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Biocatalytic Conversion of Semi-Finished Hardwood into Sugars: Enzymatic Hydrolysis at High Concentrations of the Substrate

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Abstract

Exhaustive enzymatic hydrolysis is performed for semi-bleached sulfate hardwood cellulose (a semi-finished pulp and paper product) at ultra-high concentrations of it in a reaction mixture (up to 300 g/L per dry compound). Russian commercial enzyme preparations are used for hydrolysis. The best seems to be Agroxil Plus, which has high cellulase and endoxylanase activities. A total of 290 g/L of sugars (including 210 g/L of glucose and 30 g/L of xylose) is obtained using Agroxil Plus (20 mg protein/1 g substrate) in combination with an auxiliary β-glucosidase enzyme preparation (2 mg protein/1 g substrate) at an initial semi-bleached cellulose concentration of 300 g/L. The dosage of Agroxil Plus can be halved (10 mg of protein/1 g of substrate with a total concentration of semi-bleached cellulose of 300 g/L) with a high yield of hydrolysis product (270 g/L of sugars, including 200 g/L of glucose and 30 g/L of xylose), due to the fractional addition of a substrate.

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ACKNOWLEDGMENTS

This work was performed on scientific equipment at the Shared Facilities Center “Industrial Biotechnologies” of the Federal Research Center Fundamentals of Biotechnology of the Academy of Sciences.

Funding

This work was supported by the RF Ministry of Science and Higher Education.

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Authors and Affiliations

  1. Federal Research Center Fundamentals of Biotechnology, Russian Academy of Sciences, 119071, Moscow, Russia

    M. V. Semenova, A. M. Rozhkova, E. G. Kondratyeva, I. A. Shashkov, A. D. Satrutdinov & A. P. Sinitsyn

  2. Moscow State University, 119991, Moscow, Russia

    V. D. Telitsin, A. M. Rozhkova & A. P. Sinitsyn

  3. Tekhservis, 164900, Novodvinsk, Russia

    Ya. A. Gareeva & A. M. Kryazhev

  4. Arkhangelsk Pulp & Paper Mill, 164900, Arkhangelsk, Russia

    V. G. Moseev

Authors
  1. M. V. Semenova
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  2. V. D. Telitsin
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  3. A. M. Rozhkova
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  4. E. G. Kondratyeva
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  5. I. A. Shashkov
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  6. A. D. Satrutdinov
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  7. Ya. A. Gareeva
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  8. V. G. Moseev
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  10. A. P. Sinitsyn
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Corresponding author

Correspondence to M. V. Semenova.

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Translated by A. Tulyabaev

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Semenova, M.V., Telitsin, V.D., Rozhkova, A.M. et al. Biocatalytic Conversion of Semi-Finished Hardwood into Sugars: Enzymatic Hydrolysis at High Concentrations of the Substrate. Catal. Ind. 16, 102–109 (2024). https://doi.org/10.1134/S2070050424010070

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