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The European Physical Journal Special Topics

, Volume 167, Issue 1, pp 191–197 | Cite as

The structural variety of DNA-DPPC-divalent metal cation aggregates: SAXD and SANS study

  • D. Uhríková
  • P. Pullmannová
  • N. Kučerka
  • S. S. Funari
  • J. Teixeira
  • P. Balgavý
Regular Article

Abstract

We examine the structure of aggregates formed due to DNA interaction with dipalmitoylphosphatidylcholine (DPPC) in presence of Ca2+ and Zn2+ using small-angle synchrotron X-ray diffraction (SAXD) and neutron scattering (SANS). SAXD shows structural heterogeneity as a function of the cation concentration and temperature: At low cation concentration (∼1 mM), aggregates show two DPPC phases, one with a lateral segregation of DNA and cation, while higher cation concentration improves the DNA packing and the condensed lamellar phase is observed in DNA+DPPC+20mMion2+ aggregates. The SANS detected the dissolution of the condensed lamellar phase into unilamellar DPPC+Zn2+ vesicles due to gel ↦ liquid-crystal phase transition in DNA+DPPC+20mM Zn2+ aggregates with the short fragmented salmon sperm DNA.

Keywords

European Physical Journal Special Topic DPPC Repeat Distance DPPC Bilayer Lateral Segregation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© EDP Sciences and Springer 2009

Authors and Affiliations

  • D. Uhríková
    • 1
  • P. Pullmannová
    • 1
  • N. Kučerka
    • 1
    • 2
  • S. S. Funari
    • 3
  • J. Teixeira
    • 4
  • P. Balgavý
    • 1
  1. 1.Faculty of Pharmacy, Comenius UniversityBratislavaSlovakia
  2. 2.Canadian Neutron Beam Centre, National Research Council, Chalk RiverOntarioCanada
  3. 3.HASYLAB, DESYHamburgGermany
  4. 4.Laboratoire Léon Brillouin (CEA-CNRS), CEA SaclayGif-sur-Yvette CedexFrance

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