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Mapping of the loci controlling the resistance to Pyrenophora teres f. teres and Cochliobolus sativus in two double haploid barley populations

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Russian Journal of Genetics: Applied Research

Abstract

Net blotch of barley (Hordeum vulgare L.), caused by Pyrenophora teres f. teres, and spot blotch, caused by Cochliobolus sativus, are the most widespread and harmful diseases in the geographic range of the crop. Barley breeding for resistance to these diseases should employ large genetic diversity. The 11_11067 SNP marker was revealed on chromosome 6H position 58 cM in the double haploid (DH) population A developed by crossing the Ethiopian accession k-23874, highly resistant to P. teres f. teres, to the susceptible Pirkka cultivar. It was reliably (p < 0.05) associated with resistance to three P. teres f. teres isolates. In population B (Zernogradsky 813 (MR to C. sativus) Ranniy 1 (MR to P. teres f. teres), 11 QTL controlling resistance to 12 P. teres f. teres isolates were found on all barley chromosomes and 14 QTL for resistance to 12 C. sativus isolates, on all chromosomes except for 4H. For both pathogens, the revealed QTL were shown to be isolate-specific. The majority of the loci detected were mapped in the same intervals between SNP markers where QTL controlling resistance to P. teres f. teres and C. sativus had been found by other scientists. Four novel QTL controlling resistance to P. teres f. teres were found on chromosomes 1H, 4H, and 5H. Five novel QTL associated with resistance to C. sativus were found on chromosomes 2H, 3H, 5H, and 6H in DH population B.

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Correspondence to O. S. Afanasenko.

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Original Russian Text © O.S. Afanasenko, A.V. Koziakov, P.E. Hedlay, N.M. Lashina, A.V. Anisimova, O. Manninen, M. Jalli, E.K. Potokina, 2014, published in Vavilovskii Zhurnal Genetiki i Selektsii, 2014, Vol. 18, No. 4/1, pp. 751–764.

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Afanasenko, O.S., Koziakov, A.V., Hedlay, P.E. et al. Mapping of the loci controlling the resistance to Pyrenophora teres f. teres and Cochliobolus sativus in two double haploid barley populations. Russ J Genet Appl Res 5, 242–253 (2015). https://doi.org/10.1134/S2079059715030028

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