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The European Physical Journal D

, Volume 54, Issue 2, pp 195–204 | Cite as

DC discharges in atmospheric air for bio-decontamination – spectroscopic methods for mechanism identification

  • Z. MachalaEmail author
  • I. Jedlovský
  • L. Chládeková
  • B. Pongrác
  • D. Giertl
  • M. Janda
  • L. Šikurová
  • P. Polčic
Topical issue: 23rd Symposium on Plasma Physics and Technology

Abstract

Three types of DC electrical discharges in atmospheric air (streamer corona, transient spark and glow discharge) were tested for bio-decontamination of bacteria and yeasts in water solution, and spores on surfaces. Static vs. flowing treatment of contaminated water were compared, in the latter the flowing water either covered the grounded electrode or passed through the high voltage needle electrode. The bacteria were killed most efficiently in the flowing regime by transient spark. Streamer corona was efficient when the treated medium flew through the active corona region. The spores on plastic foil and paper surfaces were successfully inactivated by negative corona. The microbes were handled and their population evaluated by standard microbiology cultivation procedures. The emission spectroscopy of the discharges and TBARS (thiobarbituric acid reactive substances) absorption spectrometric detection of the products of lipid peroxidation of bacterial cell membranes indicated a major role of radicals and reactive oxygen species among the bio-decontamination mechanisms.

PACS

52.80.Hc Glow; corona 52.80.Tn Other gas discharges 52.70.Kz Optical 87.64.-t Spectroscopic and microscopic techniques in biophysics and medical physics 

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Copyright information

© EDP Sciences, SIF, Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Z. Machala
    • 1
    Email author
  • I. Jedlovský
    • 2
  • L. Chládeková
    • 2
  • B. Pongrác
    • 1
  • D. Giertl
    • 2
  • M. Janda
    • 1
  • L. Šikurová
    • 2
  • P. Polčic
    • 3
  1. 1.Division of Environmental PhysicsFaculty of Mathematics, Physics and Informatics, Comenius UniversityBratislavaSlovakia
  2. 2.Division of Biomedical PhysicsFaculty of Mathematics, Physics and Informatics, Comenius UniversityBratislavaSlovakia
  3. 3.Faculty of Natural Sciences, Comenius UniversityBratislavaSlovakia

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