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The European Physical Journal Special Topics

, Volume 223, Issue 10, pp 2023–2033 | Cite as

Using the atomic force microscope as a nanomechanical partner to support evanescent field imaging

  • S. Amini
  • Z. Sun
  • G. A. Meininger
  • K. E. Meissner
Regular Article Resonant Geometries/Structures
Part of the following topical collections:
  1. Taking Detection to the Limit: Biosensing with Optical Microcavities

Abstract

Quantum Dot (QD)/microsphere structures supporting Whispering Gallery Modes (WGMs) are attached to Atomic Force Microscope (AFM) cantilevers for characterization of the evanescent field around the QD/microsphere and utilization of the evanescent field for sensing at the apical surface of live cells. Following laser excitation, QD emission couples to WGMs that circumnavigate the microsphere via total internal reflection at the internal surfaces of the microsphere. The resulting evanescent field is characterized utilizing the high spatial control of an AFM in approaching a dye monolayer on a test surface. The measured evanescent field extends approximately 50 nm from the microsphere surface, matching theoretical predictions. This system was then used to sense the accumulation of integrin and formation of focal adhesions at the apical surface of cells.

Keywords

Atomic Force Microscopy Integrin Vascular Smooth Muscle Cell European Physical Journal Special Topic Whisper Gallery Mode 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© EDP Sciences and Springer 2014

Authors and Affiliations

  • S. Amini
    • 1
  • Z. Sun
    • 2
  • G. A. Meininger
    • 2
  • K. E. Meissner
    • 1
  1. 1.Biomedical Engineering DepartmentTexas A&M UniversityCollege StationUSA
  2. 2.Dalton Cardiovascular Research Center and Department of Medical Pharmacology and PhysiologyUniversity of MissouriColumbiaUSA

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