Abstract
Enzymatic hydrolysis of chitosan (CTS) is studied by viscosity measurements, velocity sedimentation, and diffusion. It is found that the incubation of CTS in acetic acid solution is always accompanied by a decrease in the relative and intrinsic viscosity of CTS, but the chains are not destroyed in the process, as evidenced by the coincidence of the sedimentation constant and diffusion coefficient values with the values obtained for the initial CTS samples. The addition of enzymatic agents to the CTS solution results in a significant decrease in the sedimentation constant and an increase in the diffusion coefficient, indicating a decrease in molecular weight and the proceeding of enzymatic hydrolysis. Thus, the pattern of changes in the solution viscosity indexes, which can be affected by supramolecular structures of the polymer solution, has to be taken into account when using viscosity measurements.
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Khitin i khitozan: poluchenie, svoistva i primenenie (Chitin and Chitosan: Production, Properties and Application) Skryabin, K.G., Vikhoreva, G.A., and Varlamov, V.P., Eds., Moscow: Nauka, 2002.
Il’ina, A.V., Tkacheva, Yu.V., and Varlamov, V.P., Depolymerization of high-molecular-weight chitosan by the enzyme preparation celloviridine G20X, Priklad. Biokhim. Mikrobiol., 2002, vol. 38, pp. 132–135.
Mullagaliev, I.R., Aktuganov, G.E., and Melent’ev, A.I., Study of chitosan depolymerization by chitinolitic ferments of aerobic spores-formation bacteria, Materialy 8 mezhdunarodnoi konferentsii “Sovremennye perspektivy v issledovanii khitina i khitozana” (Proc. 8th Int. Conf. “Contemporary Perspectives in Study of Chitin and Chitosan), Kazan, 2006, pp. 305–309.
Vikhoreva, G.A., Rogovina, S.Z., Pchelko, O.M, and Gal’braikh, L.S., The phase state and rheological properties of chitosan-acetic acid-water system, Polymer Sci., Ser. B, 2001, vol. 43, pp. 166–170.
Fedoseeva, E.N., Semchikov, Yu.D., and Smirnova, L.A., Degradation of chitosan in solutions in the presence of a redox system, Polymer Sci., Ser. B, 2006, vol. 48, pp. 295–299.
Budtov, V.P., Fizicheskaya khimiya rastvorov polimerov (Physical Chemistry of Polymer Solutions), St. Petersburg: Khimiya, 1992.
Kulish, E.I., Chernova, V.V., Vil’danova, R.F., Volodina, V.P., and Kolesov, S.V., On the reason of chitosan fermentative hydrolysis under the action of some nonspecific ferments, Vestnik Bashkir. Univ., 2011, vol. 16, pp. 681–683.
Rafikov, S.R., Budtov, V.P., and Monakov, Yu.B., Vvedenie v fiziko-khimiyu rastvorov polimerov (Introduction in Physical Chemistry of Polymer Solutions), Moscow: Khimiya, 1978.
Coupland, J.N., Brathwaite, D., Fairley, P., and McClements, D.J., Effect of ethanol on the solubilization of hydrocarbon emulsion droplets in nonionic surfactant micelles, J. Colloid Interface Sci., 1997, vol. 190, no. 1, pp. 71–75.
Chernova, V.V., Kulish, E.I., Volodina, V.P., and Kolesov, S.V., Effect of non-specific ferments on chitosan, Materialy Devyatoi Mezhdunarodnoi konferentsii “Sovrem. Perspect. v Izuch. Khitin Khitozan” (Proc. 9th Int. Conf. “Contemporary Perspectives in the Study of Chitin and Chitosan”), Stavropol’, 2008, pp. 234–236.
Kulish, E.I., Chernova, V.V., Volodina, V.P., Torlopov, M.A., and Kolesov, S.V., Degradation of polysaccharides under the action of some non-specific ferments, Materialy Desyatoi Mezhdunarodnoi konferentsii “Sovrem. Perspect. v Izuch. Khitin Khitozan” (Proc. 10th Int. Conf. “Contemporary Perspectives in the Study of Chitin and Chitosan”), Nizhni Novgorod, 2010, pp. 274–278.
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Original Russian Text © E.I. Kulish, V.V. Chernova, V.P. Volodina, S.V. Kolesov, 2013, published in Perspektivnye Materialy, 2013, No. 10, pp. 29–33.
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Kulish, E.I., Chernova, V.V., Volodina, V.P. et al. Peculiarities of viscometric studies of enzymatic hydrolysis of chitosan. Inorg. Mater. Appl. Res. 5, 164–167 (2014). https://doi.org/10.1134/S2075113314020117
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DOI: https://doi.org/10.1134/S2075113314020117