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Size-dependent interaction of a 3-arm star poly(ethylene glycol) with two biological nanopores

The European Physical Journal E volume 41, Article number: 77 (2018) Cite this article

Abstract.

We use two pore-forming proteins, alpha-hemolysin and aerolysin, to compare the polymer size-dependence of ionic current block by two types of ethyleneglycol polymers: 1) linear and 2) 3-arm star poly(ethylene glycol), both applied as a polydisperse mixture of average mass 1kDa under high salt conditions. The results demonstrate that monomer size sensitivity, as known for linear PEGs, is conserved for the star polymers with only subtle differences in the dependence of the residual conductance on monomer number. To explain this absence of a dominant effect of polymer architecture, we propose that PEG adsorbs to the inner pore wall in a collapsed, salted-out state, likely due to the effect of hydrophobic residues in the pore wall on the availability of water for hydration.

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Authors and Affiliations

  1. Laboratory for Membrane Physiology and Technology, Department of Physiology, Faculty of Medicine, University of Freiburg, Hermann-Herder-Str. 7, 79104, Freiburg, Germany

    Monasadat Talarimoghari & Jan C. Behrends

  2. Ionera Technologies GmbH, Hermann-Herder-Str. 7, 79104, Freiburg, Germany

    Gerhard Baaken

  3. Institute for Macromolecular Chemistry, Stefan-Meier-Str. 31, 79104, Freiburg, Germany

    Ralf Hanselmann

  4. Freiburg Materials Research Centre, University of Freiburg, Stefan-Meier-Str. 19, 79104, Freiburg, Germany

    Ralf Hanselmann & Jan C. Behrends

  5. Freiburg Centre for Interactive Materials and Bioinspired Technologies, University of Freiburg, Georges-Köhler-Allee 105, 79110, Freiburg, Germany

    Jan C. Behrends

Authors
  1. Monasadat Talarimoghari
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  2. Gerhard Baaken
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  3. Ralf Hanselmann
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  4. Jan C. Behrends
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Corresponding author

Correspondence to Jan C. Behrends.

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This paper is dedicated to the memory of Loïc Auvray: creative scientist, inspiring scholar and genial host of unforgettable gatherings.

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Talarimoghari, M., Baaken, G., Hanselmann, R. et al. Size-dependent interaction of a 3-arm star poly(ethylene glycol) with two biological nanopores. Eur. Phys. J. E 41, 77 (2018). https://doi.org/10.1140/epje/i2018-11687-6

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  • DOI: https://doi.org/10.1140/epje/i2018-11687-6

Keywords

  • Polymers: From Adsorption to Translocation - Topical Issue in Memoriam Loïc Auvray (1956-2016)