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Is it possible “to cancel” the aging process of cell cultures under optimal cultivation conditions?

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Abstract

The characteristics of epigenotypes of Dunaliella viridis Teod. cells have been investigated during chronological and replicative aging. It was shown that by the 40th day of cumulative cultivation, which coincided with the stationary phase of growth, the DNA content doubled, the TG content tripled, the content of β-carotin doubled, carbonilated proteins doubled, and the content of RNA decreased in the cells of Dunaliella viridis compared to the cells in the exponential phase of growth; i.e., by the 40th day of cultivation, age-dependent epigenotype develops. Four subcultures were obtained which were transplanted for two years in a midlogarithmic growth phase (subculture-10), early stationary phase (subculture-20), a midstationary growth phase (subculture-30), and a late stationary phase (subculture-40). The epigenotype of subculture-10 was shown to remain unchanged for two years of cultivation, i.e., there was no replicative aging. At the same time, despite the fact that subculture-20 maintained the epigenotype characteristic of a young culture for quite a long time (no less than 40 passages), it displayed age-dependent changes. Marked age-dependent changes in the epigenotype during the cultivation were revealed in subculture-30; subculture-40 was characterized by an unstable epigenotype. The conditions for cultivation were shown to define the intensity of replicative aging in Dunaliella viridis.

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Authors and Affiliations

  1. Research Institute of Biology, Vasyl Karazin Kharkov National University, pl. Svobodi 4, Kharkov, 61077, Ukraine

    A. I. Bozhkov, M. K. Kovaleva & N. G. Menzyanova

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  1. A. I. Bozhkov
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  2. M. K. Kovaleva
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  3. N. G. Menzyanova
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Correspondence to A. I. Bozhkov.

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Original Russian Text © A.I. Bozhkov, M.K. Kovaleva, N.G. Menzyanova, 2011, published in Uspekhi Gerontologii, 2011, Vol. 24, No. 1, pp. 26–37.

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Bozhkov, A.I., Kovaleva, M.K. & Menzyanova, N.G. Is it possible “to cancel” the aging process of cell cultures under optimal cultivation conditions?. Adv Gerontol 1, 284–294 (2011). https://doi.org/10.1134/S2079057011040047

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  • DOI: https://doi.org/10.1134/S2079057011040047

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