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Age-Related Changes in the Metabolomic Composition of Macaque (Macaca fascicularis) Ocular Tissues

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Abstract

The study is aimed at determining age-related changes in ocular tissues of crab-eating macaque (Macaca fascicularis). To this end, we measured the concentrations of a total of 71 major metabolites in aqueous humor, vitreous humor, and lens of two groups of animals, young (4-year-old, n = 6) and aged (21-year-old and 27-year-old, n = 2) macaques. Significant age-related changes were revealed for all three tissues. In the lens, the most significant changes were found for cytoprotective compounds – antioxidants, osmolytes, and molecular ultraviolet (UV) filters: the concentrations of these metabolites in the lenses of aged animals are much lower. The observed changes contribute to increased oxidative stress and predispose the lens to the development of cataracts. The majority of cytoprotective metabolites are synthetized in the lens epithelium. Findings of this work indicate that the observed age-related changes may be caused by the impairment of the lens epithelial cells leading to the increase of oxidative stress in the lens nucleus and developing of age-related cataracts.

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ACKNOWLEDGMENTS

We thank the Ministry of Science and Higher Education of the RF for access to NMR equipment.

Funding

This research was funded by the Russian Science Foundation, grant no. 22-23-00021, https://rscf.ru/project/22-23-00021/.

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Authors and Affiliations

  1. Laboratory of Proteomics and Metabolomics, International Tomography Center Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    V. V. Yanshole, M. V. Fomenko, L. V. Yanshole, N. A. Osik & Y. P. Tsentalovich

  2. Department of Physics, Novosibirsk State University, 630090, Novosibirsk, Russia

    V. V. Yanshole, M. V. Fomenko & N. A. Osik

  3. National Research Centre “Kurchatov Institute,” Research Institute of Medical Primatology, 354376, Sochi, Russia

    E. Y. Radomskaya & D. V. Bulgin

Authors
  1. V. V. Yanshole
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  2. M. V. Fomenko
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  3. L. V. Yanshole
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  4. N. A. Osik
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  5. E. Y. Radomskaya
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  6. D. V. Bulgin
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  7. Y. P. Tsentalovich
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Corresponding author

Correspondence to Y. P. Tsentalovich.

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ETHICS APPROVAL AND CONSENT TO PARTICIPATE

The research was carried out in line with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research and the European Union Directive 2010/63/EU on the protection of animals used for scientific purposes, and approved by bioethics committee of the Research Institute of Medical Primatology (Record 80/1 from December 2, 2021).

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The authors of this work declare that they have no conflicts of interest.

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Supplementary Information

13329_2024_1284_MOESM1_ESM.xlsx

Supplementary materials are available for this article. A table of metabolite concentrations in AH, VH, and lens of individual specimens of M. fascicularis is available in the form of Excel file.

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Yanshole, V.V., Fomenko, M.V., Yanshole, L.V. et al. Age-Related Changes in the Metabolomic Composition of Macaque (Macaca fascicularis) Ocular Tissues. Adv Gerontol 13, 9–15 (2023). https://doi.org/10.1134/S2079057023600246

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  • DOI: https://doi.org/10.1134/S2079057023600246

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