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The spermatogenous function in the BALB/cLac and DD/He murine inbred lines and their F1 reciprocal crosses

  • M. A. Kleshchev
  • A. V. Osadchuk
  • L. V. Osadchuk
Article
  • 13 Downloads

Abstract

Previously, it was determined that the murine inbred line DD/He has the lowest fraction of mobile and morphologically anomalous spermatozoa as compared with 13 other studied lines. In contrast, the BALB/cLac line is characterized by the highest fraction of mobile and anomalous spermatozoa. It was suggested that a decreased fraction of mobile spermatozoa in the murine DD/He line, as well as a high fraction of anomalous spermatozoa in BALB/cLac are caused by mutations in Y-chromosome genes. In order to test this hypothesis, the mobility and morphology of spermatozoa, as well as the amount of spermatozoa in the caudal epididymis, as well as the weight of the body and testes, were studied in F1 males of DD/He × BALB/cLac and BALB/cLac × DD/He reciprocal crosses. The heterosis effects for all studied traits (except the fraction of mobile spermatozoa) were determined. An influence of parental effects on the fraction of atypical spermatozoa and on the weight of testes was detected, which can indicate the involvement of Y-chromosome gene (genes) in the genetic control if these traits. Maternal effects on the fraction of mobile spermatozoa were found.

Keywords

murine inbred lines spermatogenesis reciprocal crossings 

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References

  1. Adham, I.M., Eck, T.J., Mierau, K., et al., Reduction of spermatogenesis but not fertility in Creb3l4-deficient mice, Mol. Cell. Biol., 2005, vol. 25, pp. 7657–7664.PubMedCrossRefGoogle Scholar
  2. Chen, J.C., Molecular mechanisms of polyploidy and hybrid vigor, Trends Plant Sci., 2010, vol. 15, pp. 1–28.CrossRefGoogle Scholar
  3. Daev, E.V. and Dukel’skaya, A.V., The female pheromone 2,5-dimethylpyrazine induces sperm-head abnormalities in male CBA mice, Russ. J. Genet., 2003, vol. 39, no. 7, pp. 811–815.CrossRefGoogle Scholar
  4. Delbridge, M.L. and Graves, J.M., Mammalian Y chromosome evolution and the male-specific functions of Y chromosome-borne genes, Rev. Reprod., 1999, vol. 4, pp. 101–109.PubMedCrossRefGoogle Scholar
  5. Guzick, D., Overstreet, J., and Factor-Litvak, P., Sperm morphology, motility, and concentration in fertile and infertile men, N. Engl. J. Med., 2001, vol. 345, p. 1388.PubMedCrossRefGoogle Scholar
  6. Hannon, R.M., Meek, H.T., Acosta, W., and Maciel, R.C., Sex-specific heterosis in line crosses of mice selectively bred for high locomotor activity, Behav. Genet., 2011, vol. 41, pp. 615–624.PubMedCrossRefGoogle Scholar
  7. Harley, V.R., Clarkson, M.J., and Argentaro, A., The molecular action and regulation of the testis-determining factors SRY (sex-determining region on the Y chromosome) and SOX9 [SRY-related high-mobility group (HMG) box 9], Endocr. Rev., 2003, vol. 24, pp. 466–487.PubMedCrossRefGoogle Scholar
  8. Kashimada, K. and Koopman, P., Sry: the master switch in mammalian sex determination, Development, 2010, vol. 137, pp. 3921–3930.PubMedCrossRefGoogle Scholar
  9. Kishikawa, H., Tateno, H., and Yanagimachi, R., Chromosome analysis of BALB/c mouse spermatozoa with normal and abnormal head morphology, Biol. Reprod., 1999, vol. 61, pp. 809–812.PubMedCrossRefGoogle Scholar
  10. Krzanowska, H., Styrna, J., and Wabik-Sliz, B., Analysis of sperm quality in recombinant inbred mouse strains: correlation of sperm head shape with sperm abnormalities and with the incidence of supplementary spermatozoa in the perivitelline space, J. Reprod. Fertil., 1995, vol. 104, pp. 347–354.PubMedCrossRefGoogle Scholar
  11. Navarro-Costa, P. and Plancha, C.E., The AZFc region of the Y chromosome: at the crossroads between genetic diversity and male infertility, Hum. Reprod. Update, 2010, vol. 16, pp. 525–542.PubMedCrossRefGoogle Scholar
  12. Osadchuk, A.V. and Naumenko, E.V., Genetic-endocrine and ethological mechanisms of differential reproduction. I. Comparative genetic analysis of the basal level of testosterone in the blood plasma, the relative weight of the testes and accessory gonads in male laboratory mice, Genetika, 1983, vol. 19, no. 8, pp. 1265–1273.PubMedGoogle Scholar
  13. Osadchuk, L.V., Tupikin, A.E., Morozov, I.V., et al., Phenotypic variation of spermatogenesis and a search for associations with genetic polymorphism in 13 inbred mouse strains, Russ. J. Genet., 2012, vol. 48, no. 8, pp. 823–830.CrossRefGoogle Scholar
  14. Styrna, J., Kilarski, W., and Krzanowska, H., Influence of the CBA genetic background on spermmorphology and fertilization efficiency in mice with a partial Y chromosome deletion, Reproduction, 2003, vol. 126, pp. 579–588.PubMedCrossRefGoogle Scholar
  15. Verhoeven, G., Willems, A., Denolet, E., and Swinnen, V., Androgens and spermatogenesis: lessons from transgenic mouse models, Phil. Trans. R. Soc., 2010, vol. 365, pp. 1537–1556.CrossRefGoogle Scholar
  16. Zheng, F. and Yang, P.J., Regulation of male fertility by X-linked genes, J. Androl., 2010, vol. 31, pp. 79–84.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2013

Authors and Affiliations

  • M. A. Kleshchev
    • 1
  • A. V. Osadchuk
    • 1
  • L. V. Osadchuk
    • 1
  1. 1.Institute of Cytology and GeneticsRussian Academy of Sciences, Siberian BranchNovosibirskRussia

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