Efficiency of olfactory transport of manganese (II) oxide nanoparticles with single or multiple intranasal administration
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In experiments in which nano-sized metal oxide particles are multiply administered through inhalation, the absence of a material correlation between the number of they are administered and the metal concentration in olfactory bulbs (OBs) is demonstrated. This circumstance raises the question about a possible decrease in the efficiency of the capture of solid particles by the olfactory epithelium when they are repeatedly introduced in the nasal cavity. In this work, the efficiency of the nasal transport of magneto-contrasting nanoparticles during single and multiple intranasal administration is compared and their effect on the morphofunctional characteristics of the olfactory system is estimated. According to the data obtained, the accumulation of MnO-NP in the OBs of mice decreases during their repeated intranasal application. In addition, the decrease in the efficiency of the olfactory transport (observed during the multiple introduction of MnONP) partially recovers during the intranasal application of a mucolytic agent (0.01 M N-acetyl-L-cysteine). Moreover, the concentration of particles in OBs was proportional to the volume of this structure, which particularly depends on the number of synaptic contacts between the OBs and the olfactory epithelium. It should be noted that the olfactory epithelium’s thickness decreases during the multiple introduction of MnO-NP in mice. Thus, the efficiency of the olfactory transport of nanoparticles from the nasal cavity to the brain decreases during the multiple intranasal introduction of MnO-NP; this is combined with an increase in the mucosal layer’s viscosity and a decrease in the number of synaptic contacts between the OBs and the olfactory epithelium. The results obtained indicate the presence of the natural protection mechanisms of the olfactory epithelium against the penetration of pathogens and xenobiotics and allows us to formulate specific practical recommendations concerning the intranasal application of medications.
Keywordsnanoparticles intranasal introduction magnetic resonance imaging nasal transport
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- Bartsch, W.G.K.G., Sponer, G., Dietmann, K., and Fuchs, G., Acute toxicity of various solvents in the mouse and rat. LD50 of ethanol, diethylacetamide, dimethylformamide, dimethylsulfoxide, glycerine, N-me-thylpyrrolidone, polyethylene glycol 400, 1, 2-propanediol and tween 20, Arzneimittel-Forschung, 1975, vol. 26, no. 8, pp. 1581–1583.Google Scholar
- Calderón-Garcidueñas, L., Franco-Lira, M., Mora-Tiscareno, A., Medina-Cortina, H., Torres-Jardon, R., and Kavanaugh, M., Early Alzheimer’s and Parkinson’s disease pathology in urban children: Friend versus foe responses–it is time to face the evidence, BioMed Res. Int., 2013, vol. 2013, p. 161687.CrossRefPubMedPubMedCentralGoogle Scholar
- Elder, A., Gelein, R., Silva, V., Feikert, T., Opanashuk, L., Carter, J., Potter, R., Maynard, A., Ito, Y., Finkelstein, J., and Oberdorster, G., Translocation of inhaled ultrafine manganese oxide particles to the central nervous system, Environ. Health Perspect., 2006, vol. 114, no. 8, pp. 1172–1178.CrossRefPubMedPubMedCentralGoogle Scholar
- Hurtt, M.E., Thomas, D.A., Working, P.K., Monticello, T.M., and Morgan, K.T., Degeneration and regeneration of the olfactory epithelium following inhalation exposure to methyl bromide: Pathology, cell kinetics, and olfactory function, Toxicol. Appl. Pharmacol., 1988, vol. 94, no. 2, pp. 311–328.CrossRefPubMedGoogle Scholar
- Kitson, C., Angel, B., Judd, D., Rothery, S., Severs, N., Dewar, A., Huang, L., Wadsworth, S., Cheng, S., and Geddes, D., The extra-and intracellular barriers to lipid and adenovirus-mediated pulmonary gene transfer in native sheep airway epithelium, Gene Ther., 1999, vol. 6, no. 4, pp. 534–546.CrossRefPubMedGoogle Scholar
- Moshkin, M., Petrovski, D., Akulov, A., Romashchenko, A., Gerlinskaya, L., Ganimedov, V., Muchnaya, M., Sadovsky, A., Koptyug, I., and Savelov, A., Nasal aerodynamics protects brain and lung from inhaled dust in subterranean diggers, Ellobius talpinus, Proc. R. Soc. B: Biol. Sci., 2014, vol. 281, nos. 1792, p. 20140919.CrossRefGoogle Scholar
- Wang, J., Liu, Y., Jiao, F., Lao, F., Li, W., Gu, Y., Li, Y., Ge, C., Zhou, G., Li, B., Zhao, Y., Chai, Z., and Chen, C., Time-dependent translocation and potential impairment on central nervous system by intranasally instilled TiO2 nanoparticles, Toxicology, 2008, vol. 254, nos. 1–2, pp. 82–90.CrossRefPubMedGoogle Scholar