The goal of this study was to examine the content of aryl-hydrocarbon receptor nuclear translocator (ARNT) in fibroblasts of human dermis from 20 weeks of pregnancy to the age of 85 years and to define the role of ARNT in age-dependent changes in the fibroblast number in the dermis. ARNT and proliferating cell nuclear antigen (PCNA) were detected with an indirect immunohistochemical technique. The results showed that the proportion of fibroblasts with positive staining for ARNT in the dermis gradually decreased from 20 weeks of pregnancy to the age of 40 years. There is a sharp increase in the percentage of ARNT-positive fibroblasts in the dermis after the age of 41 years; it reaches the maximum in the 60- to 85-year-old age group. The total number and percentage of PCNA-positive fibroblasts in the dermis decreased with age, most significantly from the antenatal period until 40 years of life. Age-related changes in the ARNT content in fibroblasts are not statistically associated with an age-related decrease in total number or percentage of PCNA-positive fibroblasts in the dermis.
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The study was supported by the Russian Foundation for Basic Research and government of the Chuvash Republic (19-415-210 001).
Conflicts of interest. The authors declare that they have no conflict of interest.
Statement of compliance with standards of research involving humans as subjects. The study was approved by the ethical committee of the Medical Faculty of the Ulyanov Chuvash State University.
Translated by L. Solovyova
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Gunin, A.G., Golubtzova, N.N. & Kornilova, N.K. Aryl-Hydrocarbon Receptor Nuclear Translocator (ARNT) in Human Skin with Aging. Adv Gerontol 10, 388–392 (2020). https://doi.org/10.1134/S2079057020040086