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A detailed guideline for the fabrication of single bacterial probes used for atomic force spectroscopy

The European Physical Journal E volume 38, Article number: 140 (2015) Cite this article

Abstract.

The atomic force microscope (AFM) evolved as a standard device in modern microbiological research. However, its capability as a sophisticated force sensor is not used to its full capacity. The AFM turns into a unique tool for quantitative adhesion research in bacteriology by using “bacterial probes”. Thereby, bacterial probes are AFM cantilevers that provide a single bacterium or a cluster of bacteria as the contact-forming object. We present a step-by-step protocol for preparing bacterial probes, performing force spectroscopy experiments and processing force spectroscopy data. Additionally, we provide a general insight into the field of bacterial cell force spectroscopy.

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References

  1. A.E. Khoury, K. Lam, B. Ellis, J.W. Costerton, ASAIO J. 38, M174 (1992)

    Article  Google Scholar 

  2. J.W. Costerton, P.S. Stewart, E.P. Greenberg, Science 284, 1318 (1999)

    ADS  Article  Google Scholar 

  3. K. Hori, S. Matsumoto, Biochem. Engin. J. 48, 424 (2010) ISSN 1369-703X, invited Review Issue 2010

    Article  Google Scholar 

  4. Y. Seo, W. Jhe, Rep. Prog. Phys. 71, 016101 (2008)

    ADS  Article  Google Scholar 

  5. S. Kang, M. Elimelech, Langmuir 25, 9656 (2009)

    Article  Google Scholar 

  6. P. Loskill, H. Haehl, N. Thewes, C.T. Kreis, M. Bischoff, M. Herrmann, K. Jacobs, Langmuir 28, 7242 (2012)

    Article  Google Scholar 

  7. A. Beaussart, S. El-Kirat-Chatel, P. Herman, D. Alsteens, J. Mahillon, P. Hols, Y. Dufrêne, Biophys. J. 104, 1886 (2013)

    ADS  Article  Google Scholar 

  8. M. Lessel, O. Bäumchen, M. Klos, H. Hähl, R. Fetzer, M. Paulus, R. Seemann, K. Jacobs, Surf. Interface Anal. 47, 557 (2015)

    Article  Google Scholar 

  9. M. Benoit, D. Gabriel, G. Gerisch, H.E. Gaub, Nat. Cell Biol. 2, 313 (2000)

    Article  Google Scholar 

  10. M. Benoit, H.E. Gaub, Cells Tissues Organs 172, 174 (2002)

    Article  Google Scholar 

  11. P.H. Puech, K. Poole, D. Knebel, D.J. Muller, Ultramicroscopy 106, 637 (2006)

    Article  Google Scholar 

  12. J. Helenius, C.P. Heisenberg, H.E. Gaub, D.J. Muller, J. Cell Sci. 121, 1785 (2008)

    Article  Google Scholar 

  13. N. Thewes, P. Loskill, P. Jung, H. Peisker, M. Bischoff, M. Herrmann, K. Jacobs, Beilstein J. Nanotechnol. 5, 1501 (2014)

    Article  Google Scholar 

  14. X. Sheng, Y. Peng Ting, S.O. Pehkonen, J. Colloid Interface Sci. 310, 661 (2007)

    Article  Google Scholar 

  15. W.R. Bowen, R.W. Lovitt, C.J. Wright, J. Colloid Interface Sci. 237, 54 (2001)

    Article  Google Scholar 

  16. N.P. Boks, H.J. Busscher, H.C. van der Mei, W. Norde, Langmuir 24, 12990 (2008)

    Article  Google Scholar 

  17. L. Mei, Y. Ren, H.J. Busscher, Y. Chen, H.C. van der Mei, J. Dental Res. 88, 841 (2009)

    Article  Google Scholar 

  18. W. Zhang, A.G. Stack, Y. Chen, Colloids Surf. B: Biointerfaces 82, 316 (2011)

    Article  Google Scholar 

  19. S.K. Lower, C.J. Tadanier, M.F.H. Jr., Geochim. Cosmochim. Acta 64, 3133 (2000)

    ADS  Article  Google Scholar 

  20. S.K. Lower, M.F. Hochella, T.J. Beveridge, Science 292, 1360 (2001)

    ADS  Article  Google Scholar 

  21. A. Beaussart, S. El-Kirat-Chatel, R.M.A. Sullan, D. Alsteens, P. Herman, S. Derclaye, Y.F. Dufrêne, Nat. Protocols 9, 1049 (2014)

    Article  Google Scholar 

  22. A. Razatos, Y.L. Ong, M.M. Sharma, G. Georgiou, Proc. Natl. Acad. Sci. U.S.A. 95, 11059 (1998)

    ADS  Article  Google Scholar 

  23. R.J. Emerson IV, T.S. Bergstrom, Y. Liu, E.R. Soto, C.A. Brown, W.G. McGimpsey, T.A. Camesano, Langmuir 22, 11311 (2006)

    Article  Google Scholar 

  24. T. Cao, H. Tang, X. Liang, A. Wang, G.W. Auner, S.O. Salley, K. Ng, Biotechnol. Bioengin. 94, 167 (2006)

    Article  Google Scholar 

  25. Y.L. Ong, A. Razatos, G. Georgiou, M.M. Sharma, Langmuir 15, 2719 (1999)

    Article  Google Scholar 

  26. W. Qu, H.J. Busscher, J.M. Hooymans, H.C. Van der Mei, J. Colloid Interface Sci. 358, 430 (2011)

    Article  Google Scholar 

  27. A.L. Neal, T.L. Bank, M.F. Hochella, K.M. Rosso, Geochem. Trans. 6, 77 (2005)

    Article  Google Scholar 

  28. H. Lee, J. Rho, P.B. Messersmith, Adv. Mater. 21, 431 (2009)

    Article  Google Scholar 

  29. E. Velzenberger, I. Pezron, G. Legeay, M.D. Nagel, K.E. Kirat, Langmuir 24, 11734 (2008)

    Article  Google Scholar 

  30. R.L. Meyer, X. Zhou, L. Tang, A. Arpanaei, P. Kingshott, F. Besenbacher, Ultramicroscopy 110, 1349 (2010)

    Article  Google Scholar 

  31. P. Kuusela, Nature 276, 718 (1978)

    ADS  Article  Google Scholar 

  32. A.W. Buck, V.G. Fowler Jr., R. Yongsunthon, J. Liu, A.C. DiBartola, Y.A. Que, P. Moreillon, S.K. Lower, Langmuir 26, 10764 (2010)

    Article  Google Scholar 

  33. G. Zeng, T. Mu ller, R.L. Meyer, Langmuir 30, 4019 (2014)

    Article  Google Scholar 

  34. W.R. Bowen, A.S. Fenton, R.W. Lovitt, C.J. Wright, Biotechnol. Bioengin. 79, 170 (2002)

    Article  Google Scholar 

  35. S.B. Velegol, B.E. Logan, Langmuir 18, 5256 (2002)

    Article  Google Scholar 

  36. G.A. Burks, S.B. Velegol, E. Paramonova, B.E. Lindenmuth, J.D. Feick, B.E. Logan, Langmuir 19, 2366 (2003)

    Article  Google Scholar 

  37. H. Lee, S.M. Dellatore, W.M. Miller, P.B. Messersmith, Science 318, 426 (2007)

    ADS  Article  Google Scholar 

  38. P.C. Lau, J.R. Dutcher, T.J. Beveridge, J.S. Lam, Biophys. J 96, 2935 (2009)

    Article  Google Scholar 

  39. P.C. Lau, T. Lindhout, T.J. Beveridge, J.R. Dutcher, J.S. Lam, J. Bacteriol. 191, 6618 (2009)

    Article  Google Scholar 

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Author information

Affiliations

  1. Experimental Physics, Campus E2 9, Saarland University, D-66123, Saarbrücken, Germany

    Nicolas Thewes, Peter Loskill, Christian Spengler, Sebastian Hümbert & Karin Jacobs

  2. Institute of Medical Microbiology and Hygiene, Saarland University, D-66421, Homburg/Saar, Germany

    Markus Bischoff

Authors
  1. Nicolas Thewes
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  2. Peter Loskill
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  3. Christian Spengler
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  4. Sebastian Hümbert
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  5. Markus Bischoff
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  6. Karin Jacobs
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Correspondence to Karin Jacobs.

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Thewes, N., Loskill, P., Spengler, C. et al. A detailed guideline for the fabrication of single bacterial probes used for atomic force spectroscopy. Eur. Phys. J. E 38, 140 (2015). https://doi.org/10.1140/epje/i2015-15140-2

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  • DOI: https://doi.org/10.1140/epje/i2015-15140-2

Keywords

  • Tips and Tricks