Abstract
A helium atmospheric pressure plasma jet (APPJ) source with a dielectric capillary and two tubular electrodes was used to induce damage in aqueous plasmid DNA. The fraction of different types of DNA damage (i.e., intact or undamaged, double strand breaks (DSBs), and single strand breaks (SSBs)) that occurred as the result of plasma irradiation was quantified through analysis of agarose gel electrophoresis images. The total DNA damage increased with an increase in both flow rate and duration of irradiation, but decreased with an increase in distance between the APPJ and sample. The average power of the plasma was calculated and the length of APPJ was measured for various flow rates and voltages applied. The possible effects of plasma power and reactive species on DNA damage are discussed.
Graphical abstract
Similar content being viewed by others
References
T. von Woedtke, S. Reuter, K. Masur, K.-D. Weltmann, Phys. Rep. 530, 291 (2013)
K. Arjunan, V. Sharma, S. Ptasinska, Int. J. Mol. Sci. 16, 2971 (2015)
D.B. Graves, Phys. Plasmas 21, 080901 (2014)
E. Stoffels, I.E. Kieft, R.E.J. Sladek, J. Phys. D. 36, 2908 (2003)
K.R. Stalder, J. Woloszko, Contrib. Plasma Phys. 47, 64 (2007).
X. Zhang, M. Li, R. Zhou, K. Feng, S. Yang, Appl. Phys. Lett. 93, 021502 (2008)
M. Laroussi, Plasma Process. Polym. 2, 391 (2005)
T. Sato, O. Furuya, K. Ikeda, T. Nakatani, Plasma Process. Polym. 5, 606 (2008)
T. Sato, T. Miyahara, A. Doi, S. Ochiai, T. Urayama, T. Nakatani, Appl. Phys. Lett. 89, 88 (2006)
E. Dolezalova, P. Lukes, Bioelectrochemistry 103, 7 (2015)
G. Fridman, M. Peddinghaus, M. Balasubramanian, H. Ayan, A. Fridman, A. Gutsol, A. Brooks, Plasma Chem. Plasma Process. 26, 425 (2006)
T. Von Woedtke, H.R. Metelmann, K.D. Weltmann, Contrib. Plasma Phys. 54, 104 (2014)
G. Isbary, G. Morfill, H.U. Schmidt, M. Georgi, K. Ramrath, J. Heinlin, S. Karrer, M. Landthaler, T. Shimizu, B. Steffes, W. Bunk, R. Monetti, J.L. Zimmermann, R. Pompl, W. Stolz, Br. J. Dermatol. 163, 78 (2010)
I.E. Kieft, J.L.V. Broers, V. Caubet-Hilloutou, D.W. Slaaf, F.C.S. Ramaekers, E. Stoffels, Bioelectromagnetics 25, 362 (2004)
M. Vandamme, E. Robert, S. Lerondel, V. Sarron, D. Ries, S. Dozias, J. Sobilo, D. Gosset, C. Kieda, B. Legrain, J.M. Pouvesle, A. Le Pape, Int. J. Cancer 130, 2185 (2012)
G.J. Kim, W. Kim, K.T. Kim, J.K. Lee, Appl. Phys. Lett. 96, 021502 (2010)
X. Han, M. Klas, Y. Liu, M. Sharon Stack, S. Ptasinska, Appl. Phys. Lett. 102, 233703 (2013)
E.A. Ratovitski, X. Cheng, D. Yan, J.H. Sherman, J. Canady, B. Trink, M. Keidar, Plasma Process. Polym. 11, 1128 (2014)
S. Mohades, M. Laroussi, J. Sears, N. Barekzi, H. Razavi, Phys. Plasmas 22, 122001 (2015)
S. Arndt, M. Landthaler, J.L. Zimmermann, P. Unger, E. Wacker, T. Shimizu, Y.F. Li, G.E. Morfill, A.K. Bosserhoff, S. Karrer, PLoS One 10, 1 (2015)
G. Fridman, A. Shereshevsky, M.M. Jost, A.D. Brooks, A. Fridman, A. Gutsol, V. Vasilets, G. Friedman, Plasma Chem. Plasma Process. 27, 163 (2007)
J. Heinlin, G. Isbary, W. Stolz, G. Morfill, M. Landthaler, T. Shimizu, B. Steffes, T. Nosenko, J.L. Zimmermann, S. Karrer, J. Eur. Acad. Dermatol. Venereol. 25, 1 (2011)
G. Fridman, G. Friedman, A. Gutsol, A.B. Shekhter, V.N. Vasilets, A. Fridman, Plasma Process. Polym. 5, 503 (2008)
H.W. Lee, G.Y. Park, Y.S. Seo, Y.H. Im, S.B. Shim, H.J. Lee, J. Phys. D 44, 053001 (2011)
G.Y. Park, S.J. Park, M.Y. Choi, I.G. Koo, J.H. Byun, J.W. Hong, J.Y. Sim, G.J. Collins, J.K. Lee, Plasma Sources Sci. Technol. 21, 043001 (2012)
M.G. Kong, G. Kroesen, G. Morfill, T. Nosenko, T. Shimizu, J. van Dijk, J.L. Zimmermann, New J. Phys. 11, 115012 (2009)
G. Lloyd, G. Friedman, S. Jafri, G. Schultz, A. Fridman, K. Harding, Plasma Process. Polym. 7, 194 (2010)
M. Keidar, Plasma Sources Sci. Technol. 24, 033001 (2015)
X. Han, W.A. Cantrell, E.E. Escobar, S. Ptasinska, Eur. Phys. J. D 68, 46 (2014)
D. O’Connell, L.J. Cox, W.B. Hyland, S.J. McMahon, S. Reuter, W.G. Graham, T. Gans, F.J. Currell, Appl. Phys. Lett. 98, 043701 (2011)
M.Y. Alkawareek, H. Alshraiedeh, S. Higginbotham, P.B. Flynn, Q.T. Algwari, S.P. Gorman, W.G. Graham, B.F. Gilmore, Plasma Med. 4, 211 (2014)
S. Ptasińska, B. Bahnev, A. Stypczyńska, M. Bowden, N.J. Mason, N.S.J. Braithwaite, Phys. Chem. Chem. Phys. 12, 7779 (2010)
X. Yan, F. Zou, X.P. Lu, G. He, M.J. Shi, Q. Xiong, X. Gao, Z. Xiong, Y. Li, F.Y. Ma, M. Yu, C.D. Wang, Y. Wang, G. Yang, Appl. Phys. Lett. 95, 083702 (2009)
A. Stypczynska, S. Ptasinska, B. Bahnev, M. Bowden, N.S.J. Braithwaite, N.J. Mason, Chem. Phys. Lett. 500, 313 (2010)
J.Y. Kim, D. Lee, J. Ballato, W. Cao, S. Kim, Appl. Phys. Lett. 101, 224101 (2012)
B. Bahnev, M.D. Bowden, A. Stypczyńska, S. Ptasińska, N.J. Mason, N.S.J. Braithwaite, Eur. Phys. J. D 68, 140 (2014)
H. Kurita, S. Miyachika, H. Yasuda, K. Takashima, A. Mizuno, Appl. Phys. Lett. 107, 263702 (2015)
H. Kurita, T. Nakajima, H. Yasuda, K. Takashima, A. Mizuno, J.I.B. Wilson, S. Cunningham, Appl. Phys. Lett. 99, 191504 (2011)
K. Niemi, C. O’Neill, L.J. Cox, J. Waskoenig, W.B. Hyland, S.J. McMahon, S. Reuter, F.J. Currell, W.G. Graham, D. O’Connell, T. Gans, AIP Conf. Proc. 1438, 23 (2012)
X. Zhang, S. Ptasinska, J. Phys. D 47, 145202 (2014)
W.-C. Zhu, Q. Li, X.-M. Zhu, Y.-K. Pu, J. Phys. D. 42, 202002 (2009)
A. Schmidt-Bleker, S.A. Norberg, J. Winter, E. Johnsen, S. Reuter, K.D. Weltmann, M.J. Kushner, Plasma Sources Sci. Technol. 24, 035022 (2015)
M. Klas, S. Ptasinska, Plasma Sources Sci. Technol. 22, 025013 (2013)
N. Mericam-Bourdet, M. Laroussi, A. Begum, E. Karakas, J. Phys. D. 42, 055207 (2009)
S. Lehnert, Biomolecular Action of Ionizing Radiation, 1st edn. (Taylor & Francis Group, New York, London, 2008)
Q. Xiong, X.P. Lu, K. Ostrikov, Y. Xian, C. Zou, Z. Xiong, Y. Pan, Phys. Plasmas 17, 043506 (2010)
C. Giustranti, C. Perez, S. Rousset, E. Balanzat, E. Sage, J. Chim. Phys. 96, 132 (1999)
J. Jarrige, M. Laroussi, E. Karakas, Plasma Sources Sci. Technol. 19, 065005 (2010)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Adhikari, E., Ptasinska, S. Correlation between helium atmospheric pressure plasma jet (APPJ) variables and plasma induced DNA damage. Eur. Phys. J. D 70, 180 (2016). https://doi.org/10.1140/epjd/e2016-70274-6
Received:
Revised:
Published:
DOI: https://doi.org/10.1140/epjd/e2016-70274-6