Advertisement

PS2.M: Looking for a potassium biosensor

  • Luca Bruni
  • Massimo Manghi
  • Simonetta CrociEmail author
Regular Article
  • 38 Downloads

Abstract.

DNA sequences with guanine repeats are able to fold as G-quadruplex (G4) structures. This is an alternative DNA conformation in which four guanines are arranged as a tetrad, the structural unit of G4; two or more stacked tetrads form a G4 structure. The hydrogen bonds characterizing G4 are called Hoogsten bonds but more interactions are involved in the G4 structure stabilization. For example, cations work as G4 stabilizers and their role is not restricted to the structural folding. They coordinate the guanines’ carbonilic oxygens located towards the hydrophobic channel of the G4 inner structure. This feature suggests that the G4 could work as a cation biosensor. Biological media are characterized by the simultaneous presence of K+ and Na+ exhibiting different affinities and thus promoting different topological arrangements in the folding solution. In this article we explore the possibility of using PS2.M, an 18 base long synthetic oligonucleotide, as a detector of K+ at concentrations in the range 0mM to 10mM. Our intent is therefore to study a biosensor that is made of the G4 sequence only, without intervention of other coupled molecules. As with most guanine-rich oligonucleotides, also PS2.M shows different structures depending on the folding conditions. In agreement with the literature data, our results outline the expected behavior and the key role of K+ and Na+ in promoting the folding, with Na+ promoting the antiparallel structure formation of PS2.M, even at high concentrations. On the other hand, if PS2.M is folded in 10mM to 100mM KCl solutions, the parallel conformation sets in becoming more and more relevant as concentration grows. In a complex solution of G4 in the presence of both K+ and Na+ ions, spectra display the coexistence of parallel, antiparallel and mixed type conformations. Results show that the CD spectra values at this wavelength can be diagrammed as a function of the K+ concentration to construct a biosensor calibration curve. In conclusion, given a Na+ concentration in the range 50mM to 80mM and K+ concentration in the range 0mM to 10mM, the measured CD signal at 263.6nm permits a K+ concentration measurement with a resolution of ∼ 1 mM.

References

  1. 1.
    S. Burge, G.N. Parkinson, P. Hazel, A.K. Todd, S. Neidle, Nucleic Acids Res. 34, 5402 (2006)CrossRefGoogle Scholar
  2. 2.
    S.K. Mishra, A. Tawani, A. Mishra, A. Kumar, Sci. Rep. 6, 38144 (2016)ADSCrossRefGoogle Scholar
  3. 3.
    B. Ivar, Biochem. Z. 26, 293 (1910)Google Scholar
  4. 4.
    M. Gellert, M.N. Lipsett, D.R. Davies, Proc. Natl. Acad. Sci. U.S.A. 48, 2013 (1962)ADSCrossRefGoogle Scholar
  5. 5.
    B. Ruttkay-Nedecky, J. Kudr, L. Nejdl, D. Maskova, R. Kizek, V. Adam, Molecules 18, 14760 (2013)CrossRefGoogle Scholar
  6. 6.
    C.E. Pearson, R.R. Sinden, Curr. Opin. Struct. Biol. 8, 321 (1998)CrossRefGoogle Scholar
  7. 7.
    G.N. Parkinson, M.P.H. Lee, S. Neidle, Nature 417, 876 (2002)ADSCrossRefGoogle Scholar
  8. 8.
    O. Doluca, J.M. Withers, V.V. Filichev, Chem. Rev. 113, 3044 (2013)CrossRefGoogle Scholar
  9. 9.
    N. Maizels, Nat. Struct. Mol. Biol. 13, 1055 (2006)CrossRefGoogle Scholar
  10. 10.
    N. Maizels, L.T. Gray, Plos Genet. 9, e1003468 (2013)CrossRefGoogle Scholar
  11. 11.
    R.D. Gray, J. Li, J.B. Chaires, J. Phys. Chem. B 113, 2676 (2009)CrossRefGoogle Scholar
  12. 12.
    G.N. Parkinson, R. Ghosh, S. Neidle, Biochemistry 46, 2390 (2007)CrossRefGoogle Scholar
  13. 13.
    P.R. Majhi, R.H. Shafer, Biopolymers 82, 558 (2006)CrossRefGoogle Scholar
  14. 14.
    D. Bhattacharyya, G. Mirihana Arachchilage, S. Basu, Front. Chem. 4, 38 (2016)CrossRefGoogle Scholar
  15. 15.
    W. Liu, H. Zhu, B. Zheng, S. Cheng, Y. Fu, W. Li, T.C. Lau, H. Liang, Nucleic Acids Res. 40, 4229 (2012)CrossRefGoogle Scholar
  16. 16.
    D.Z. Yang, K. Okamoto, Future Med. Chem. 2, 619 (2010)CrossRefGoogle Scholar
  17. 17.
    H.Z. He, D.S.H. Chan, C.H. Leung, D.L. Ma, Nucleic Acids Res. 41, 4345 (2013)CrossRefGoogle Scholar
  18. 18.
    N. Shahbazi, S. Hosseinkhani, K. Khajeh, B. Ranjbar, Biopolymers 107, e23028 (2017)CrossRefGoogle Scholar
  19. 19.
    P. Travascio, Y. Li, D. Sen, Chem. Biol. 5, 505 (1998)CrossRefGoogle Scholar
  20. 20.
    G. Wang, L. Chen, Y. Zhu, X. He, G. Xu, X. Zhang, Biosensors Bioelectron. 61, 410 (2014)CrossRefGoogle Scholar
  21. 21.
    D.M. Kong, L.L. Cai, J.H. Guo, J. Wu, H.X. Shen, Biopolymers 91, 331 (2009)CrossRefGoogle Scholar
  22. 22.
    X. Cheng, X. Liu, T. Bing, Z. Cao, D. Shangguan, Biochemistry 48, 7817 (2009)CrossRefGoogle Scholar
  23. 23.
    S. Croci, L. Bruni, S. Bussolati, M. Castaldo, M. Dondi, Cancer Cell Int. 11, 30 (2011)CrossRefGoogle Scholar
  24. 24.
    L. Bruni, A.A. Babarinde, I. Ortalli, S. Croci, Cancer Cell Int. 14, 77 (2014)CrossRefGoogle Scholar
  25. 25.
    S. Paramasivan, I. Rujan, P.H. Bolton, Methods 43, 324 (2007)CrossRefGoogle Scholar
  26. 26.
    V. Viglasky, L. Bauer, K. Tluckova, Biochemistry 49, 2110 (2010)CrossRefGoogle Scholar
  27. 27.
    A. Bugaut, S. Balasubramanian, Biochemistry 47, 689 (2008)CrossRefGoogle Scholar
  28. 28.
    P.C. Wei, Z.F. Wang, W.T. Lo, M.I. Su, J.Y. Shew, T.C. Chang, W.H. Lee, Nucleic Acids Res. 41, 1533 (2013)CrossRefGoogle Scholar
  29. 29.
    M. Lu, Q. Guo, N. Kallenbach, Biochemistry 32, 598 (1993)CrossRefGoogle Scholar
  30. 30.
    T. Li, E. Wang, S. Dong, Anal. Chem. 82, 7576 (2010)CrossRefGoogle Scholar
  31. 31.
    X. Sun, Q. Li, J. Xiang, L. Wang, X. Zhang, L. Lan, S. Xu, F. Yang, Y. Tang, Analyst 142, 3352 (2017)ADSCrossRefGoogle Scholar

Copyright information

© Società Italiana di Fisica and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Dipartimento di Medicina e ChirurgiaUniversità degli Studi di ParmaParmaItaly
  2. 2.Museo Storico della Fisica e Centro Studi e Ricerche Enrico FermiRomeItaly
  3. 3.Istituto Nazionale Biostrutture e BiosistemiRomeItaly

Personalised recommendations