Materials for 3D printing of porous composite materials (CM) that are based on sodium alginate–tricalcium phosphate are developed. Physicochemical and biological studies of CM in vitro are performed using a model of two adherent cells lines, immortalized human fibroblasts (HF, strain 1608h TERT) and human osteosarcoma (MG-63) that are cultivated up to 21 days. The cytocompatibility and matrix properties are studied by an MTT assay.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Rodriguez, G.M., Naves, D.M., and Cannata, A.J.B., Bone metabolism, vascular calcifications and mortality: Associations beyond mere coincidence, J. Nephrol., 2005, vol. 18, pp. 458–463.
Fu, K., Xu, Q., Czernuszka, J., Triffitt, J.T., and Xia, Z., Characterization of a biodegradable coralline hydroxyapatite/ calcium carbonate composite and its clinical implementation, Biomed. Mater, 2013, vol. 8, p. 065007.
Venkatesan, J. and Kim, Se-K., Marine Biomaterials. Springer Handbook of Marine Biotechnology, Berlin: Springer-Verlag, 2015.
Gelinsky, M., Mineralised collagen as biomaterial and matrix for bone tissue engineering, in Fundamentals of Tissue Engineering and Regenerative Medicine Berlin: Springer-Verlag, 2009, pp. 485–493.
Serino, G., Rao, W., Iezzi, G., and Piattelli, A., Polylactide and polyglycolide sponge used in human extraction sockets: Bone formation following 3 months after its application, Clin. Oral. Implants Res., 2008, vol. 19, no. 1, pp. 26–31.
Samavedi, S., Whittington, A.R., and Goldstein, A.S., Calcium phosphate ceramics in bone tissue engineering: A review of properties and their influence on cell behavior, Acta Biomater., 2013, vol. 9, pp. 8037–8045.
Hench, L.L., Bioceramics: From concept to clinic, J. Am. Ceram., 1991, vol. 74, pp. 1487–1510.
Sarkisov, P.D., Mikhailenko, N.Yu., and Khavala, V.M., Biologicheskaya aktivnost’ materialov na osnove stekla i sistallov, Steklo Keram., 1993, vol. 9, pp. 10–16.
Karpova, S.G., Popov, A.A., Khvatov, A.V., Klenina, N.S., and Iordanskii, A.L., Effect of external influences on the structural and dynamic parameters of medicinal copolymers, Russ. J. Phys. Chem. B, 2011, vol. 5, pp. 148–155.
Suzuki, O., Octacalcium phosphate (OCP) - based bone substitute materials, Jap. Dental Sci. Rev., 2013, vol. 49, pp. 58–71.
Sun, J. and Tan, H., Alginate-based biomaterials for regenerative medicine applications, Materials, 2013, vol. 6, pp. 1285–1309.
Kundu, J., Pati, F., Shim, J.-H., and Cho, D.-W., Rapid Prototyping Technology for Bone Regeneration. Principles and Applications Cambridge: Woodhead, 2014.
Komlev, V.S., Barinov, S.M., and Koplik, E.V., A method to fabricate porous spherical hydroxyapatite granules intended for time-controlled drug release, Biomaterials, 2002, vol. 23, pp. 3449–3454.
Mossman, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays, J. Immunol. Methods, 1983, vol. 65, pp. 55–63.
Original Russian Text © V.S. Komlev, N.S. Sergeeva, A.Yu. Fedotov, I.K. Sviridova, V.A. Kirsanova, S.A. Akhmedova, A.Yu. Teterina, Yu.V. Zobkov, E.A. Kuvshinova, Ya.D. Shanskiy, S.M. Barinov, 2016, published in Materialovedenie, 2016, No. 3, pp. 38–42.
About this article
Cite this article
Komlev, V.S., Sergeeva, N.S., Fedotov, A.Y. et al. Investigation of physicochemical and biological properties of composite matrices in a alginate–calcium phosphate system intended for use in prototyping technologies during replacement of bone defects. Inorg. Mater. Appl. Res. 7, 630–634 (2016). https://doi.org/10.1134/S2075113316040158