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The European Physical Journal Special Topics

, Volume 227, Issue 10–11, pp 999–1014 | Cite as

Analysis of spontaneous activity in neuronal cultures through recurrence plots: impact of varying connectivity

  • Elisenda Tibau
  • Jordi SorianoEmail author
Regular Article
Part of the following topical collections:
  1. Advances in Nonlinear Dynamics of Complex Networks: Adaptivity, Stochasticity, Delays

Abstract

We analyzed spontaneous activity of cortical neuronal networks in vitro using recurrence plots (RPs). Our data encompasses fluorescence traces of average network activity from two experimental explorations, namely the development of connections during the maturation of the network and the gradual weakening of connections through chemical action. The dynamical richness of the networks in these connectivity-evolving scenarios was examined through recurrence quantification analysis. Measures such as determinism and laminarity were used to portray the degree of uniformity and periodicity of the spontaneous activity patterns. The analysis shows that RPs are a powerful tool to visualize and interpret neuronal networks dynamics, and pinpoint its hallmarks.

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Copyright information

© EDP Sciences, Società Italiana di Fisica and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Departament de Física de la Matèria CondensadaUniversitat de BarcelonaBarcelonaSpain
  2. 2.Universitat de Barcelona Institute of Complex Systems (UBICS)BarcelonaSpain

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