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Dynamics of a polyelectrolyte through aerolysin channel as a function of applied voltage and concentration⋆

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Abstract.

We describe the behaviour of a polyelectrolyte in confined geometry. The transport of a polyelectrolyte, dextran sulfate, through a recombinant protein channel, aerolysin, inserted into a planar lipid bilayer is studied as a function of applied voltage and polyelectrolyte concentration and chain length. The aerolysin pore has a weak geometry asymmetry, a high number of charged residues and the polyelectrolyte is strongly negatively charged. The resulting current blockades were characterized by short and long dwelling times. Their frequency varies exponentially as a function of applied voltage and linearly as a function of polyelectrolyte concentration. The long blockade duration decreases exponentially when the electrical force increases. The ratio of the population of short events to the one of long events decreases when the applied voltage increases and displays an exponential variation. The long residence time increases with the polyelectrolyte chain length. We measure a reduction of the effective charge of the polyelectrolyte at the pore entry and inside the channel. For a fixed applied voltage, + / - 100 mV, at both sides of the protein pore entrance, the events frequency is similar as a function of dextran sulfate concentration. The mean blockade durations are independent of polyelectrolyte concentration and are similar for both entrances of the pore and remain constant as a function of the electrical force.

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References

  1. G. Oukhaled, J. Mathé, A.-L. Biance, L. Bacri, J.-M. Betton, D. Lairez, J. Pelta, L. Auvray, Phys. Rev. Lett. 98, 158101 (2007)

    Article  ADS  Google Scholar 

  2. C. Merstorf, B. Cressiot, M. Pastoriza-Gallego, A. Oukhaled, J.-M. Betton, L. Auvray, J. Pelta, ACS Chem. Biol. 7, 652 (2012)

    Article  Google Scholar 

  3. L. Payet, M. Martinho, M. Pastoriza-Gallego, J.-M. Betton, L. Auvray, J. Pelta, J. Mathé, Anal. Chem. 84, 4071 (2012)

    Article  Google Scholar 

  4. M. Pastoriza-Gallego, L. Rabah, G. Gibrat, B. Thiebot, F.G. van der Goot, L. Auvray, J.-M. Betton, J. Pelta, J. Am. Chem. Soc. 133, 2923 (2011)

    Article  Google Scholar 

  5. B. Cressiot, A. Oukhaled, G. Patriarche, M. Pastoriza-Gallego, J.-M. Betton, L. Auvray, M. Muthukumar, L. Bacri, J. Pelta, ACS Nano 6, 6236 (2012)

    Article  Google Scholar 

  6. A. Oukhaled, L. Bacri, M. Pastoriza-Gallego, J.-M. Betton, J. Pelta, ACS Chem. Biol. 7, 1935 (2012)

    Article  Google Scholar 

  7. M. Pastoriza-Gallego, M.-F. Breton, F. Discala, L. Auvray, J.-M. Betton, J. Pelta, ACS Nano 8, 11350 (2014)

    Article  Google Scholar 

  8. L. Movileanu, Trends Biotechnol. 27, 333 (2009)

    Article  Google Scholar 

  9. S. Majd, E.C. Yusko, Y.N. Billeh, M.X. Macrae, J. Yang, M. Mayer, Curr. Opin. Biotechnol. 21, 439 (2010)

    Article  Google Scholar 

  10. H. Wang, J. Ettedgui, J. Forstater, J.W.F. Robertson, J.E. Reiner, H. Zhang, S. Chen, J.J. Kasianowicz, ACS Sens. 3, 251 (2018)

    Article  Google Scholar 

  11. D. Deamer, M. Akeson, D. Branton, Nat. Biotechnol. 34, 518 (2016)

    Article  Google Scholar 

  12. Y. Wang, D. Zheng, Q. Tan, M.X. Wang, L.-Q. Gu, Nat. Nanotechnol. 6, 668 (2011)

    Article  ADS  Google Scholar 

  13. G. Huang, K. Willems, M. Soskine, C. Wloka, G. Maglia, Nat. Commun. 8, 935 (2017)

    Article  ADS  Google Scholar 

  14. J. Nivala, D.B. Marks, M. Akeson, Nat. Biotechnol. 31, 247 (2013)

    Article  Google Scholar 

  15. J.W.F. Robertson, C.G. Rodrigues, V.M. Stanford, K.A. Rubinson, O.V. Krasilnikov, J.J. Kasianowicz, Proc. Natl. Acad. Sci. U.S.A. 104, 8207 (2007)

    Article  ADS  Google Scholar 

  16. C. Cao, Y.-L. Ying, Z.-L. Hu, D.-F. Liao, H. Tian, Y.-T. Long, Nat. Nanotechnol. 11, 713 (2016)

    Article  ADS  Google Scholar 

  17. F. Piguet, H. Oudali, M. Pastoriza-Gallego, P. Manivet, J. Pelta, A. Oukhaled, Nat. Commun. 9, 966 (2018)

    Article  ADS  Google Scholar 

  18. P. Hänggi, P. Talkner, M. Borkovec, Rev. Mod. Phys. 62, 251 (1990)

    Article  ADS  Google Scholar 

  19. W. Sung, P.J. Park, Phys. Rev. Lett. 77, 783 (1996)

    Article  ADS  Google Scholar 

  20. Y. Kantor, M. Kardar, Phys. Rev. E 69, 21806 (2004)

    Article  ADS  Google Scholar 

  21. E. Slonkina, A.B. Kolomeisky, J. Chem. Phys. 118, 7112 (2003)

    Article  ADS  Google Scholar 

  22. M. Muthukumar, Polymer Translocation (CRC Press, 2011)

  23. O. Flomenbom, J. Klafter, Phys. Rev. E 68, 41910 (2003)

    Article  ADS  Google Scholar 

  24. A. Cacciuto, E. Luijten, Phys. Rev. Lett. 96, 238104 (2006)

    Article  ADS  Google Scholar 

  25. S. Matysiak, A. Montesi, M. Pasquali, A.B. Kolomeisky, C. Clementi, Phys. Rev. Lett. 96, 118103 (2006)

    Article  ADS  Google Scholar 

  26. H.H. Katkar, M. Muthukumar, J. Chem. Phys. 148, 24903 (2018)

    Article  Google Scholar 

  27. J. Zimmerberg, V.A. Parsegian, Nature 323, 36 (1986)

    Article  ADS  Google Scholar 

  28. M. Montal, P. Mueller, Proc. Natl. Acad. Sci. U.S.A. 69, 478 (1972)

    Article  Google Scholar 

  29. J.J. Kasianowicz, E. Brandin, D. Branton, D.W. Deamer, Proc. Natl. Acad. Sci. U.S.A. 93, 13770 (1996)

    Article  ADS  Google Scholar 

  30. L. Brun, M. Pastoriza-Gallego, G. Oukhaled, J. Mathé, L. Bacri, L. Auvray, J. Pelta, Phys. Rev. Lett. 100, 158302 (2008)

    Article  ADS  Google Scholar 

  31. G. Gibrat, M. Pastoriza-Gallego, B. Thiebot, M.-F. Breton, L. Auvray, J. Pelta, J. Phys. Chem. B 112, 14687 (2008)

    Article  Google Scholar 

  32. S.E. Henrickson, M. Misakian, B. Robertson, J.J. Kasianowicz, Phys. Rev. Lett. 85, 3057 (2000)

    Article  ADS  Google Scholar 

  33. B. Cressiot, E. Braselmann, A. Oukhaled, A.H. Elcock, J. Pelta, P.L. Clark, ACS Nano 9, 9050 (2015)

    Article  Google Scholar 

  34. L. Payet, M. Martinho, C. Merstorf, M. Pastoriza-Gallego, J. Pelta, V. Viasnoff, L. Auvray, M. Muthukumar, J. Mathé, Biophys. J. 109, 1600 (2015)

    Article  ADS  Google Scholar 

  35. R.J. Murphy, M. Muthukumar, J. Chem. Phys. 126, 51101 (2007)

    Article  ADS  Google Scholar 

  36. M. Fivaz, L. Abrami, Y. Tsitrin, F.G. van der Goot, Curr. Top. Microbiol. Immunol. 257, 35 (2001)

    Google Scholar 

  37. L. Abrami, M. Fivaz, E. Decroly, N.G. Seidah, F. Jean, G. Thomas, S.H. Leppla, J.T. Buckley, F.G. van der Goot, J. Biol. Chem. 273, 32656 (1998)

    Article  Google Scholar 

  38. M.W. Parker, J.T. Buckley, J.P.M. Postma, A.D. Tucker, K. Leonard, F. Pattus, D. Tsernoglou, Nature 367, 292 (1994)

    Article  ADS  Google Scholar 

  39. M.T. Degiacomi, I. Iacovache, L. Pernot, M. Chami, M. Kudryashev, H. Stahlberg, F.G. van der Goot, M. Dal Peraro, Nat. Chem. Biol. 9, 623 (2013)

    Article  Google Scholar 

  40. H.U. Wilmsen, F. Pattus, J.T. Buckley, J. Membr. Biol. 115, 71 (1990)

    Article  Google Scholar 

  41. M. Boukhet, F. Piguet, H. Ouldali, M. Pastoriza-Gallego, J. Pelta, A. Oukhaled, Nanoscale 8, 18352 (2016)

    Article  Google Scholar 

  42. C. Lesieur, S. Frutiger, G. Hughes, R. Kellner, F. Pattus, F.G. van der Goot, J. Biol. Chem. 274, 36722 (1999)

    Article  Google Scholar 

  43. R. Stefureac, Y.-T. Long, H.-B. Kraatz, P. Howard, J.S. Lee, Biochemistry 45, 9172 (2006)

    Article  Google Scholar 

  44. R.I. Stefureac, J.S. Lee, Small 4, 1646 (2008)

    Article  Google Scholar 

  45. C. Cao, J. Yu, Y.-Q. Wang, Y.-L. Ying, Y.-T. Long, Anal. Chem. 88, 5046 (2016)

    Article  Google Scholar 

  46. Z.-L. Hu, Z.-Y. Li, Y.-L. Ying, J. Zhang, C. Cao, Y.-T. Long, H. Tian, Anal. Chem. 90, 4268 (2018)

    Article  Google Scholar 

  47. G. Baaken, I. Halimeh, L. Bacri, J. Pelta, A. Oukhaled, J.C. Behrends, ACS Nano 9, 6443 (2015)

    Article  Google Scholar 

  48. C. Cao, J. Yu, M.-Y. Li, Y.-Q. Wang, H. Tian, Y.-T. Long, Small 13, 1702011 (2017)

    Article  Google Scholar 

  49. G. Oukhaled, L. Bacri, J. Mathé, J. Pelta, L. Auvray, EPL 82, 48003 (2008)

    Article  ADS  Google Scholar 

  50. M. Pastoriza-Gallego, G. Gibrat, B. Thiebot, J.-M. Betton, J. Pelta, Biochim. Biophys. Acta - Biomembranes 1788, 1377 (2009)

    Article  Google Scholar 

  51. J. Zhang, B.I. Shklovskii, Phys. Rev. E 75, 21906 (2007)

    Article  ADS  Google Scholar 

  52. J. Mathé, A. Aksimentiev, D.R. Nelson, K. Schulten, A. Meller, Proc. Natl. Acad. Sci. U.S.A. 102, 12377 (2005)

    Article  ADS  Google Scholar 

  53. L. Auvray, Sur quelques propriétés de solutions de macromolécules rigides et de polyélectrolytes dans un milieu poreux modèle, Thesis (1982)

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Authors and Affiliations

  1. LAMBE UMR 8587, Université Cergy-Pontoise, Université Paris-Seine, 95302, Cergy-Pontoise, France

    Manuela Pastoriza-Gallego & Bénédicte Thiébot

  2. LAMBE UMR 8587, Université Evry, CNRS, CEA, Université Paris-Saclay, 91025, Evry, France

    Laurent Bacri & Juan Pelta

  3. Laboratoire Matière et Systèmes Complexes, Université Paris Diderot, F-75205, Paris, France

    Loïc Auvray

Authors
  1. Manuela Pastoriza-Gallego
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  2. Bénédicte Thiébot
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  3. Laurent Bacri
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  4. Loïc Auvray
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  5. Juan Pelta
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Correspondence to Juan Pelta.

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Pastoriza-Gallego, M., Thiébot, B., Bacri, L. et al. Dynamics of a polyelectrolyte through aerolysin channel as a function of applied voltage and concentration⋆. Eur. Phys. J. E 41, 58 (2018). https://doi.org/10.1140/epje/i2018-11661-4

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