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Structural modulation of the biological activity of gold nanoparticles functionalized with a carbonic anhydrase inhibitor

  • Francesca Bellissima
  • Fabrizio Carta
  • Alessio Innocenti
  • Andrea Scozzafava
  • Piero Baglioni
  • Claudiu T. Supuran
  • Debora Berti
Regular Article

Abstract

Gold nanoparticles (AuNPs) have gained attention for their potential and application in different fields, e.g. nanomedicine. This study explores the surface functionalization of AuNP with inhibitors of carbonic anhydrases (CAs, EC 4.2.1.1). Some CA transmembrane isoforms have been recognized as therapeutic targets for the treatment of hypoxic tumors. Embedding a CA inhibitory function onto a nanosized unit has been proved to enable selective targeting of transmembrane isoforms. We report the preparation in aqueous media, the characterization and CA inhibition tests of AuNPs coated with a sulfonamide (SA) derivative, already known for its inhibitory activity toward CAs. The physico-chemical characterization of SA-coated AuNPs was performed with a combination of scattering and spectroscopic techniques. We detect a threshold effect of the SA concentration on the final hydrodynamic and core sizes of the capped nanoparticles and on their stability over aggregation. These modified nanoparticles were assayed for inhibition of some CA transmembrane isoforms (CA IX and XII) as well as of two cytosolic isoforms (CA I and II), and show interesting inhibitory efficiency in the submicromolar range and some selectivity for transmembrane isoforms.

Graphical abstract

Keywords

Soft Matter: Colloids and Nanoparticles 

List of abbreviations

AuNP

gold nanoparticles

CA

carbonic anhydrase

SA

sulfonamide

CAI

carbonic anhydrase inhibitor

DMSO

dimethylsulfoxide

DLS

dynamic light scattering

SAXS

small angle X-ray scattering

ATR-FTIR

attenuated total reflection Fourier-transformed infrared spectroscopy

Na3Ct

trisodium citrate dihydrate

ICP-AES

inductively coupled plasma-atomic emission spectroscopy

TGA

thermogravimetric analysis

SLD

scattering length density

TEM

transmission electron microscopy

SPR

surface plasmon resonance

DLVO

Derjaguin, Landau, Verwey, Overbeek

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Copyright information

© EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Francesca Bellissima
    • 1
  • Fabrizio Carta
    • 1
  • Alessio Innocenti
    • 1
  • Andrea Scozzafava
    • 1
  • Piero Baglioni
    • 1
  • Claudiu T. Supuran
    • 1
  • Debora Berti
    • 1
  1. 1.Department of Chemistry and CSGIUniversity of FlorenceFlorenceItaly

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