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The European Physical Journal D

, Volume 60, Issue 1, pp 157–161 | Cite as

Spatio-temporal analysis of DNA damage repair using the X-ray microbeam

  • G. SchettinoEmail author
  • M. Ghita
  • K. M. Prise
Topical issue on Molecular level assessments of radiation biodamage

Abstract

Cellular response to radiation damage is made by a complex network of pathways and feedback loops whose spatiotemporal organization is still unclear despite its decisive role in determining the fate of the damaged cell. The single-cell approach and the high spatial resolution offered by microbeams provide the perfect tool to study and quantify the dynamic processes associated with the induction and repair of DNA damage. The soft X-ray microbeam has been used to follow the development of radiation induced foci in live cells by monitoring their size and intensity as a function of dose and time using yellow fluorescent protein (YFP) tagging techniques. Preliminary data indicate a delayed and linear rising of the intensity signal indicating a slow kinetic for the accumulation of DNA repair protein 53BP1. A slow and limited foci diffusion has also been observed. Further investigations are required to assess whatever such diffusion is consistent with a random walk pattern or if it is the result of a more structured lesion processing phenomenon. In conclusion, our data indicates that the use of microbeams coupled to live cell microscopy represent a sophisticated approach for visualizing and quantifying the dynamics changes of DNA proteins at the damaged sites.

Keywords

Yellow Fluorescent Protein Focus Intensity Double Strand Break Lanthanum Hexaboride Microbeam Irradiation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Center for Cancer Research and Cell BiologyQueen’s University BelfastNorthern IrelandUK

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