The European Physical Journal Special Topics

, Volume 167, Issue 1, pp 191–197 | Cite as

The structural variety of DNA-DPPC-divalent metal cation aggregates: SAXD and SANS study

  • D. Uhríková
  • P. Pullmannová
  • N. Kučerka
  • S. S. Funari
  • J. Teixeira
  • P. Balgavý
Regular Article


We examine the structure of aggregates formed due to DNA interaction with dipalmitoylphosphatidylcholine (DPPC) in presence of Ca2+ and Zn2+ using small-angle synchrotron X-ray diffraction (SAXD) and neutron scattering (SANS). SAXD shows structural heterogeneity as a function of the cation concentration and temperature: At low cation concentration (∼1 mM), aggregates show two DPPC phases, one with a lateral segregation of DNA and cation, while higher cation concentration improves the DNA packing and the condensed lamellar phase is observed in DNA+DPPC+20mMion2+ aggregates. The SANS detected the dissolution of the condensed lamellar phase into unilamellar DPPC+Zn2+ vesicles due to gel ↦ liquid-crystal phase transition in DNA+DPPC+20mM Zn2+ aggregates with the short fragmented salmon sperm DNA.


European Physical Journal Special Topic DPPC Repeat Distance DPPC Bilayer Lateral Segregation 
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  1. Y. Sato, N. Kumazawa, K. Yoshikawa, Y. Kurusu, Biosci. Biotechnol. Biochem. 69, 235 (2005) Google Scholar
  2. V.G. Budker, Y.A. Kazatchkov, L.P. Naumova, FEBS Lett. 95, 143 (1978) Google Scholar
  3. L. Vojčíková, P. Balgavý, Stud. Biophys. 125, 5 (1988) Google Scholar
  4. L. Vojčíková, E. Švajdlenka, P. Balgavý, Gen. Physiol. Biophys. 8, 399 (1989) Google Scholar
  5. R.S. Khusainova, K.A. Dawson, I. Rochev, A.V. Gorelov, G.R. Ivanitskii, Dokl. Akad. Nauk. 367, 553 (1999) Google Scholar
  6. D. Uhríková, M. Kovářová, P. Balgavý, Acta Facult. Pharm. Univ. Comenianae 51, 217 (2004) Google Scholar
  7. L. Vojčíková, P. Balgavý, Acta Facult. Pharm. Univ. Comenianae 52, 226 (2005) Google Scholar
  8. Y.S. Tarahovsky, A.V. Khusainova, A.V. Gorelov, T.I. Nicolaeva, A.A. Deev, A.K. Dawson, G.R. Ivanitsky, FEBS Lett. 390, 133 (1996) Google Scholar
  9. D. Uhríková, G. Rapp, P. Balgavý, in Challenges for Coordination Chemistry in the New Century, edited by M. Melník, A. Sirota (Bratislava, 2001), p. 219 Google Scholar
  10. O. Francescangeli, V. Stanic, L. Gobbi, P. Bruni, M. Iacussi, G. Tosi, S. Bernstorff, Phys. Rev. E 67, 011904 (2003) Google Scholar
  11. J. McManus, J.O. Rädler, K.A. Dawson, Langmuir 19, 9630 (2003) Google Scholar
  12. D. Uhríková, M. Hanulová, S.S. Funari, R.S. Khusainova, F. Šeršeň, P. Balgavý, Biochim. Biophys. Acta 1713, 15 (2005) Google Scholar
  13. D. Uhríková, A. Lengyel, M. Hanulová, S.S. Funari, P. Balgavý, Eur. Biophys. J. 36, 363 (2007) Google Scholar
  14. J.C. Shepherd, G. Büldt, Biochim. Biophys. Acta 514, 83 (1978) Google Scholar
  15. Y. Izumitani, J. Colloid Interface Sci. 166, 143 (1994) Google Scholar
  16. Ch. Altenbach, J. Seelig, Biochemistry 23, 3913 (1984) Google Scholar
  17. D. Huster, K. Arnold, Biophys. J. 75, 909 (1998) Google Scholar
  18. D. Uhríková, L. Benkovičová, M. Kovářová, P. Balgavý, in Book of abstract of the XXIV.Days of Medical Biophysics (Mozolov, Czech Republic, 2001), p. 65 Google Scholar
  19. T. Maniatis, E. Fritsch, J. Sambrook, in Molecular cloning, A laboratory manual, Cold Spring Harbor Laboratory, 1982 Google Scholar
  20. R.C. MacDonald, R.I. MacDonald, B.Ph.M. Menco, K. Takeshita, N.K. Subbarao, L.R. Hu, Biochim. Biophys. Acta 1061, 297 (2001) Google Scholar
  21. N. Roveri, A. Bigi, P.P. Castellani, E. Foresti, M. Marchini, R. Strocchi, Boll. Soc. Ital. Biol. Sper. 56, 953 (1980) Google Scholar
  22. N. Kučerka, D. Uhríková, J. Teixeira, P. Balgavý, Acta Facult. Pharm. Univ. Comenianae 50, 78 (2003) Google Scholar
  23. N. Albon, J.M. Sturtevant, Proc. Natl. Acad. Sci. USA 75, 2258 (1978) Google Scholar
  24. J. Stümpel, H.J. Eibl, A. Nicksch, Biochim. Biophys. Acta 727, 246 (1983) Google Scholar
  25. J.F. Nagle, S. Tristram-Nagle, Biochim. Biophys. Acta 1469, 159 (2000) Google Scholar
  26. W. Knoll, J. Haas, H.B. Sturhrmann, H.H. Fuldner, H. Vogel, E. Sackmann, J. Appl. Cryst. 14, 191 (1981) Google Scholar
  27. T. Nawroth, H. Conrad, K. Dose, Physica B 156 & 157, 477 (1989) Google Scholar
  28. P. Balgavý, M. Dubničková, D. Uhríková, S. Yaradaikin, M. Kiselev, V. Gordeliy, Acta Physica Slovaca 48, 509 (1998) Google Scholar
  29. H. Hauser, in Phospholipid Handbook, edited by G. Cevc (Marcel Dekker Inc., New York, 1993), p. 603 Google Scholar
  30. Y. Inoko, T. Yamaguchi, K. Furuya, T. Mitsui, Biochim. Biophys. Acta 413, 24 (1975) Google Scholar
  31. D. Uhríková, J. Teixeira, A. Lengyel, L. Almásy, P. Balgavý, Spectroscopy 21, 43 (2007) Google Scholar
  32. N. Kučerka, J.F. Nagle, S.E. Feller, P. Balgavý, Phys. Rev. E 69, 051903 (2004) Google Scholar
  33. D. Uhríková, N. Kučerka, J. Teixeira, V. Gordeliy, P. Balgavý, Chem. Phys. Lipids 155, 80 (2008) Google Scholar
  34. R. Bruinsma, J. Mashl, Europhys. Lett. 41, 165 (1998) Google Scholar
  35. D. Harries, S. May, W.M. Gelbart, A. Ben Shaul, Biophys. J. 75, 159 (1998) Google Scholar
  36. H. Binder, O. Zschornig, Chem. Phys. Lipids 115, 39 (2002) Google Scholar
  37. N. Gresh, J. Šponer, J. Phys. Chem. B 103, 11415 (1999) Google Scholar

Copyright information

© EDP Sciences and Springer 2009

Authors and Affiliations

  • D. Uhríková
    • 1
  • P. Pullmannová
    • 1
  • N. Kučerka
    • 1
    • 2
  • S. S. Funari
    • 3
  • J. Teixeira
    • 4
  • P. Balgavý
    • 1
  1. 1.Faculty of Pharmacy, Comenius UniversityBratislavaSlovakia
  2. 2.Canadian Neutron Beam Centre, National Research Council, Chalk RiverOntarioCanada
  3. 3.HASYLAB, DESYHamburgGermany
  4. 4.Laboratoire Léon Brillouin (CEA-CNRS), CEA SaclayGif-sur-Yvette CedexFrance

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