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Features of adsorption of globular proteins by nanoporous silica

  • Molecular and Supramolecular Structures at the Interfaces
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Abstract

The adsorption of globular protein macromolecules, bovine serum albumin, and gamma globulin from aqueous solutions at 293 K by nanoporous silica sorbents is investigated. The dependences of the maximum value of adsorption of albumin and gamma globulin from aqueous solutions on the size of nanopores of silica sorbent are obtained. Based on this dependence, the sizes of macromolecular protein structures formed during the adsorption process are determined. A method for evaluating the thickness of adsorbed layers of protein macromolecules on silica surface is proposed. The behavior features of globular proteins in silica nanopores are discussed.

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References

  1. Dijt, J., Cohen, Stuart M., and Fleer, G., Macromolecules, 1994, vol. 27, p. 3207.

    Article  CAS  ADS  Google Scholar 

  2. Huinink, H. P., de Keizer, A., Leermakers, F. A. M., and Lyklema, J., Langmuir, 1997, vol. 13, p. 6452.

    Article  CAS  Google Scholar 

  3. Khokhlova, T. D., Vestn. Mosk. Un-Ta, Ser. 2, Khimiya, 2002, vol. 43, no. 3, p. 144.

    CAS  Google Scholar 

  4. El’tekova, N. A. and El’tekov, A. Yu., Zh. Fiz. Khim., 2000, vol. 74, p. 1075 [Russ. J. Phys. Chem. (Engl. Transl.), vol. 74].

    Google Scholar 

  5. Fang, F. and Szleifer, I., Langmuir, 2002, vol. 18, p. 5497.

    Article  CAS  Google Scholar 

  6. Kawasaki, K., Kambara, M., Matsumura, H., and Norde, W., Colloids Surf., 2003, vol. 32, p. 321.

    Article  CAS  Google Scholar 

  7. Vinu, A., Murugesan, V., and Hartmann, M., J. Phys. Chem. B, 2004, vol. 108, p. 7323.

    Article  CAS  Google Scholar 

  8. Salis, A., Meloni, D., Ligas, S., et al., Langmuir, 2005, vol. 21, p. 5511.

    Article  CAS  PubMed  Google Scholar 

  9. Bosker, W. T. E., Iakovlev, P. A., Norde, W., and Cohen, Stuart M., J. Colloid Interface Sci., 2005, vol. 286, p. 496.

    Article  CAS  PubMed  Google Scholar 

  10. Seitz, R., Brings, R., and Geiger, R., Appl. Surf. Sci., 2005, vol. 252, p. 154.

    Article  CAS  ADS  Google Scholar 

  11. Proteins at Solid-Liquid Interfaces Series: Principles and Practice, Dejardin P., Ed., Springer, 2006.

  12. Matsumura, H. and Saburi, M., Colloids Surf., 2006, vol. 47, p. 146.

    Article  CAS  Google Scholar 

  13. Van der Veen, M., Cohen, Stuart M., and Norde, W., Colloids Surf., 2007, vol. 54, p. 136.

    Article  Google Scholar 

  14. Hofs, B., Brzozowska, A., de Keizer, A., et al., J. Colloid Interface Sci., 2008, vol. 325, p. 309.

    Article  CAS  PubMed  Google Scholar 

  15. Tsapikouni, Th. S. and Missirlis, Y.F., Mat. Sci. Eng. B, 2008, vol. 152, p. 2.

    Article  CAS  Google Scholar 

  16. Reichelt, S. and Eichhorn, K. J., Aulich. D., et.al, Colloids Surf., 2009, vol. 69, p. 169.

    Article  CAS  Google Scholar 

  17. El’tekov, A. Yu. and El’tekova, N. A., Zh. Fiz. Khim., 2004, vol. 78, p. 1506 [Russ. J. Phys. Chem. (Engl. Transl.), vol. 78].

    Google Scholar 

  18. El’tekov, A. Yu., J. Chromatogr., A, 2005, vol. 1100, p. 15.

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Leninskii pr. 31, Moscow, 117071, Russia

    N. A. El’tekova & A. Yu. Eltekov

Authors
  1. N. A. El’tekova
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  2. A. Yu. Eltekov
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Correspondence to N. A. El’tekova.

Additional information

Original Russian Text © N.A. El’tekova, A.Yu. Eltekov, 2010, published in Fizikokhimiya Poverkhnosti i Zashchita Materialov, 2010, Vol. 46, No. 1, pp. 56–59.

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El’tekova, N.A., Eltekov, A.Y. Features of adsorption of globular proteins by nanoporous silica. Prot Met Phys Chem Surf 46, 60–63 (2010). https://doi.org/10.1134/S2070205110010089

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  • DOI: https://doi.org/10.1134/S2070205110010089

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