Viscosity and drag force involved in organelle transport: Investigation of the fluctuation dissipation theorem

  • K. Hayashi
  • C. G. Pack
  • M. K. Sato
  • K. Mouri
  • K. Kaizu
  • K. Takahashi
  • Y. Okada
Regular Article

Abstract

We observed the motion of an organelle transported by motor proteins in cells using fluorescence microscopy. Particularly, among organelles, the mitochondria in PC12 cells were studied. A mitochondrion was dragged at a constant speed for several seconds without pausing. We investigated the fluctuation dissipation theorem for this constant drag motion by comparing it with the motion of Brownian beads that were incorporated into the cells by an electroporation method. We estimated the viscosity value inside cells from the diffusion coefficients of the beads. Then the viscosity value obtained by using the beads was found to be slightly lower than that obtained from the diffusion coefficient for the organelle motion via the Einstein relation. This discrepancy indicates the violation of the Einstein relation for the organelle motion.

Graphical abstract

Keywords

Living systems: Structure and Function 

Supplementary material

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Supplementary material

Supplementary material

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Supplementary material

10189_2013_9950_MOESM5_ESM.pdf (631 kb)
Supplementary material

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Copyright information

© EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • K. Hayashi
    • 1
  • C. G. Pack
    • 2
  • M. K. Sato
    • 3
  • K. Mouri
    • 2
  • K. Kaizu
    • 4
  • K. Takahashi
    • 4
    • 5
  • Y. Okada
    • 4
  1. 1.School of EngineeringTohoku UniversitySendaiJapan
  2. 2.Cellular Informatics LabRIKENWakoJapan
  3. 3.Institute of Multidisciplinary Research for Advanced MaterialTohoku UniversitySendaiJapan
  4. 4.Quantitative Biology CenterRIKENSuitaJapan
  5. 5.Institute for Advanced BiosciencesKeio UniversityFujisawaJapan

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