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The European Physical Journal E

, Volume 23, Issue 3, pp 247–254 | Cite as

Influence of cholesterol on the bilayer properties of monounsaturated phosphatidylcholine unilamellar vesicles

  • N. Kučerka
  • J. Pencer
  • M. -P. Nieh
  • J. Katsaras
Regular Article

Abstract.

The influence of cholesterol on the structure of unilamellar-vesicle (ULV) phospholipid bilayers is studied using small-angle neutron scattering. ULVs made up of short-, mid- and long-chain monounsaturated phospholipids (diCn :1PC, n = 14 , 18, 22, respectively) are examined over a range (0-45mol %) of cholesterol concentrations. Cholesterol's effect on bilayer structure is characterized through changes to the lipid's transmembrane thickness, lateral area and headgroup hydration. For all three lipids, analysis of the experimental data shows that the addition of cholesterol results in a monotonic increase of these parameters. In the case of the short- and mid-chain lipids, this is an expected result, however, such a finding was unexpected for the long-chain lipid. This implies that cholesterol has a pronounced effect on the lipid's hydrocarbon chain organization.

PACS.

61.12.Ex Neutron scattering (including small-angle scattering) 87.14.Cc Lipids 87.16.Dg Membranes, bilayers, and vesicles 87.68.+z Biomaterials and biological interfaces 

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Copyright information

© EDP Sciences, Società Italiana di Fisica and Springer-Verlag 2007

Authors and Affiliations

  • N. Kučerka
    • 1
    • 2
  • J. Pencer
    • 1
  • M. -P. Nieh
    • 1
  • J. Katsaras
    • 1
    • 3
    • 4
  1. 1.Canadian Neutron Beam CentreNational Research CouncilChalk River, OntarioCanada
  2. 2.Department of Physical Chemistry of Drugs, Faculty of PharmacyComenius UniversityBratislavaSlovakia
  3. 3.Guelph-Waterloo Physics Institute and Biophysics Interdepartmental GroupUniversity of GuelphGuelph, OntarioCanada
  4. 4.Department of PhysicsBrock UniversitySt. Catharines, OntarioCanada

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