Abstract.
Liposome suspensions comprising the synthetic cationic lipid DOTAP, the zwitterionic DPPC and the anti-cancer agent Paclitaxel were deposited on solid substrates and investigated by X-ray and neutron reflectivity measurements at ESRF and ILL. Well ordered multilamellar drug/lipid membranes were obtained, such as indicated by the several orders of Bragg reflections in the diffraction pattern. With an excess of Paclitaxel, additional Bragg peaks from the drug crystalline phase could be observed. Changes of the molecular organization in the drug-lipid system were investigated as a function of relative humidity using a custom-built controlled humidity chamber. For DOTAP multilayers the effects of bilayer swelling were much more pronounced than for DPPC under similar conditions, while the crystalline peaks of the drug were not affected. The pronounced swelling of DOTAP multilayers might be related to the electrostatic repulsion between the charged lipid headgroups.
Similar content being viewed by others
References
L.P. Cavalcanti, O. Konovalov, I.L. Torriani, H. Haas, Nucl. Instrum. Methods B 238, 290–293 (2005)
L.P. Cavalcanti, I.L. Torriani, Biophys. Lett. (2006) DOI: 10.1007/s00249-006-0077-3
M. Schmitt-Sody, Clin. Cancer Res. 9, 2335–2341 (2003)
J. Lasch, Liposomes, edited by V. Torchilin, V. Weissig, 2nd edn. (Oxford, 2003), pp. 3–29
L. Perino-Gallice, G. Fragneto, U. Mennicke, T. Salditt, F. Rieutord, Eur. Phys. J. E 8, 275–282 (2002)
A. Asmussen, H. Riegler, J. Chem. Phys. 104, 8151–8158 (1996)
L.P. Cavalcanti, O. Konovalov, I.L. Torriani, Eur. Biophys. J. 35, 431–438 (2006)
M. Sainsbury, The Chemistry of Aantitumor Agents, edited by D. Wilman (Chapman and Hall, 1990), pp. 410–435
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cavalcanti, L., Haas, H., Bordallo, H. et al. In situ X-ray and neutron diffraction study of lipid membrane swelling. Eur. Phys. J. Spec. Top. 141, 217–221 (2007). https://doi.org/10.1140/epjst/e2007-00043-9
Issue Date:
DOI: https://doi.org/10.1140/epjst/e2007-00043-9