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The effect of water deprivation and α-tocopherol acetate on the expression of apoptosis marker proteins

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Abstract

Ontogenetic differences in the rate of apoptosis and expression of apoptosis signal proteins, as well as in the effects of the antioxidant α-tocopherol acetate on the neurons of magnocellular hypothalamic centers in BALB-c mice under stress were revealed. Hypohydration stress was found to initiate neuron apoptosis in both young and old animals. The stress-protective effects of α-tocopherol acetate were more pronounced in young animals. The following tendencies were established in the course of immunohistochemical studies of pro- and antiapoptotic protein expression. Dehydration results in increased synthesis of the proapoptotic protein Bax in neurosecretory cells of the hypothalamus in young mice and decreases the antiapoptotic effect of the proteins of the Bcl-2 family independently of the animal’s age, thus promoting neuron death. Apparently, the more pronounced antiapoptotic effect of α-tocopherol acetate in young mice under stress, if compared to elderly animals, is due to the rapid induction of compensatory mechanisms, namely, a decrease in the synthesis of the proapoptotic proteins p53 and Bax and increase in the expression of the antiapoptotic protein Bcl-2.

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References

  1. Gubareva, E.A., Nikitina, L.S., Dorofeeva, N.A., et al., Influence of Bcl-2 on ERK Activity of MAPK-Casperase Module in SOYa Neurons of Rat Hypotalamus, in Neirokhimicheskie mekhanizmy formirovaniya adaprivnykh i patologicheskikh sistoyanii mozga: Konf. s mezhd. uch. SPb-Koltushi, 10–12 sent. 2008 (Neurochemical Mechanisms of Adaptive and Pathological Brain Status Formation, Conference with International Participation, St. Petersburg-Koltushi, September 10–12, 2008), 2008, pp. 36–37.

  2. Nikitina, L.S. ERK1/2 Module of MARK Signal Cascade Mediates the Influence of Signal Proteins of Apoptosis Bcl-2 and p53 on Secretory Activity of Vasopressin Neurons of Supraoptical Nucleus of Hypotalamus, in Gormonal’nye mekhanismy adaptatsii: Vseros. simp. s mezhdunar. uch., SPb (Hormonal Mechanisms of Adaptation, All-Russian Symposium with International Participation, St. Petersburg) 2007, p. 58.

  3. Novikov, V.S., Bulavin, D.V., and Tsygan, V.N., Molecular Mechanisms of Cell Apoptosis Initiation, in Programirovannaya kletochnaya gibel’ (Programmed Cell Death), St. Petersburg: Nauka, 1996, pp. 30–51.

    Google Scholar 

  4. Chernigovskaya, E.V., Nikitina, L.S., Dorofeeva, N.A., et al., Participation of Signal Proteins of Apoptosis in Regulation of Functional Activity of Neurons of the Rat Brain, in Nauchnye trudy I S”ezda fiziologov SNG (Scientific Transactions of the I Congress of Physiologists of CIS), Dagomys, 2005, vol. 2, p. 53.

    Google Scholar 

  5. Chernigovskaya, E.V., Nikitina, L.S., and Glazova, M.V., Intracellular Regulatory Mechanisms of Vasopressin Biosynthesis, in Tezisy dokladov VIII Vserossiiskoi konferentsii “Neiroendokrinologiya-2010”, SPb, 20–22 aprelya 2010 (Neuroendocrinology-2010, Abstract of Papers of the VIII All-Russian Conference, St. Petersburg, April 20–22, 2010), 2010, pp. 146–147.

  6. Adams, J.M. and Cory, S., The Bcl-2 Protein Family: Arbiters of Cell Survival, Science, 1998, vol. 281, pp. 1322–1326.

    Article  PubMed  CAS  Google Scholar 

  7. Boise, L.H., Gonzales-Garcia, M., Rostema, C.E., et al., Bcl-x, Bcl-2 Related Gene that Function as a Dominant Regulator of Apoptotic Cell Death, Cell, 1993, vol. 74, pp. 597–608.

    Article  PubMed  CAS  Google Scholar 

  8. Dietrich, J.B., Apoptosis and Anti-Apoptosis Genes in the Bcl-2 Family, Arch. Physiol. Biochem., 1997, vol. 105(2), pp. 125–135.

    Article  PubMed  CAS  Google Scholar 

  9. Fyhr, I.M., Lindberg, C., and Oldfors, A., Expression of Bcl-2 in Inclusion Body Myositis, Acta Neurol. Scand., 2002, vol. 105(5), pp. 403–407.

    Article  PubMed  Google Scholar 

  10. Jacobson, M.D., Burne, J.F., King, M.P., et al., Bcl-2 Blocks Apoptosis in Cells Lacking Mitochondrial DNA, Nature, 1993, vol. 361(6410), pp. 365–366.

    Article  Google Scholar 

  11. Hockenbery, D., Oltvai, Z.N., Milliman, C.L., and Kotmeyer, S.J., Bcl-2 Functions in an Antioxidant Pathway to Prevent Apoptosis, Cell, 1993, vol. 75, pp. 241–251.

    Article  PubMed  CAS  Google Scholar 

  12. Howard, S., Bottino, C., Brooke, S., et al., Neuroprotective Effects of Bcl-2 Overexpression in Hippocampal Cultures: Interactions with Pathways of Oxidative Damage, J. Neurochem., 2002, vol. 83(4), pp. 914–923.

    Article  PubMed  CAS  Google Scholar 

  13. Kane, D.C., Sarafian, T.A., Antou, R., et al., Bcl-2 Inhibition of Neural Death Decreased Generation of Reaction Oxygen Species, Science, 1993, vol. 262, pp. 1274–1277.

    Article  PubMed  CAS  Google Scholar 

  14. Kaushal, V., Kaushal, G., and Mehta, P., Differential Toxicity of Anthracyclines on Cultured Endothelial Cells, Endothelium, 2004, vol. 11(5–6), pp. 253–258.

    Article  PubMed  CAS  Google Scholar 

  15. Kirkland, R.A., Windelborn, J.A., Kasprzak, J.M., Franklin, J.L., A Bax-induced Pro-Oxidant State is Critical for Cytochrome c Release during Programmed Neuronal Death, J. Neurosci., 2002, vol. 22(15), pp. 6480–6490.

    PubMed  CAS  Google Scholar 

  16. Kroemer, G., Mitochondrial Implication in Apoptosis, Cell Death Different., 1997, vol. 4, pp. 443–456.

    Article  CAS  Google Scholar 

  17. Kumar, S., The Bcl-2 Family of Proteins and Activation on the ICE/CED-3 Family of Proteases: A Balancing Act in Apoptosis? Cell Death Different., 1997, vol. 4, pp. 2–3.

    Article  CAS  Google Scholar 

  18. Michels, J., Johnson, P. W., and Packham, G., Mcl-1, Int. J. Biochem. Cell Biol., 2005, vol. 37(2), pp. 267–271.

    Article  PubMed  CAS  Google Scholar 

  19. Opferman, J.T., Iwasaki, H., Ong, C.C., et al., Obligate Role of Anti-Apoptotic Mcl-1 in the Survival of Hematopoietic Stem Cells, Science, 2005, vol. 307(5712), pp. 1101–1104.

    Article  Google Scholar 

  20. Rosse, T., Olivier, R., Monney, L., et al., Bcl-2 Prolongs Cell Survival after Bax-Induced Release of Cytochrome c, Nature, 1998, vol. 391, pp. 496–499.

    Article  PubMed  CAS  Google Scholar 

  21. Steinman, H.M., The Bcl-2 Oncoprotein Functions as a Prooxidant, J. Biol. Chem., 1995, vol. 270(8), pp. 3487–3490.

    PubMed  CAS  Google Scholar 

  22. Tao, W., Kurschner, C., Morgan, J.I., Modulation of Cell Death in Yeast by the Bcl-2 Family of Proteins, J. Biol. Chem., 1997, vol. 272(24), pp. 15547–15552.

    Article  PubMed  CAS  Google Scholar 

  23. Tobiume, K., Involvement of Bcl-2 Family Proteins in p53-Induced Apoptosis, J. Nippon. Med. Sch., 2005, vol. 72(4), pp. 192–193.

    Article  Google Scholar 

  24. Weng, C., Li, Y., Xu, D., et al., Specific Cleavage of Mcl-1 by Caspase-3 in Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-Induced in Jurkat Leukemia T-Cells, J. Biol. Chem., 2005, vol. 280(11), pp. 10491–10500.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Astrakhan State University, ul. Tatishcheva 20a, Astrakhan’, 414056, Russia

    Yu. V. Azizova, D. L. Teplyi & O. N. Pozdnyakova

  2. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. M. Toreza 44, St. Petersburg, 194223, Russia

    E. D. Bazhanova

Authors
  1. Yu. V. Azizova
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  2. D. L. Teplyi
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  3. E. D. Bazhanova
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  4. O. N. Pozdnyakova
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Correspondence to Yu. V. Azizova.

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Original Russian Text © Yu.V. Azizova, D.L. Teplyi, E.D. Bazhanova, O.N. Pozdnyakova, 2011, published in Uspekhi Gerontologii, 2011, Vol. 24, No. 2, pp. 220–224.

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Azizova, Y.V., Teplyi, D.L., Bazhanova, E.D. et al. The effect of water deprivation and α-tocopherol acetate on the expression of apoptosis marker proteins. Adv Gerontol 2, 38–42 (2012). https://doi.org/10.1134/S2079057012010031

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