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Charged particles interacting with a mixed supported lipid bilayer as a biomimetic pulmonary surfactant

  • B. Munteanu
  • F. Harb
  • J. P. Rieu
  • Y. Berthier
  • B. Tinland
  • A. -M. Trunfio-Sfarghiu
Regular Article

Abstract

This study shows the interactions of charged particles with mixed supported lipid bilayers (SLB) as biomimetic pulmonary surfactants. We tested two types of charged particles: positively charged and negatively charged particles. Two parameters were measured: adsorption density of particles on the SLB and the diffusion coefficient of lipids by FRAPP techniques as a measure of interaction strength between particles and lipids. We found that positively charged particles do not adsorb on the bilayer, probably due to the electrostatic repulsion between positively charged parts of the lipid head and the positive groups on the particle surface, therefore no variation in diffusion coefficient of lipid molecules was observed. On the contrary, the negatively charged particles, driven by electrostatic interactions are adsorbed onto the supported bilayer. The adsorption of negatively charged particles increases with the zeta-potential of the particle. Consecutively, the diffusion coefficient of lipids is reduced probably due to binding onto the lipid heads which slows down their Brownian motion. The results are directly relevant for understanding the interactions of particulate matter with pulmonary structures which could lead to pulmonary surfactant inhibition or deficiency causing severe respiratory distress or pathologies.

Graphical abstract

Keywords

Living systems: Biomimetic Systems 

References

  1. 1.
    M. Brauer, G. Hoek, P. Van Vliet, K. Meliefste, P.H. Fischer, A. Wijga, L.P. Koopman, H.J. Neijens, J. Gerritsen, M. Kerkhof, J. Heinrich, T. Bellander, B. Brunekreef, Am. J. Resp. Crit. Care Med. 166, 1092 (2002).Google Scholar
  2. 2.
    D. Dockery, C.A. Pope, X. Xu, J. Spengler, J. Ware, M. Fay, B. Ferris, F. Speizer, New Engl. J. Med. 329, 1753 (1993).CrossRefGoogle Scholar
  3. 3.
    A. Zanobetti, J. Schwartz, D.W. Dockery, Environ. Health Perspect. 108, 1071 (2000).CrossRefGoogle Scholar
  4. 4.
    E. Boldo, S. Medina, A. Le Tertre, F. Hurley, H.G. Mucke, F. Ballester, I. Aguilera, Eur. J. Epidemiol. 21, 449 (2006).CrossRefGoogle Scholar
  5. 5.
    P. Watkiss, S. Pye, M. Holland, Service Contract for carrying out cost-benefit analysis of air quality related issues, in particular in the clean air for Europe (CAFE) programme (European Commission DG Environment, 2003).Google Scholar
  6. 6.
    European Commission, Characterisation of Exhaust Particulate Emissions from Road Vehicles (October, 2004). .Google Scholar
  7. 7.
    J.W. Goodwin, A.G. Salway, C.J. Dore, T.P. Murrells, N.R. Passant, K.R. King, P.J. Coleman, M.M. Hobson, S.T. Pye, J.D. Watterson, K.E. Haigh, C.M. Conolly, UK emissions of air pollutants 1970-2000 (2010).Google Scholar
  8. 8.
    J. Denape, Y. Berthier, L. Vincent, in Fundamentals of Tribology and Bridging the Gap Between the Macro- and Micro/Nanoscales, NATO Science Series, Vol. 10 (2001) pp. 393--411.Google Scholar
  9. 9.
    B. Lebeau, Pneumologie, Vol. 1 (Ellipses Éd., Paris, 1989).Google Scholar
  10. 10.
    T. Kanishtha, R. Banerjee, C. Venkataraman, Environ. Toxicol. Pharmacol. 22, 325 (2006).CrossRefGoogle Scholar
  11. 11.
    B.A. Hills, British J. Anaesth. 65, 13 (1990).CrossRefGoogle Scholar
  12. 12.
    L.A. Creuwels, L.M. van Golde, H.P. Haagsman, Lung 175, 1 (1997).CrossRefGoogle Scholar
  13. 13.
    J. Pérez-Gil, Biochim. Biophys. Acta 1778, 1676 (2008).CrossRefGoogle Scholar
  14. 14.
    A.G. Serrano, J. Pérez-Gil, Chem. Phys. Lipids 141, 105 (2006).Google Scholar
  15. 15.
    J. Goerke, Biochim. Biophys. Acta 1408, 79 (1998).CrossRefGoogle Scholar
  16. 16.
    R. Veldhuizen, K. Nag, S. Orgeig, F. Possmayer, Biochim. Biophys. Acta 1408, 90 (1998).CrossRefGoogle Scholar
  17. 17.
    E. Veldhuizen, H. Haagsman, Biochim. Biophys. Acta 1467, 255 (2000).CrossRefGoogle Scholar
  18. 18.
    R. Wustneck, J. Perez-Gil, N. Wustneck, A. Cruz, V.B. Fainerman, U. Pison, Adv. Colloid Interface Sci. 117, 33 (2005).CrossRefGoogle Scholar
  19. 19.
    J. Perez-Gil, K.M. Keough, Biochim. Biophys. Acta 1408, 203 (1998).CrossRefGoogle Scholar
  20. 20.
    J.C. Watkins, Biochim. Biophys. Acta 152, 239 (1968).CrossRefGoogle Scholar
  21. 21.
    R.K. Harishchandra, M. Saleem M, H.J. Galla, J. R. Soc. Interface 7, S15 (2010).CrossRefGoogle Scholar
  22. 22.
    E. Guzmán, L. Liggieri, E. Santini, M. Ferrari, F. Ravera, Colloids Surf. A: Physicochem. Engin. Asp. 413, 174 (2012).CrossRefGoogle Scholar
  23. 23.
    E. Guzmán, L. Liggieri, E. Santini, M. Ferrari, F. Ravera, J. Phys. Chem. C 115, 21715 (2011).CrossRefGoogle Scholar
  24. 24.
    F. Harb, A. Simon, B. Tinland, Eur. Phys. J. E 36, 140 (2013).CrossRefGoogle Scholar
  25. 25.
    A.P. Girard-Egrot, L.J. Blum, Nanobiotechnol. Biomim. Membr. XII, 23 (2007).ADSCrossRefGoogle Scholar
  26. 26.
    L.K. Tamm, Biochemistry 27, 1450 (1988).CrossRefGoogle Scholar
  27. 27.
    V. Gerkea, S.E. Moss, Biochim. Biophys. Acta 1357, 129 (1997).CrossRefGoogle Scholar
  28. 28.
    W. Stillwell, An Introduction to Biological Membranes: From Bilayers to Rafts (Academic Press, Elsevier, 2013).Google Scholar
  29. 29.
    L. Yang, G. Ning, J. Phys. Chem. B 114, 2749 (2010).Google Scholar
  30. 30.
    B. Wanga, L. Zhangb, S. Baea, S. Granick, Proc. Natl. Acad Sci. U.S.A. 105, 18171 (2008).ADSCrossRefGoogle Scholar
  31. 31.
    L. Zhang, S. Granick, Nano Lett. 6, 694 (2006).ADSCrossRefGoogle Scholar
  32. 32.
    M.P. Goertz, N. Goyal, B.C. Bunker, B.A. Montañ, J. Colloid Interface Sci. 358, 635 (2011).CrossRefGoogle Scholar

Copyright information

© EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • B. Munteanu
    • 1
  • F. Harb
    • 2
    • 4
  • J. P. Rieu
    • 3
  • Y. Berthier
    • 1
  • B. Tinland
    • 2
  • A. -M. Trunfio-Sfarghiu
    • 1
  1. 1.CNRS, INSA de Lyon, LaMCoS, UMR5259Université de LyonLyonFrance
  2. 2.CNRS, CINaM, UMR7325Université Aix-MarseilleMarseilleFrance
  3. 3.CNRS, UCBL, ILM, UMR5586Université de LyonLyonFrance
  4. 4.Department of Biology; Faculty of Sciences - Section IILebanese UniversityBeirutLebanon

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