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The Influence of Metformin on Age-Related Changes in the Number and Proliferation of Dermal Fibroblasts in Mice

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Abstract

The goal of the work was to examine the effects of metformin on age-related changes in the number and proliferation of dermal fibroblasts in mice. The dermis was examined in 5-month-old mice that received drinking water with metformin at a concentration of 500 mg/L from the age of 2 months (within 90 days). Five-month-old mice that received drinking water without metformin were used as a control. Material from 2-month-old mice was also used in the work. We have counted the total number of dermal fibroblasts and a portion of fibroblasts with positive staining for the Ki-67 antigen. The results showed that there was a decrease in the total number of mouse dermal fibroblasts by 42.3% from two up to 5 months of life and in the proportion of fibroblasts with positive staining for Ki-67 by 12%. The total number of dermal fibroblasts in 5-month-old mice was 13.4% lower under the effect of metformin. The proportion of fibroblasts with positive staining for Ki-67 was reduced by 27.3% as compared to animals that did not receive metformin. Thus, the age-related decrease in dermal fibroblasts of mice is due to the decline in their proliferation activity. Metformin has an inhibitory effect on the proliferation of dermal fibroblasts in mice.

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ACKNOWLEDGMENTS

This study was supported by the Russian Foundation for Basic Research, projects nos. 16-44-210018 and 18-04-00024.

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Authors and Affiliations

  1. Chuvash State University, 428015, Cheboksary, Russia

    A. G. Gunin, N. N. Golubtsova, N. O. Subbotkina & A. S. Subbotkin

Authors
  1. A. G. Gunin
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  2. N. N. Golubtsova
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  3. N. O. Subbotkina
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  4. A. S. Subbotkin
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Correspondence to A. G. Gunin.

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Conflict of interests. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The study was approved by the Ethics Committee at the Faculty of Medicine (Ulyanov Chuvash State University).

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Translated by E. Makeeva

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Gunin, A.G., Golubtsova, N.N., Subbotkina, N.O. et al. The Influence of Metformin on Age-Related Changes in the Number and Proliferation of Dermal Fibroblasts in Mice. Adv Gerontol 9, 81–85 (2019). https://doi.org/10.1134/S2079057019010077

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  • DOI: https://doi.org/10.1134/S2079057019010077

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