Skip to main content
SpringerLink
Log in

γH2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity – preliminary methodological study and discussion

  • Regular Article
  • Published:
The European Physical Journal D Aims and scope Submit manuscript

Abstract

In order to improve patients’ post-treatment quality of life, a shift from surgery to non-surgical (chemo)radio-treatment is recognized in head and neck oncology. However, about half of HNSCC tumors are resistant to irradiation and an efficient marker of individual tumor radiosensitivity is still missing. We analyzed whether various parameters of DNA double strand break (DSB) repair determined in vitro can predict, prior to clinical treatment initiation, the radiosensitivity of tumors. We compared formation and decrease of γH2AX/53BP1 foci in 48 h after irradiating tumor cell primocultures with 2 Gy of γ-rays. To better understand complex tumor behavior, three different cell type primocultures – CD90−, CD90+, and a mixed culture of these cells – were isolated from 1 clinically radioresistant, 2 radiosensitive, and 4 undetermined HPV–HNSCC tumors and followed separately. While DSB repair was delayed and the number of persisting DSBs increased in the radiosensitive tumors, the results for the radioresistant tumor were similar to cultured normal human skin fibroblasts. Hence, DSB repair kinetics/efficiency may correlate with clinical response to radiotherapy for a subset of HNSCC tumors but the size (and therefore practical relevance) of this subset remains to be determined. The same is true for contribution of different cell type primocultures to tumor radioresistance.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. H. Binková, Otorinologie a foniatrie 59, 114 (2010)

    Google Scholar 

  2. Z. Horáková, Otorinologie a foniatrie 59, 107 (2010)

    Google Scholar 

  3. I. Falkova, Zdravotníctvo a Sociálna práca 11, 19 (2016)

    Google Scholar 

  4. F. Perri, Head Neck 37, 763 (2015)

    Article  Google Scholar 

  5. G. Mountzios, Ann. Oncol. 25, 1889 (2014)

    Article  Google Scholar 

  6. W. Han, in Advances in Genetics Research, edited by K.V. Urbano (Nova Science, 2010)

  7. T. Ettl, Oral Oncol. 51, 158 (2015)

    Article  Google Scholar 

  8. G. Peng, Mol. Med. Rep. 10, 1709 (2014)

    Article  Google Scholar 

  9. M. Maalouf, Int. J. Radiat. Oncol. Biol. Phys. 74, 200 (2009)

    Article  Google Scholar 

  10. Q. Wang, Int. J. Cancer 128, 1546 (2011)

    Article  Google Scholar 

  11. T. Kuilman, Genes Dev. 24, 2463 (2010)

    Article  Google Scholar 

  12. A. Lujambio, Bioessays 38, S56 (2016)

    Article  Google Scholar 

  13. L. Ježková, Appl. Radiat. Isot. 83(Pt B), 128 (2014)

    Article  Google Scholar 

  14. M. Falk, Crit. Rev. Eukaryot. Gene Expr. 24, 225 (2014)

    Article  Google Scholar 

  15. J. Sevcik, Cell Signal. 24, 1023 (2012)

    Article  Google Scholar 

  16. J. Sevcik, Cell Signal. 25, 1186 (2013)

    Article  Google Scholar 

  17. A. Affolter, Oncol. Rep. 29, 785 (2013)

    Article  Google Scholar 

  18. X. Ji, Int. J. Clin. Exp. Med. 8, 7002 (2015)

    Google Scholar 

  19. V. Salvatore, Oncotarget 1 (2016)

  20. M. Falk, Appl. Radiat. Isot. 83, 177 (2014)

    Article  Google Scholar 

  21. M. Falk, Biochim. Biophys. Acta 1773, 1534 (2007)

    Article  Google Scholar 

  22. M. Kozubek, Cytometry 45, 1 (2001)

    Article  Google Scholar 

  23. P. Matula, in IEEE International Symposium on Biomedical Imaging: from Nano to Macro 2009 ISBI 09 (2009), p. 1138

  24. M. Svobodova, Oncotarget, 2017, https://doi.org/10.18632/oncotarget.19914

  25. B. Joshua, Head Neck 34, 42 (2012)

    Article  Google Scholar 

  26. M. Falk, Mutat. Res. 704, 88 (2010)

    Article  Google Scholar 

  27. M. Hofer, J. Med. Chem. 59, 3003 (2016)

    Article  Google Scholar 

  28. G. Wolf, Ear Nose Throat J. 80, 897 (2001)

    Google Scholar 

  29. M. Raudenska, Tumour Biol. 36, 9929 (2015)

    Article  Google Scholar 

  30. E. Lukásová, Chromosoma 112, 221 (2004)

    Article  Google Scholar 

  31. E. Lukášová, Biochim. Biophys. Acta 1833, 767 (2013)

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology and Radiobiology, Institute of Biophysics of CAS, 61265, Brno, Czech Republic

    Martin Falk, Olga Kopecna, Daniel Depes, Alena Bacikova & Iva Falkova

  2. Department of Otorhinolaryngology and Head and Neck Surgery, St. Anne’s University Hospital and Faculty of Medicine, Masaryk University, 65691, Brno, Czech Republic

    Zuzana Horakova & Hana Binkova

  3. Department of Pathological Physiology, Faculty of Medicine, Masaryk University, 62500, Brno, Czech Republic

    Marketa Svobodova, Michal Masarik, Jaromir Gumulec & Martina Raudenska

  4. Department of Physiology, Faculty of Medicine, Masaryk University, 62500, Brno, Czech Republic

    Marketa Svobodova, Michal Masarik, Jaromir Gumulec & Martina Raudenska

Authors
  1. Martin Falk
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zuzana Horakova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marketa Svobodova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michal Masarik
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Olga Kopecna
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jaromir Gumulec
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Martina Raudenska
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Daniel Depes
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Alena Bacikova
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Iva Falkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Hana Binkova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Martin Falk.

Additional information

Contribution to the Topical Issue “Dynamics of Systems at the Nanoscale”, edited by Andrey Solov’yov and Andrei Korol.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Falk, M., Horakova, Z., Svobodova, M. et al. γH2AX/53BP1 foci as a potential pre-treatment marker of HNSCC tumors radiosensitivity – preliminary methodological study and discussion. Eur. Phys. J. D 71, 241 (2017). https://doi.org/10.1140/epjd/e2017-80073-2

Download citation

  • Received:

  • Revised:

  • Published:

  • DOI: https://doi.org/10.1140/epjd/e2017-80073-2

Search

Navigation