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A detailed guideline for the fabrication of single bacterial probes used for atomic force spectroscopy

  • Nicolas Thewes
  • Peter Loskill
  • Christian Spengler
  • Sebastian Hümbert
  • Markus Bischoff
  • Karin Jacobs
Tips and Tricks

Abstract.

The atomic force microscope (AFM) evolved as a standard device in modern microbiological research. However, its capability as a sophisticated force sensor is not used to its full capacity. The AFM turns into a unique tool for quantitative adhesion research in bacteriology by using “bacterial probes”. Thereby, bacterial probes are AFM cantilevers that provide a single bacterium or a cluster of bacteria as the contact-forming object. We present a step-by-step protocol for preparing bacterial probes, performing force spectroscopy experiments and processing force spectroscopy data. Additionally, we provide a general insight into the field of bacterial cell force spectroscopy.

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Keywords

Tips and Tricks 

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Copyright information

© EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Nicolas Thewes
    • 1
  • Peter Loskill
    • 1
  • Christian Spengler
    • 1
  • Sebastian Hümbert
    • 1
  • Markus Bischoff
    • 2
  • Karin Jacobs
    • 1
  1. 1.Experimental Physics, Campus E2 9Saarland UniversitySaarbrückenGermany
  2. 2.Institute of Medical Microbiology and HygieneSaarland UniversityHomburg/SaarGermany

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