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Polarization-shaped laser pulses for improved fluorescence anisotropy contrast

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Abstract

We present a method to change the fluorescence contrast between two dyes with high fluorescence anisotropy in the same solution under different polarization directions by using polarization-shaped pulses. First a theoretical relationship between contrast, anisotropy and polarization is derived for this case. Then the wavelength dependent polarization anisotropy and the change of contrast by phase-shaping for a solution of Rhodamine B and Stilbene 3 in glycerol is shown. Finally, the application of the polarization-shaped pulses is demonstrated.

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Authors and Affiliations

  1. Institut für Experimentalphysik, Freie Universität Berlin, Arnimallee 14, 14195, Berlin, Germany

    Georg Achazi, Nils Hermes, Alexander Patas, Daniel Tolksdorf & Albrecht Lindinger

Authors
  1. Georg Achazi
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  2. Nils Hermes
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  3. Alexander Patas
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  4. Daniel Tolksdorf
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  5. Albrecht Lindinger
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Correspondence to Georg Achazi.

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Achazi, G., Hermes, N., Patas, A. et al. Polarization-shaped laser pulses for improved fluorescence anisotropy contrast. Eur. Phys. J. D 67, 160 (2013). https://doi.org/10.1140/epjd/e2013-30758-5

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  • DOI: https://doi.org/10.1140/epjd/e2013-30758-5

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