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Finite-size corrections to scaling behavior in sorted cell aggregates

The European Physical Journal E volume 33, pages 99–103 (2010)Cite this article

Abstract.

Cell sorting is a widespread phenomenon pivotal to the early development of multicellular organisms. In vitro cell sorting studies have been instrumental in revealing the cellular properties driving this process. However, these studies have as yet been limited to two-dimensional analysis of three-dimensional cell sorting events. Here we describe a method to record the sorting of primary zebrafish ectoderm and mesoderm germ layer progenitor cells in three dimensions over time, and quantitatively analyze their sorting behavior using an order parameter related to heterotypic interface length. We investigate the cell population size dependence of sorted aggregates and find that the germ layer progenitor cells engulfed in the final configuration display a relationship between total interfacial length and system size according to a simple geometrical argument, subject to a finite-size effect.

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Affiliations

  1. Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, D-01307, Dresden, Germany

    A. V. Klopper & S. W. Grill

  2. Max Planck Institute for the Physics of Complex Systems, Nöthnitzer Straße 38, D-01187, Dresden, Germany

    A. V. Klopper & S. W. Grill

  3. Institute of Science and Technology Austria, Am Campus 1, A-3400, Klosterneuburg, Austria

    G. Krens & C. -P. Heisenberg

Authors
  1. A. V. Klopper
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  2. G. Krens
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  3. S. W. Grill
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  4. C. -P. Heisenberg
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Klopper, A.V., Krens, G., Grill, S.W. et al. Finite-size corrections to scaling behavior in sorted cell aggregates. Eur. Phys. J. E 33, 99–103 (2010). https://doi.org/10.1140/epje/i2010-10642-y

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  • DOI: https://doi.org/10.1140/epje/i2010-10642-y

Keywords

  • Sorting
  • PDMS
  • Cell Sorting
  • System Size
  • Mesoderm Cell