chem. 391, 545 (2008)CrossRefGoogle Scholar
  48. 48.
    J.B. Lhoest, M.S. Wagner, C.D. Tidwell, D.G. Castner, J. Biomed. Mater. Res. 57, 432 (2001)CrossRefGoogle Scholar
  49. 49.
    D.M. Bryant, K.E. Mostov, Nature Rev. 9, 887 (2008)CrossRefGoogle Scholar
  50. 50.
    C.E. Kang, E.J. Gemeinhart, R.A. Gemeinhart, J. of Biomed. Mater. Res. A 71A, 403 (2004)CrossRefGoogle Scholar
  51. 51.
    S. Morgenthaler, C , Zink, N.D. Spencer, Soft Matter 4, 419 (2008)ADSCrossRefGoogle Scholar
  52. 52.
    J. Genzer, R.R. Bhat. Langmuir 24, 2294 (2008)CrossRefGoogle Scholar
  53. 53.
    M. Zelzer, R. Majani, J.W. Bradley, F.R.A.J. Rose, M.C. Davies, M.R. Alexander, Biomaterials 29, 172 (2008)CrossRefGoogle Scholar
  54. 54.
    S.K.W. Dertinger, X. Jiang, Z. Li, V.N. Murthy, G.M. Whitesides, PNAS 99, 12542 (2002)ADSCrossRefGoogle Scholar
  55. 55.
    S.T. Plummer, Q. Wang, P.W. Bohn, R. Stockton, M.A. Schwartz, Langmuir 19, 7528 (2003)CrossRefGoogle Scholar
  56. 56.
    G. Maheshwari, G. Brown, D.A. Lauffenburger, A. Wells, L.G. Griffith, J. Cell Sci. 113, 1677 (2000)Google Scholar
  57. 57.
    W. Wang, R. Eddy, J. Condeelis, Nat. Rev. Cancer 7, 429 (2007)CrossRefGoogle Scholar
  58. 58.
    T. Jin, X. Xu, D. Hereld, Cytokine 44, 1 (2008)CrossRefGoogle Scholar
  59. 59.
    S.A. Eccles, Curr. Opin. Genet. Dev. 15, 77 (2005)CrossRefGoogle Scholar
  60. 60.
    N. Wells, M.A. Baxter, J.E. Turnbull, P. Murray, D. Edgar, K.L. Parry, D.A. Steele, R.D. Short, Biomaterials 30, 1066 (2009)CrossRefGoogle Scholar
  61. 61.
    A. Michelmore, L. Clements, D.A. Steele, N.H. Voelcker, E.J. Szili, J. Nanomater. 2012, 839053 (2012)CrossRefGoogle Scholar

Copyright information

© EDP Sciences and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Future Institute, University of South AustraliaMawson LakesAustralia

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