Skip to main content
SpringerLink
Log in

Comparative Analysis of the Properties of Biocatalysts Based on Chymotrypsin Immobilized on Polysaccharide Supports

  • BIOCATALYSIS
  • Published:
Catalysis in Industry Aims and scope Submit manuscript

Abstract

The effect of the type of polysaccharide support for immobilization and encapsulation on the stability of chymotrypsin was studied. The synthesized biocatalysts were compared according to their proteolytic activity. The cellulose–chitosan composite was found to have the highest proteolytic activity equal to 192 units/g. Immobilization was found to slightly change the optimum temperature and pH of chymotrypsin, but they substantially grew toward higher temperatures and alkaline pH values. The greatest relative increase in the activity of immobilized chymotrypsin was observed when using the cellulose–chitosan composite. The activity of chymotrypsin changed by no more than 45–50% during storage of the cellulose–chitosan and cellulose–alginate composites for 24 months. According to the results of our study, the cellulose–chitosan composite was the optimum support for immobilization of chymotrypsin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

REFERENCES

  1. Khvostov, D.V., Konorov, E.A., Zakharova, M.V., and Kovalevskaya, Zh.I., Myasn. Ind., 2020, no. 11, pp. 32–37. https://doi.org/10.37861/2618-8252-2020-11-32-36

  2. Dos Santos Aguilar, J.G. and Sato, H.H., Food Res. Int., 2018, vol. 103, pp. 253–262. https://doi.org/10.1016/j.foodres.2017.10.044

    Article  CAS  Google Scholar 

  3. Hua, Y. and Nair, S., Biochim. Biophys. Acta, Mol. Basis Dis., 2015, vol. 1852, no. 2, pp. 195–208. https://doi.org/10.1016/j.bbadis.2014.04.032

    Article  CAS  Google Scholar 

  4. Vojcic, L., Pitzler, C., Korfer, G., Jakob, F., Martinez, R., and Maurer, K.-H., New Biotechnol., 2015, vol. 32, no. 6, pp. 629–634. https://doi.org/10.1016/j.nbt.2014.12.010

    Article  CAS  Google Scholar 

  5. Borovikova, L.N., Titova, A.V., Matveeva, N.A., and Pisarev, O.A., Russ. J. Phys. Chem. A., 2013, vol. 87, no. 6, pp. 998–1001. https://doi.org/10.1134/S0036024413060058

    Article  CAS  Google Scholar 

  6. Soldatova, L.S., Babich, O.O., and Prosekov, A.Yu., Pererab. Moloka, 2010, no. 10, pp. 56–58.

  7. Maksimova, Yu.G., Demakov, V.A., Maksimov, A.Yu., Ovechkina, G.V., and Kovalenko, G.A., Appl. Biochem. Microbiol., 2010, vol. 46, no. 4, pp. 379–384. https://doi.org/10.1134/S0003683810040022

    Article  CAS  Google Scholar 

  8. Martirosova, E.I., Nikolaev, Yu.A., Shanenko, E.F., Krupyanskii, Yu.F, Loiko, N.G., and El’-Registan, G.I., Khim. Tekhnol., 2007, no. 6, pp. 250–256.

  9. Soldatova, L.S. and Babich, O.O., Tekhn. Tekhnol. Pishch. Proizvod., 2010, no. 1, pp. 1242–1247.

  10. Singh, N., Mathur, A.S., Gupta, R.P., Barrow, C.J., Tuli, D.K., and Puri, M., Int. J. Biol. Macromol., 2021, vol. 168. pp. 572–590. https://doi.org/10.1016/j.ijbiomac.2020.12.004

    Article  CAS  Google Scholar 

  11. Kotel’nikova, N.E., Mikhailova, S.A., and Vlasova, E.N., Russ. J. Appl. Chem., 2007, vol. 80, no. 2, pp. 322–329. https://doi.org/10.1134/S1070427207020309

    Article  CAS  Google Scholar 

  12. Sarmento, B., Ribeiro, A., Veiga, F., and Sampaio, P., Pharm. Res., 2007, vol. 24, no. 12, pp. 2198–2206. https://doi.org/10.1007/s11095-007-9367-4

    Article  CAS  Google Scholar 

  13. Markvicheva, E.A., Kuptsova, S.V., Vikhoreva, G.A, and Mareeva, T.Yu., Abstract of Papers, Materialy shestoi mezhdunarodnoi konferentsii “Novye dostizheniya v issledovanii khitina i khitozana” (Proc. Sixth Int. Conf. “New Achievements in the Study of Chitin and Chitosan), Moscow, 2001, pp. 212–216.

  14. Dosadina, E.E., Savelyeva, E.E., and Belov, A.A., Process Biochem., 2018, vol. 64, no. 1, pp. 213–220. https://doi.org/10.1016/j.procbio.2017.10.002

    Article  CAS  Google Scholar 

  15. Efimtseva, E.A. and Chelpanova, T.I., Biotekhnologiya, 2017, vol. 33, no. 4, pp. 54–75. https://doi.org/10.21519/0234-2758-2017-33-4-54-75

    Article  Google Scholar 

  16. Bychkova, E.S., Sin, A.D., Belyakova, D.A., Kotova, Ya.S., and Lomovskii, I.O., Pishch. Prom-st’, 2021, no. 4, pp. 36–41. https://doi.org/10.24412/0235-2486-2021-4-0044

  17. Shkol’nikova, M.N. and Voronova, E.V., Food Ind., 2021, vol. 6, no. 2, pp. 90–98. https://doi.org/10.29141/2500-1922-2021-6-2-11

    Article  Google Scholar 

  18. Kamilov, F.Kh., Galimov, Sh.N., Agletdinov, E.F., Knyazeva, O.A., Abdullina, G.M., Karyagina, N.T., Baigil’dina, A.A., Valiev, A.G., Sagidullin, F.A., Kulagina, I.G., Kidrasova, R.S., Men’shikova, I.A., and Bikmetova, E.R., Biokhimicheskii praktikum: posobie dlya samostoyatel’noi auditornoi raboty studentov, obuchayushchikhsya po spetsial’nosti 20400.62–Biologiya, profil' Mikrobiologiya (Biochemical Workshop: a Manual for Independent Classroom Work of Students Studying in the specialty 20400.62—Biology, Microbiology), Ufa: GBOU VPO BGMU Minzdrava Rossii, 2014, part 2.

  19. Beloded, A.V., Khabibulina, N.V., and Lutsenko, N.G., Enzimologiya. Laboratornyi praktikum po biokhimii i molekulyarnoi biologii (Enzymology. Laboratory Workshop on Biochemistry and Molecular Biology), Moscow: RKhTU im. D.I. Mendeleeva, 2019.

  20. Toroptseva, A.M., Belogorodskaya, K.V., and Bondarenko, V.M., Laboratornyi praktikum po khimii i tekhnologii vysokomolekulyarnykh soedinenii (Laboratory Workshop on Chemistry and Technology of High-Molecular Compounds), Leningrad, 1972.

  21. Belov, A.A., Belova, E.N., and Filatov, V.N., Biomed. Khim., 2009, vol. 55, no. 1, pp. 61–67.

    CAS  Google Scholar 

  22. Raspopova, E.A. and Krasnoshtanova, A.A., Catal. Ind., 2016, vol. 8, no. 1, pp. 75–80. https://doi.org/10.1134/S2070050416010104

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Mendeleev University of Chemical Technology of Russia, 125047, Moscow, Russia

    A. A. Krasnoshtanova & A. D. Bezyaeva

Authors
  1. A. A. Krasnoshtanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. D. Bezyaeva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to A. A. Krasnoshtanova or A. D. Bezyaeva.

Additional information

Translated by L. Smolina

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Krasnoshtanova, A.A., Bezyaeva, A.D. Comparative Analysis of the Properties of Biocatalysts Based on Chymotrypsin Immobilized on Polysaccharide Supports. Catal. Ind. 14, 395–400 (2022). https://doi.org/10.1134/S2070050422040043

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S2070050422040043

Keywords:

Search

Navigation