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Soft X-ray contact microscopy of nematode Caenorhabditis elegans

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Abstract.

Soft X-ray Contact Microscopy (SXCM) of Caenorhabditis elegans nematodes with typical length \(\sim 800 \mu\)m and diameter \(\sim 30 \mu\)m has been performed using the PALS laser source of wavelength \(\lambda = 1.314 \mu \)m and pulse duration \(\tau ({\rm FWHM}) = 400\) ps. Pulsed soft X-rays were generated using molybdenum and gold targets with laser intensities \(I \ge 10^{14}\) W/cm2. Images have been recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Cuticle features and several internal organs have been identified in the SXCM images including lateral field, cuticle annuli, pharynx, and hypodermal and neuronal cell nuclei.

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Authors and Affiliations

  1. INFM, CIMAINA, and Istituto di Fisiologia Generale e Chimica Biologica, Universitá di Milano, Via Trentacoste 2, 20134, Milano, Italy

    G. Poletti & F. Orsini

  2. Dipartimento di Fisica “G.Occhialini”, Universitá di Milano Bicocca, Piazza della Scienza 3, 20126, Milano, Italy

    D. Batani, A. Bernardinello & T. Desai

  3. PALS Research Centre, Za Slovankou 3, 18221, Prague 8, Czech Republic

    J. Ullschmied, J. Skala, B. Kralikova, E. Krousky, L. Juha, M. Pfeifer, Ch. Kadlec, T. Mocek, A. Präg & O. Renner

  4. Dipartimento di Biologia, Universitá di Milano, Via Celoria 26, 20133, Milano, Italy

    F. Cotelli & C. Lora Lamia

  5. Dipartimento di Biotecnologie e Bioscienze, Universitá di Milano Bicocca, Piazza della Scienza 2, 20126, Milano, Italy

    A. Zullini

Authors
  1. G. Poletti
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  2. F. Orsini
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  3. D. Batani
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  4. A. Bernardinello
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  5. T. Desai
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  6. J. Ullschmied
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  7. J. Skala
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  8. B. Kralikova
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  9. E. Krousky
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  10. L. Juha
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  11. M. Pfeifer
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  12. Ch. Kadlec
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  13. T. Mocek
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  14. A. Präg
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  15. O. Renner
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  16. F. Cotelli
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  17. C. Lora Lamia
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  18. A. Zullini
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Corresponding author

Correspondence to G. Poletti.

Additional information

Received: 18 February 2004, Published online: 29 June 2004

PACS:

42.62.Be Biological and medical applications - 07.85.Tt X-ray microscopes - 87.59.Bh X-ray radiography

A color version of the figures is available in electronic form at http://www.edpscience.org.

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Poletti, G., Orsini, F., Batani, D. et al. Soft X-ray contact microscopy of nematode Caenorhabditis elegans. Eur. Phys. J. D 30, 235–241 (2004). https://doi.org/10.1140/epjd/e2004-00084-4

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  • DOI: https://doi.org/10.1140/epjd/e2004-00084-4

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