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Creating a Tissue-Specific Microdispersed Matrix from a Decellularized Porcine Liver

  • NEW METHODS OF TREATMENT AND PRODUCTION OF MATERIALS WITH REQUIRED PROPERTIES
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Inorganic Materials: Applied Research Aims and scope

Abstract

The main problem with decellularization of liver tissue as a tissue-specific matrix/scaffold in liver bioengineered structures is the need to maximize the preservation of the original three-dimensional structure of the tissue and the main components of its extracellular matrix (ECM) while removing cells and genetic material. The attempts to use the existing protocols for the decellularization of other tissues and organs have been unsuccessful. The aim of the work is to develop a method for creation of a tissue-specific microdispersed matrix from decellularized porcine liver (TMM DLp). The protocol for decellularization of porcine liver (Lp) fragments has been developed on the basis of the complex application of chemical (sodium dodecyl sulfate and Triton X100), biochemical (DNase I), and physical (supercritical CO2) methods for treatment of the initial tissue. As a result of the found optimal conditions for decellularization of Lp with subsequent cryomicronization of decellularized DLp, an injectable form of the microdispersed tissue-specific matrix was obtained, which represents decellularized porcine liver microparticles with the size of 100–200 μm with the residual amount of DNA no more than 10 ± 1.5 ng/mg (less than 1.0%), with the preservation of the microstructure and basic composition of the liver ECM. According to the in vitro assessment, biocompatible properties of tissue-specific matrix samples meet the criteria of biological safety for cytotoxicity and hemolytic activity.

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REFERENCES

  1. Sevastianov, V.I., Technologies of tissue engineering and regenerative medicine, Vestn. Transplant. Iskusst. Organ., 2014, vol. 16, no. 3, pp. 93–108. https://doi.org/10.15825/1995-1191-2014-3-93-108

    Article  Google Scholar 

  2. Pina, S., Ribeiro, V.P., Marques, C.F., Maia, F.R., Silva, T.H., Reis, R.L., and Oliveira, J.M., Scaffolding strategies for tissue engineering and regenerative medicine applications, Materials, 2019, vol. 12, no. 11, art. ID 1824. https://doi.org/10.3390/ma12111824

    Article  CAS  PubMed Central  Google Scholar 

  3. Rossi, E.A., Quintanilha, L.F., Nonaka, C.K.V., and Souza, B.S.F., Advances in hepatic tissue bioengineering with decellularized liver bioscaffold, Stem Cells Int., 2019, vol. 2019, art. ID 2693189. https://doi.org/10.1155/2019/2693189

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Crapo, P.M., Gilbert, T.W., and Badylak, S.F., An overview of tissue and whole organ decellularization processes, Biomaterials, 2011, vol. 32, no. 12, pp. 3233–3243.

    Article  CAS  Google Scholar 

  5. Tajima, K., Yagi, H., and Kitagawa, Y., Human-scale liver harvest and decellularization for preclinical research, in Decellularized Scaffolds and Organogenesis, Turksen, K., Ed., New York: Humana, 2018, vol. 1577, pp. 327–335.

    Google Scholar 

  6. Hassanein, W., Uluer, M.C., Langford, J., Woodall, J.D., Cimeno, A., Dhru, U., Werdesheim, A., Harrison, J., Rivera-Pratt, C., Klepfer, S., Khalifeh, A., Buckingham, B., Brazio, P.S., Parsell, D., Klassen, C., et al., Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold, Organogenesis, 2017, vol. 13, no. 1, pp. 16–27.

    Article  CAS  Google Scholar 

  7. Wang, Y., Bao, J., Wu, Q., Zhou, Y., Li, Y., Wu, X., Shi, Y., Li, L., and Bu, H., Method for perfusion decellularization of porcine whole liver and kidney for use as a scaffold for clinical-scale bioengineering engrafts, Xenotransplantation, 2015, vol. 22, no. 1, pp. 48–61.

    Article  CAS  Google Scholar 

  8. Ozlu, B., Ergin, M., Budak, S., Tunali, S., Yildirim, N., and Erisken, C., A bioartificial rat heart tissue: Perfusion decellularization and characterization, Int. J. Artif. Organs, 2019, vol. 42, no. 12, pp. 757–764.

    Article  CAS  Google Scholar 

  9. Bölükbas, D.A., De Santis, M.M., Alsafadi, H.N., Doryab, A., and Wagner, D.E., The preparation of decellularized mouse lung matrix scaffolds for analysis of lung regenerative cell potential, in Mouse Cell Culture, Bertoncello, I., Ed., New York: Humana, 2019, vol. 1940, pp. 275–295.

    Google Scholar 

  10. Obata, T., Tsuchiya, T., Akita, S., Kawahara, T., Matsumoto, K., Miyazaki, T., Masumoto, H., Kobayashi, E., Niklason, L.E., and Nagayasu, T., Utilization of natural detergent potassium laurate for decellularization in lung bioengineering, Tissue Eng., Part C, 2019, vol. 25, no. 8, pp. 459–471.

    Article  CAS  Google Scholar 

  11. Kuna, V.K., Kvarnström, N., Elebring, E., and Holgersson, S.S., Isolation and decellularization of a whole porcine pancreas, J. Visualized Exp., 2018, no. 140, art. ID 58302. https://doi.org/10.3791/58302

  12. Nemec, E.A., Kirsanova, L.A., Basok, J.B., Shagidulin, M.J., Volkova, E.A., Metelsky, S.T., and Sevastianov, V.I., Technology features of decellularization of human liver fragments as tissue-specific fine-grained matrix for cell-engineering liver construction, Vestn. Transplant. Iskusst. Organ., 2017, vol. 19, no. 4, pp. 70–77.

    Google Scholar 

  13. Antons, J., Marascio, M.G., Aeberhard, P., Weissenberger, G., Hirt-Burri, N., Applegate, L.A., Bourban, P.E., and Pioletti, D.P., Decellularised tissues obtained by a CO2-philic detergent and supercritical CO2, Eur. Cell Mater., 2018, vol. 36, pp. 81–95. https://doi.org/10.22203/eCM.v036a15

    Article  CAS  PubMed  Google Scholar 

  14. Bernhardt, A., Wehrl, M., Paul, B., Hochmuth, T., Schumacher, M., Schütz, K., and Gelinsky, M., Improved sterilization of sensitive biomaterials with supercritical carbon dioxide at low temperature, PLoS One, 2015, vol. 10, no. 6, art. ID e0129205. https://doi.org/10.1371/journal.pone.0129205

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. GOST (State Standard) ISO 10993-5-2011: Medical Devices. Biological Evaluation of Medical Devices. Part 5. Tests for in vitro Cytotoxicity, 2014.

  16. GOST (State Standard) ISO 10993-4-2011: Medical Devices. Biological Evaluation of Medical Devices. Part 4. Selection of Tests for Interactions with Blood, 2013.

  17. Zhang, L., Guan, Z., Ye, J.-S., Yin, Y.-F., Stoltz, J.-F., and de Isla, N., Research progress in liver tissue engineering, Bio-Med. Mater. Eng., 2017, vol. 28, no. s1, pp. S113–S119. https://doi.org/10.3233/BME-171632

    Article  Google Scholar 

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ACKNOWLEDGMENTS

This study was carried out with partial financial support from the Russian Foundation for Basic Research, grant no. 18-29-06012.

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Authors and Affiliations

  1. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182, Moscow, Russia

    A. D. Kirillova, Yu. B. Basok, A. M. Grigoryev, L. A. Kirsanova, E. A. Nemets & V. I. Sevastianov

  2. Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russia

    A. E. Lazhko

Authors
  1. A. D. Kirillova
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  2. Yu. B. Basok
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  3. A. E. Lazhko
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  4. A. M. Grigoryev
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  5. L. A. Kirsanova
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  6. E. A. Nemets
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  7. V. I. Sevastianov
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Corresponding authors

Correspondence to A. D. Kirillova, Yu. B. Basok, A. E. Lazhko, A. M. Grigoryev, L. A. Kirsanova, E. A. Nemets or V. I. Sevastianov.

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Translated by L. Solovyova

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Kirillova, A.D., Basok, Y.B., Lazhko, A.E. et al. Creating a Tissue-Specific Microdispersed Matrix from a Decellularized Porcine Liver. Inorg. Mater. Appl. Res. 12, 812–819 (2021). https://doi.org/10.1134/S2075113321030199

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  • DOI: https://doi.org/10.1134/S2075113321030199

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