Abstract
Controlled burning has been carried out to stimulate moderate ground fire in the Scots pine (Pinus sуlvestris L.) stand (age class I) in the Krasnoyarsk forest steppe at the beginning of the growing season. The structure of annual rings and trophic supply, as well as the cambial zone antioxidant system condition and the content of photosynthetic pigments in the needles of dominant and suppressed trees in the experimental and control plots, were studied 3 h, 5 days, and 2.5 months after the fire. The fire narrowed the annual ring width, since the number and size of early and late tracheids decreased. The primary reaction to pyrogenic heat shock (sharp reduction of chlorophyll content in needles and manifestation of oxidative stress in the stem cambial zone) was an elevated level of hydrogen peroxide and activity of most antioxidant enzymes and a reduction in the nonstructural carbohydrates pool. Restoration of the cambium function after the fire was promoted by an increased activity of amylase, invertase, and peroxidase.
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Aebi, H., Catalase, in Methods of Enzymatic Analysis, London: Verlag Chemie, 1974, vol. 2, pp. 673–684.
Anderson, J.V., Chevone, B.I., and Hess, J.L., Seasonal variation in the antioxidant system of eastern white pine needles: evidence for thermal dependence, Plant Physiol., 1992, vol. 98, no. 2, pp. 501–508.
Bakardjieva, N.T., Christov, K.N., and Christova, N.V., Effect of calcium and zinc on the activity and thermostability of superoxide dismutase, Biol. Plant, 2000, vol. 43, no. 1, pp. 73–78.
Bergmeyer, H.U., Enzymes as biochemical reagents, in Methods of Enzymatic Analysis, London: Verlag Chemie, 1974, vol. 1, pp. 425–522.
Blokhina, O., Virolainen, E., and Fagerstedt, K.V., Antioxidants, oxidative damage, and oxygen deprivative stress: a review, Ann. Bot., 2003, vol. 91, no. 2, pp. 179–194.
Chudinova, V.V., Alekseev, S.M., Zakharova, E.I., and Evstigneeva, R.P., Lipid peroxidation and mechanism of antioxidant activity of vitamin E, Bioorg. Khim., 1994, vol. 20, no. 10, pp. 1029–1046.
Dipierro, S. and Leonardis, S.D., The ascorbate system and lipid peroxidation in stored potato (Solanum tuberosum L.) tubers, J. Exp. Bot., 1997, vol. 48, no. 3, pp. 779–783.
Ducrey, M., Duhoux, F., Huc, R., and Rigolot, E., The ecophysiological and growth responses of Aleppo pine (Pinus halepensis) to controlled heating applied to the base of the trunk, Can. J. For. Res., 1996, vol. 26, no. 8, pp. 1366–1374.
Esterbauer, H. and Grill, D., Seasonal variation of glutathione and glutathione reductase in needles of Picea abies L., Plant Physiol., 1978, vol. 61, no. 1, pp. 119–121.
Hood, S.M., Cluck, D.R., Smith, S.L., and Ryan, K.C., Using bark char codes to predict post-fire cambium mortality, Fire Ecol., 2008, vol. 4, no. 1, pp. 57–73.
Humphreys, F.R. and Kelly, J., A method for determination of starch in wood, Anal. Chem. Acta, 1961, vol. 24, no. 1, pp. 66–70.
Isaev, A.S. and Girs, G.I., Vzaimodeistvie dereva i nasekomykh-ksilofagov (Interaction of a Tree and Insects-Xylophages), Novosibirsk: Nauka, 1975.
Gill, S.S. and Tuteja, N., Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants, Plant Physiol. Biochem., 2010, vol. 48, no. 12, pp. 909–930.
Kumar, G.N.M. and Knowles, N.R., Changes in lipid peroxidation and lipolytic and free-radical scavenging enzyme activities during aging and sprouting of potato (Solanum tuberosum) seed-tubers, Plant Physiol., 1993, vol. 102, no. 1, pp. 115–174.
Laketa, D., Bogdanovic, J., Kalauzi, A., and Radotic, K., Kinetic parameters for thermal inactivation of soluble peroxidase from needles of Serbian spruce Picea omorika (Pancic) Purkyne, Gen. Physiol. Biophys., 2009, vol. 28, no. 1, pp. 78–85.
Masyagina, O.V., Prokushkin, S.G., and Ivanova, G.A., Influence of fires on respiration intensity of the Scots pine trunk (Pinus silvestris L.), Khvoinye Borel’noi Zony, 2007, vol. 24, no. 1, pp. 82–90.
Melekhov, I.S., Vliyanie pozharov na les (Influence of the Wild Fires on Forest), Moscow: Goslestekhizdat, 1948.
Merzlyak, M.N., Active oxygen and oxidative processes in the plant cell membranes, Itogi Nauki Tekh., Ser.: Fiziol. Rast., 1989, vol. 6.
Milyutina, I.L., Biochemical adaptation of Scots pine to low temperatures, Extended Abstract of Cand. Sci. (Biol.) Dissertation, Krasnoyarsk: Inst. Forest, Sib. Branch, Russ. Acad. Sci., 1994.
Minocha, R., Turlapati, S.A., Long, S., and North, M., Fuel treatment effects on soil chemistry and foliar physiology of three coniferous species at the Teakettle experimental forest, California, USA, Trees, 2013, vol. 27, no. 4, pp. 1101–1113.
Mohamed, S.A., Al-Malki, A.L., and Kumosani, T.A., Partial purification and characterization of five a-amylases from a wheat local variety (Balady) during germination, Aust. J. Basic Appl. Sci., 2009, vol. 3, no. 3, pp. 1740–1748.
Pastori, G.M. and Trippi, V.S., Oxidative stress induces high rate of glutathione reductase synthesis in a drought-resistant maize strain, Plant Cell Physiol., 1992, vol. 33, no. 7, pp. 957–961.
Polle, A., Chakrabarti, K., Schürmann, W., and Renneberg, H., Composition and properties of hydrogen peroxide decomposing systems in extracellular and total extracts from needles of Norway spruce (Picea abies L.,Karst.), Plant Physiol., 1990, vol. 94, no. 1, pp. 312–319.
Potikha, T.S., Collins, C.C., Johnson, D.I., Delmer, D.P., and Levine, A., The involvement of hydrogen peroxide in the differentiation of secondary walls in cotton fibers, Plant Physiol., 1999, vol. 119, no. 3, pp. 849–858.
Putter, J., Peroxidases, in Methods of Enzymatic Analysis, London: Verlag Chemie, 1974, vol. 2, pp. 685–690.
Rieske, L.K., Wildfire alters oak growth, foliar chemistry, and herbivore, For. Ecol. Manage., 2002, vol. 168, nos. 1–3, pp. 91–99.
Shlyk, A.A., Determination of chlorophylls and carotenoids in the extracts of green leaves, in Biokhimicheskie metody v fiziologii rastenii (Biochemical Methods in the Plant Physiology), Moscow: Nauka, 1971, pp. 154–170.
Sudachkova, N.E., Metabolizm khvoinykh i formirovanie drevesiny (Metabolism of Conifers and Development of Wood), Novosibirsk: Nauka, 1977.
Sudachkova, N.E., Milyutina, I.L., and Romanova, L.I., Biokhimicheskaya adaptatsiya khvoinykh k stressovym uloviyam Sibiri (Biochemical Adaptation of Conifers to Stress Conditions), Novosibirsk: Geo, 2012.
Sudachkova, N.E., Milyutina, I.L., Romanova, L.I., Kosov, I.V., and Sobachkin, D.S., Influence of low wild fires on vitality and antioxidant resistance of the young plantations of Scots pine in Krasnoyarsk foreststeppe, Lesovedenie, 2015, no. 2, pp. 16–25.
Sudachkova, N.E., Milyutina, I.L., Romanova, L.I., and Semenova, G.P., The dynamics of reserve compounds and hydrolytic enzymes activity in the tissues of Pinus sylvestris L. and Larix sibirica Ledeb., Eurasian J. For. Res., 2004, vol. 7, no. 1, pp. 1–10.
Vaganov, E.A. and Shashkin, A.V., Rost i struktura godichnykh kolets khvoinykh (Growth and Structure of Annual Rings of Conifers), Novosibirsk: Nauka, 2000.
Varner, J.M., Putz, F.E., O’Brien, J.J., Hiers, J.K., Mitchell, R.J., and Gordon, D.R., Post-fire tree stress and growth following smoldering duff fires, For. Ecol. Manage., 2009, vol. 258, no. 11, pp. 2467–2474.
Velikova, V., Jordanov, I., and Edreva, A., Oxidative stress and some antioxidant system in acid-rain treated bean plants: protective role of exogenous polyamines, Plant Sci., 2000, vol. 151, no. 1, pp. 59–66.
Voznesenskii, V.L., Gorbacheva, G.I., Shtan’ko, T.P., and Filippova, L.A., Analysis of sugars by bleaching of Fehling’s solution, Fiziol. Rast., 1962, vol. 9, no. 2, pp. 255–266.
Wingsle, G., Mattson, A., Ekblad, A., Hällgren, J.-E., and Selstam, E., Activities of glutathione reductase and superoxide dismutase in relation to changes of lipids and pigments due to ozone in seedlings of Pinus sylvestris (L), Plant Sci., 1992, vol. 82, no. 2, pp. 167–178.
Zaprometov, M.N. and Shipilova, S.V., Phenylalanine ammonia-lyase and synthesis of phenolic compounds in corn seedlings, Fiziol. Rast., 1972, vol. 19, no. 3, pp. 489–503.
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Original Russian Text © N.E. Sudachkova, L.I. Romanova, N.V. Astrakhantseva, M.V. Novoselova, I.V. Kosov, 2016, published in Sibirskii Ekologicheskii Zhurnal, 2016, No. 5, pp. 739–749.
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Sudachkova, N.E., Romanova, L.I., Astrakhantseva, N.V. et al. Stress reactions of Scots pine trees to injuring by ground fire. Contemp. Probl. Ecol. 9, 608–616 (2016). https://doi.org/10.1134/S1995425516050152
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DOI: https://doi.org/10.1134/S1995425516050152