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The white confocal

  • R. Th. Borlinghaus
Regular Article

Abstract

Modern biomedical research is currently dominated by imaging and measuring with optical microscopes. One branch of the microscopy technology is confocal microscopy. For correlation purposes, multiparameter fluorescence imaging is particularly of unique interest. This article is concerned with the spectral performance of the various modules in a confocal point-scanning microscope (“True Confocal System”), and how these modules have evolved to allow for tunability and flexibility in excitation and emission collection in multiple bands (channels). These modules are: light source, illumination modifier, beam splitter, emission filter, band separator and sensor. The final composition of modern technologies, some of them including acousto-optical devices, constitutes a system, that is no more restricted in terms of wavelength dependencies. It is therefore called a “white” confocal in analogy to physically white light, that has a constant energy distribution (spectrum) over the visible range (400nm-800nm).

Keywords

Beam Splitter Optical Section Beam Path United States Patent Emission Beam 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Società Italiana di Fisica and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • R. Th. Borlinghaus
    • 1
  1. 1.Leica MicrosystemsMannheimGermany

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