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Calcium Sparks in Cardiac Pacemaker Cells at Different Temperatures in silico

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Abstract

Temperature effects play an important role in the functioning of the cardiac cell. In this work we simulated the influence of temperature on calcium dynamics properties such as spontaneous calcium release frequency and amplitude while taking into account the experimental data on the response of various protein complexes to the temperature shift. We investigated temperature effects of protein complexes involved in calcium dynamics: calsequestrin and sarco-endoplasmic reticulum ATPase. The contribution of each of them to the temperature effect was explored. Furthermore, we introduced a novel approach of modelling the ryanodine receptors (RyRs) temperature effects and the contribution of RyRs thermal fluctuations to the whole cell temperature dependence was evaluated. The main result of our research is a generalization of the facts of the temperature influence on various calcium cycling proteins in the cardiac cell. Our main assumption is that calsequestrin makes the main contribution to the increase of calcium release frequency and in the increase of calcium concentration in sarcoplasmic reticulum.

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Abbreviations

CQ:

casequestrin

CCP:

calcium cycling protein

CM:

calmodulin

CRU:

calcium release unit

ECM:

electron-conformational model

jSR:

junctional SR

LCC:

L-type calcium channels

nSR:

SR network

RU:

release unit

RyR:

ryanodine receptor

SERCA:

sarcoplasmic reticulum Ca2+ ATPase

SR:

sarcoplasmic reticulum

SS:

subspace

TC:

troponin C

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Authors and Affiliations

  1. Institute of Immunology and Physiology, Ural Branch of Russian Academy of Sciences, Yekaterinburg, Russia

    A. M. Ryvkin

  2. Ural Fedreral University, Yekaterinburg, Russia

    A. M. Ryvkin & N. S. Markov

  3. Ural State Medical University, Yekaterinburg, Russia

    N. S. Markov

  4. Lawrenceville School, Lawrenceville, NJ, USA

    V. Yudenko

Authors
  1. A. M. Ryvkin
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  2. N. S. Markov
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  3. V. Yudenko
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Contributions

A.R. contributed to the conception of the study, literature review, data acquisition, data processing and analysis, software development, drafting the manuscript, editing the final version of manuscript. N.M. contributed to the conception of the study, software development, data analysis, editing the final version of manuscript. V.Y. contributed to computer experiments, data processing and analysis, software development.

Corresponding author

Correspondence to A. M. Ryvkin.

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The authors declare that they have no conflicts of interest.

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Translated by A. Polyanovsky

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Ryvkin, A.M., Markov, N.S. & Yudenko, V. Calcium Sparks in Cardiac Pacemaker Cells at Different Temperatures in silico. J Evol Biochem Phys 58 (Suppl 1), S125–S133 (2022). https://doi.org/10.1134/S0022093022070134

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