Advertisement

SAXS study on myoglobin embedded in amorphous saccharide matrices

  • S. GiuffridaEmail author
  • M. Panzica
  • F. M. Giordano
  • A. Longo
Regular Article
Part of the following topical collections:
  1. Topical Issue on the Physics of Glasses

Abstract

We report on Small Angle X-ray Scattering (SAXS) measurements performed on samples of carboxy-myoglobin and met-myoglobin embedded in low hydrated matrices of four different saccharides (trehalose, sucrose, maltose and lactose). Results confirm the already reported occurrence of inhomogeneities, which are not peculiar of trehalose samples, but appear also in maltose and lactose, and in some cases also sucrose, being dependent on the sample hydration and on the presence of sodium dithionite. This behaviour confirms our previous interpretation about the nature of the inhomogeneities, and prompt it as a possible general behaviour for highly concentrated sugar matrices.

Keywords

Lactose Maltose Trehalose Disaccharide Dithionite 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    L.M. Crowe, Comp. Biochem. Physiol. A 132, 505 (2002).CrossRefGoogle Scholar
  2. 2.
    J.H. Crowe, Adv. Exp. Med. Biol. 594, 143 (2007).CrossRefGoogle Scholar
  3. 3.
    C.L.A. Paiva, A.D. Panek, Biotechnol. Annu. Rev. 2, 293 (1996).CrossRefGoogle Scholar
  4. 4.
    J.F. Carpenter, J.H. Crowe, Biochemistry 28, 3916 (1989).CrossRefGoogle Scholar
  5. 5.
    P.S. Belton, A.M. Gil, Biopolymers 34, 957 (1994).CrossRefGoogle Scholar
  6. 6.
    J.G. Sampedro, S. Uribe, Mol. Cell Biochem. 256, 319 (2004).CrossRefGoogle Scholar
  7. 7.
    A. Hédoux, J.-F. Willart, R. Ionov, F. Affouard, Y. Guinet, L. Paccou, A. Lerbret, M. Descamps, J. Phys. Chem. B 110, 22886 (2006).CrossRefGoogle Scholar
  8. 8.
    S. Magazù, G. Maisano, F. Migliardo, C. Mondelli, Biophys. J. 86, 3241 (2004).ADSCrossRefGoogle Scholar
  9. 9.
    F. Sussich, C. Skopec, J. Brady, A. Cesàro, Carbohydr. Res. 334, 165 (2001).CrossRefGoogle Scholar
  10. 10.
    A. Cesàro, Nat. Mater. 5, 593 (2006).CrossRefGoogle Scholar
  11. 11.
    S.J. Hagen, J. Hofrichter, W.A. Eaton, Science 269, 959 (1995).ADSCrossRefGoogle Scholar
  12. 12.
    L. Cordone, G. Cottone, S. Giuffrida, G. Palazzo, G. Venturoli, C. Viappiani, BBA-Proteins Proteomics 1749, 252 (2005).CrossRefGoogle Scholar
  13. 13.
    S. Giuffrida, G. Cottone, L. Cordone, Biophys. J. 91, 968 (2006).ADSCrossRefGoogle Scholar
  14. 14.
    L. Cordone, G. Cottone, S. Giuffrida, J. Phys.: Condens. Matter 19, 205110 (2007).ADSCrossRefGoogle Scholar
  15. 15.
    L. Cordone, G. Cottone, S. Giuffrida, F. Librizzi, Chem. Phys. 345, 275 (2008).ADSCrossRefGoogle Scholar
  16. 16.
    G. Cottone, L. Cordone, G. Ciccotti, Biophys. J. 80, 931 (2001).ADSCrossRefGoogle Scholar
  17. 17.
    F. Francia, G. Palazzo, A. Mallardi, L. Cordone, G. Venturoli, Biochim. Biophys. Acta Bioenerg. 1658, 50 (2004).CrossRefGoogle Scholar
  18. 18.
    G. Cottone, G. Ciccotti, L. Cordone, J. Chem. Phys. 117, 9862 (2002).ADSCrossRefGoogle Scholar
  19. 19.
    G. Cottone, J. Phys. Chem. B 111, 3563 (2007).CrossRefGoogle Scholar
  20. 20.
    G. Bellavia, L. Cordone, A. Cupane, J. Therm. Anal. Calorim. 95, 699 (2009).CrossRefGoogle Scholar
  21. 21.
    G. Bellavia, G. Cottone, S. Giuffrida, A. Cupane, L. Cordone, J. Phys. Chem. B 113, 11543 (2009).CrossRefGoogle Scholar
  22. 22.
    U. Heugen, G. Schwaab, E. Bründermann, M. Heyden, X. Yu, D.M. Leitner, M. Havenith, Proc. Natl. Acad. Sci. U.S.A. 103, 12301 (2006).ADSCrossRefGoogle Scholar
  23. 23.
    M. Heyden, E. Bründermann, U. Heugen, G. Niehues, D.M. Leitner, M. Havenith, J. Am. Chem. Soc. 130, 5773 (2008).CrossRefGoogle Scholar
  24. 24.
    S. Giuffrida, G. Cottone, F. Librizzi, L. Cordone, J. Phys. Chem. B 107, 13211 (2003).CrossRefGoogle Scholar
  25. 25.
    S. Giuffrida, G. Cottone, L. Cordone, J. Phys. Chem. B 108, 15415 (2004).CrossRefGoogle Scholar
  26. 26.
    D. Eisenberg, W. Kauzmann, The Structure and Properties of Water (Oxford University Press, London, 1969).Google Scholar
  27. 27.
    S. Giuffrida, G. Cottone, E. Vitrano, L. Cordone, J. Non-Cryst. Solids 357, 677 (2011).ADSCrossRefGoogle Scholar
  28. 28.
    A. Longo, S. Giuffrida, G. Cottone, L. Cordone, Phys. Chem. Chem. Phys. 12, 6852 (2010).CrossRefGoogle Scholar
  29. 29.
    L.A. Feigin, D.I. Svergun, Structure Analysis by Small Angle X-Ray and Neutron Scattering (Plenum Press, New York, 1987).Google Scholar
  30. 30.
    P. Debye, H.R. Anderson, H. Brumberger, J. Appl. Phys. 28, 679 (1957).ADSCrossRefGoogle Scholar
  31. 31.
    H. Brumberger, Modern Aspect of Small-Angle Scattering (Kluwer Academic Publishers, Dordrecht, 1993).Google Scholar
  32. 32.
    G.M. Brown, H.A. Levy, Acta Crystallogr. B 29, 790 (1973).CrossRefGoogle Scholar
  33. 33.
    K. Ibel, H.B. Stuhrmann, J. Mol. Biol. 93, 255 (1975).CrossRefGoogle Scholar
  34. 34.
    J.S. Pedersen, Adv. Colloid Interface Sci. 70, 171 (1997).CrossRefGoogle Scholar
  35. 35.
    Y.H. Lam, R. Bustami, T. Phan, H.K. Chan, F. Separovic, J. Pharm. Sci. 91, 943 (2002).CrossRefGoogle Scholar
  36. 36.
    A.M. Lammert, S.J. Schmidt, G.A. Day, Food Chem. 61, 139 (1998).CrossRefGoogle Scholar
  37. 37.
    A. Lerbret, P. Bordat, F. Affouard, M. Descamps, F. Migliardo, J. Phys. Chem. B 109, 11046 (2005).CrossRefGoogle Scholar
  38. 38.
    D. Kilburn, S. Townrow, V. Meunier, R. Richardson, A. Alam, J. Ubbink, Nat. Mater. 5, 632 (2006).ADSCrossRefGoogle Scholar

Copyright information

© EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  • S. Giuffrida
    • 1
    Email author
  • M. Panzica
    • 1
  • F. M. Giordano
    • 2
  • A. Longo
    • 2
  1. 1.Dipartimento di FisicaUniversità degli Studi di PalermoPalermoItaly
  2. 2.Istituto per lo Studio dei Materiali NanostrutturatiCNRPalermoItaly

Personalised recommendations