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Microarray technology for the study of DNA damage by low-energy electrons

  • T. SolomunEmail author
  • C. Hultschig
  • E. Illenberger
Processes in Biomolecules

Abstract.

The damage induced to a model DNA (dT25) immobilized on a gold surface by the interaction of low-energy (1 eV) electrons was studied by means of microarray technology. High quality single-stranded DNA arrays were hybridized with a dye-marked complementary strand after irradiation with electrons and the normalized fluorescence data were used to quantify the DNA damage. The data clearly show the sensitivity of the method. A significant loss of genetic information was already observed at dose as low as few hundred of electrons per immobilized oligonucleotide. The results imply that single stranded DNA and RNA are appreciably more sensitive to radiation and the attack of secondary electrons during replication, transcription or translation stages than the current radiation damage models envisage.

Keywords

Genetic Information Secondary Electron Quantum Computing Radiation Damage Damage Model 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© EDP Sciences/Società Italiana di Fisica/Springer-Verlag 2005

Authors and Affiliations

  1. 1.Free University Berlin, Institute of ChemistryBerlinGermany
  2. 2.Department of Vertebrate GenomicsMax Planck Institute for Molecular GeneticsBerlinGermany

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