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Status of the crystallography beamlines at PETRA III

  • Anja Burkhardt
  • Tim Pakendorf
  • Bernd Reime
  • Jan Meyer
  • Pontus Fischer
  • Nicolas Stübe
  • Saravanan Panneerselvam
  • Olga Lorbeer
  • Karolina Stachnik
  • Martin Warmer
  • Philip Rödig
  • Dennis Göries
  • Alke MeentsEmail author
Regular Article
Part of the following topical collections:
  1. Focus Point on Status of third-generation synchrotron crystallography beamlines: An overview

Abstract.

Since 2013, three beamlines for macromolecular crystallography are available to users at the third-generation synchrotron PETRA III in Hamburg: P11, P13 and P14, the latter two operated by EMBL. Beamline P11 is operated by DESY and is equipped with a Pilatus 6M detector. Together with the photon flux of \( 2\times 10^{13}\) ph/s provided by the very brilliant X-ray source of PETRA III, a full data set can be typically collected in less than 2min. P11 provides state-of-the-art microfocusing capabilities with beam sizes down to \( 1\times 1\) μ m2, which makes the beamline ideally suited for investigation of microcrystals and serial crystallography experiments. An automatic sample changer allows fast sample exchange in less than 20s, which enables high-throughput crystallography and fast crystal screening. For sample preparation, an S2 biosafety laboratory is available in close proximity to the beamline.

Keywords

Beam Size Grazing Angle Beam Position Monitor Macromolecular Crystallography Automatic Sample Changer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    H. Franz, O. Leupold, R. Röhlsberger, S.V. Roth, O.H. Seeck, J. Spengler, J. Strempfer, M. Tischer, J. Viefhaus, E. Weckert, T. Wroblewski, Synchrotron Radiat. News 19, 25 (2006)CrossRefGoogle Scholar
  2. 2.
    K. Balewski, W. Brefeld, W. Decking, Y. Li, G.K. Sahoo, R. Wanzenberg, Proceedings of EPAC, Lucerne, Switzerland, PP. 2302--2304Google Scholar
  3. 3.
  4. 4.
  5. 5.
  6. 6.
  7. 7.
  8. 8.
  9. 9.
    A. Meents, B. Reime, N. Stuebe, P. Fischer, M. Warmer, D. Goeries, J. Roever, J. Meyer, J. Fischer, A. Burkhardt, I. Vartiainen, P. Karvinen, C. David, SPIE Optical Engineering + Applications, Proceedings of SPIE 8851, San Diego, United States, p. 88510K (2013)Google Scholar
  10. 10.
    I. Vartiainen, M. Warmer, D. Goeries, E. Herker, R. Reimer, C. David, A. Meents, J. Synchrotron Radiat. 21, 790 (2014)CrossRefGoogle Scholar
  11. 11.
    K. Stachnik, I. Mohacsi, I. Vartiainen, N. Stuebe, J. Meyer, M. Warmer, C. David, A. Meents, Appl. Phys. Lett. 107, 011105 (2015)ADSCrossRefGoogle Scholar
  12. 12.
  13. 13.
    W. Kabsch, Acta Crystallogr. D 66, 125 (2010)CrossRefGoogle Scholar
  14. 14.
    A.G.W. Leslie, Acta Crystallogr. D 62, 48 (2006)CrossRefGoogle Scholar
  15. 15.
    G.M. Sheldrick, Acta Crystallogr. A 64, 112 (2008)ADSCrossRefGoogle Scholar
  16. 16.
    P.D. Adams, P.V. Afonine, G. Bunkóczi, V.B. Chen, I.W. Davis, N. Echols, J.J. Headd, L.-W. Hung, G.J. Kapral, R.W. Grosse-Kunstleve, A.J. McCoy, N.W. Moriarty, R. Oeffner, R.J. Read, D.C. Richardson, J.S. Richardson, T.C. Terwilliger, P.H. Zwart, Acta Crystallogr. D 66, 213 (2010)CrossRefGoogle Scholar
  17. 17.
    M.D. Winn, C.C. Ballard, K.D. Cowtan, E.J. Dodson, P. Emsley, P.R. Evans, R.M. Keegan, E.B. Krissinel, A.G.W. Leslie, A. McCoy, S.J. McNicholas, G.N. Murshudov, N.S. Pannu, E.A. Potterton, H.R. Powell, R.J. Read, A. Vagin, K.S. Wilson, Acta. Crystallogr. D 67, 235 (2011)CrossRefGoogle Scholar
  18. 18.
    P. Emsley, B. Lohkamp, W.G. Scott, K. Cowtan, Acta Crystallogr. D 66, 486 (2010)CrossRefGoogle Scholar
  19. 19.
    O.V. Dolomanov, L.J. Bourhis, R.J. Gildea, J.A.K. Howard, H. Puschmann, J. Appl. Crystallogr. 42, 339 (2009)CrossRefGoogle Scholar
  20. 20.
    P. Roedig, I. Vartiainen, R. Duman, S. Panneerselvam, N. Stuebe, O. Lorbeer, M. Warmer, G. Sutton, D.I. Stuart, E. Weckert, C. David, A. Wagner, A. Meents, Sci. Rep. 5, 10451 (2015)ADSCrossRefGoogle Scholar
  21. 21.
    K. Schulte, N. Pawlowski, K. Faelber, C. Fröhlich, J. Howard, O. Daumke, BMC Biol. 14, 14 (2016) DOI:10.1186/s12915-016-0236-7 CrossRefGoogle Scholar
  22. 22.
    J. Hellmich, M. Bommer, A. Burkhardt, M. Ibrahim, J. Kern, A. Meents, F. Müh, H. Dobbek, A. Zouni, Structure 22, 1607 (2014)CrossRefGoogle Scholar
  23. 23.
    F. Stellato, D. Oberthür, M. Liang, R. Bean, C. Gati, O. Yefanov, A. Barty, A. Burkhardt, P. Fischer, L. Gallia, R.A. Kirian, J. Meyer, S. Panneerselvam, C.H. Yoona, F. Chervinskii, E. Speller, T.A. White, C. Betzel, A. Meents, H.N. Chapman, IUCrJ 1, 204 (2014)CrossRefGoogle Scholar
  24. 24.
    A. Burkhardt, M. Warmer, S. Panneerselvam, A. Wagner, A. Zouni, C. Glöckner, R. Reimer, H. Hohenberg, A. Meents, Acta Crystallogr. F 68, 495 (2012)CrossRefGoogle Scholar
  25. 25.
    A. Burkhardt, A. Wagner, M. Warmer, R. Reimer, H. Hohenberg, J. Ren, E.E. Fry, D.I. Stuart, A. Meents, Acta Crystallogr. D 69, 308 (2013)CrossRefGoogle Scholar

Copyright information

© Società Italiana di Fisica and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Anja Burkhardt
    • 1
  • Tim Pakendorf
    • 1
  • Bernd Reime
    • 1
  • Jan Meyer
    • 1
  • Pontus Fischer
    • 1
  • Nicolas Stübe
    • 1
  • Saravanan Panneerselvam
    • 1
  • Olga Lorbeer
    • 1
  • Karolina Stachnik
    • 1
  • Martin Warmer
    • 1
  • Philip Rödig
    • 1
  • Dennis Göries
    • 1
  • Alke Meents
    • 1
    Email author
  1. 1.Deutsches Elektronen-Synchrotron DESYPhoton ScienceHamburgGermany

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