Abstract
Dynamics of bacterial plankton community of eutrophic water reservoir in laboratory microecosystems with amino acid lysine was studied using PCR-DGGE technique. The addition of lysine to the microecosystems resulted in changes in the composition of the bacterial plankton in summer; in particular, a number of Lys1 and Lys2 species (genotypes) that consume this amino acid grew fast in the bacterial community. The plank tonic bacterial communities did not respond to the addition of lysine in spring and late summer. The obtained data confirm the hypothesis for the narrow specialization of bacterial plankton species to the consumption of individual organic substances.
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Giovannoni, S. and Stingl, U., Molecular Diversity and Ecology of Microbial Plankton, Nature, 2005, vol. 437, pp. 343–348.
Fuhrman, J.A. and Steele, J.A., Community Structure of Marine Bacterioplankton: Patterns, Networks, and Relationships to Function, Aquat. Microb. Ecol., 2008, vol. 53, pp. 69–81.
Mou, X., Sun, S., Edwards, R.A., Hodson, R.E., and Moran, M.A, Bacterial Carbon Processing by Generalist Species in the Coastal Ocean, Nature, 2008, vol. 451, pp. 708–711.
Buck, U., Grossart, H.-P., Amann, R., and Pernthaler, J., Substrate Incorporation Patterns of Bacterioplankton Populations in Stratified and Mixed Waters of a Humic Lake, Environ. Microbiol., 2009, vol. 11, no. 7, pp. 1854–1865.
Ouverney, C.C. and Fuhrman, J.A, Marine Planktonic Archaea Take Up Amino Acids, Appl. Environ. Microbiol, 2000, vol. 66, pp. 4829–4833.
Schäfer, H., Bernard, L., Courties, C., Lebaron, P., Servais, P., Pukall, R., Stackebrandt, E., Troussellier, M., Guindulain, T., Vives-Rego, J., and Muyzer, G., Microbial Community Dynamics in Mediterranean Nutrient-Enriched Seawater Mesocosms: Changes in the Genetic Diversity of Bacterial Populations, FEMS Microbiol. Ecol., 2001, vol. 34, pp. 243–253.
Carlson, C.A., Giovannoni, S.J., Hansell, D.A., Goldberg, S.J., Parsons, R., Otero, M.P., Vergin, K., and Wheeler, B.R., Effect of Nutrient Amendments on Bacterioplankton Production, Community Structure, and DOC Utilization in the Northwestern Sargasso Sea, Aquat. Microb. Ecol., 2002, vol. 30, pp. 19–36.
Zubkov, M.V., Differential Microbial Uptake of Dissolved Amino Acids and Amino Sugars in Surface Waters of the Atlantic Ocean, J. Plankton Res., 2008, vol. 30, no. 2, pp. 211–220.
Øvreås, L., Bourne, D., Sandaa, R.A., Casamayor, E.O., Benlloch, S., Goddard, V., Smerdon, G., Heldal, M., and Thingstad, T.F., Response of Bacterial and Viral Communities to Nutrient Manipulations in Seawater Mesocosms, Aquat. Microb. Ecol., 2003, vol. 31, pp. 109–121.
Rosenstock, B. and Simon, M., Sources and Sinks of Dissolved Free Amino Acids and Protein in a Large and Deep Mesotrophic Lake, Limnol. Oceanogr., 2001, vol. 46, no. 3, pp. 644–654.
Kolmakova, O.V. and Trusova, M.Yu., The Consumption of Amino Acids by the Uncultivated Bacterial Plankton of the Eutrophic Reservoir, Sib. J. Ecol., 2011, no. 1, pp. 13–21.
Kalachova, G.S., Kolmakova, A.A., Gladyshev, M.I., Kravchuk, E.S., and Ivanova, E.A., Seasonal Dynamics of Amino Acids in Two Small Siberian Reservoirs Dominated by Prokaryotic and Eukaryotic Phytoplankton, Aquat. Ecol., 2004, vol. 38, pp. 3–15.
Fox, J.M., Lawrence, A.M., and Li-Chang, E., Dietary Requirement for Lysine by Juvenile Penaeus Vannamei Using Intact and Free Amino Acids Sources, Aquaculture, 1995, vol. 131, pp. 279–290.
Schuhmacher, A., Wax, C., and Gropp, J. M., Plasma Amino Acids in Rainbow Trout (Oncorhynchus Mykiss) Fed Intact Protein or a Crystalline Amino Acid Diet, Aquaculture, 1995, vol. 151, pp. 15–28.
Gladyshev, M.I., Sushchik, N.N., Kolmakova, A.A., Kalachova, G.S., Kravchuk, E.S., Ivanova, E.A., and Makhutova, O.N., Seasonal Correlations of Elemental and Omega3 PUFA Composition of Seston and Dominant Phytoplankton Species in a Eutrophic Siberian Reservoir, Aquat. Ecol., 2007, vol. 41, pp. 9–23.
Gladyshev, M.I., Sushchik, N.N., Makhutova, O.N., Dubovskaya, O.P., Kravchuk, E.S., Kalachova, G.S., and Khromechek, E.B., Correlations Between Fatty Acid Composition of Seston and Zooplankton and Effects of Environmental Parameters in a Eutrophic Siberian Reservoir, Limnologica, 2010, vol. 40, pp. 343–357.
Gladyshev, M.I., Sushchik, N.N., Anishchenko, O.V., Makhutova, O.N., Kolmakov, V.I., Kalachova, G.S., Kolmakova, A.A., and Dubovskaya, O.P., Efficiency of Transfer of Essential Polyunsaturated Fatty Acids versus Organic Carbon from Producers to Consumers in a Eutrophic Reservoir, Oecologia, 2011, vol. 165, pp. 521–531.
Kravchuk, E.S., Ivanova, E.A., and Gladyshev, M.I., Spatial Distribution of Resting Stages (Akinetes) of the Cyanobacteria Anabaena Flosaquae in Sediments and its Influence on Pelagic Populations, Mar. Freshwater Res., 2011, vol. 62, pp. 450–461.
Gladyshev, M.I., Sushchik, N., Kalachova, G.S., and Shchur, L.A., The Effect of Algal Blooms on the Disappearance of Phenol in a Small Forest Pond, Water Res., 1998, vol. 32, no. 9, pp. 2769–2775.
Sushchik, N.N., Gladyshev, M.I., Kalacheva, G.S., and Plaksina, I.V., Changes in the Biomass of the Algae and the Composition of Extracellular Free Fat Acids in the Experimental Microecosystems, Izv. Akad. Nauk SSSR, Ser. Biol., 1998, no. 6, pp. 738–744.
Gladyshev, M.I., The Experimental Ecosystems and Their Application for Investigation of Biodegradation of Easily Oxidable Toxic Agents in Pelagic Zone (Review), Gidrobiol. Zh., 1992, vol. 28, no. 5, pp. 68–77.
Böstrom, K.H., Simu, K., Hagstrom, A., and Riemann, L., Optimization of DNA Extraction for Quantitative Marine Bacterioplankton Community Analysis, Limnol. Oceanogr.: Methods, 2004, vol. 2, pp. 365–373.
Schauer, M., Massana, R., and Pedros-Alio, C., Spatial Differences in Bacterioplankton Composition along the Catalan Coast (NW Mediterranean) Assessed by Molecular Fingerprinting, FEMS Microbiol. Ecol., 2000, vol. 33, pp. 51–59.
Hill, T.C.J., Walsh, K.A., Harris, J.A., and Moffett, B.F., Using Ecological Diversity Measures with Bacterial Communities, FEMS Microbiol. Ecol., 2003, vol. 43, pp. 1–11.
Dunbar, J., Takala, S., Barns, S.M., Davis, J.A., and Kuske, C.R., Levels of Bacterial Community Diversity in Four Arid Soils Compared by Cultivation and 16S rRNA Gene Cloning, Appl. Environ. Microbiol., 1999, vol. 65, pp. 1662–1669.
Jaffers, J., Vvedenie v sistemii analis: primenenie v ekologii (An Introduction to Systems Analysis: with Ecological Applications), Moscow: Mir, 1981.
Lebaron, P., Servais, P., Troussellier, M., Courties, C., Muyzer, G., Bernard, L., Schäfer, H., Pukall, R., Stackebrandt, E., Guindulain, T., and Vives-Rego, J., Microbial Community Dynamics in Mediterranean Nutrient-Enriched Seawater Mesocosms: Changes in Abundances, Activity and Composition, FEMS Microbiol. Ecol., 2001, vol. 34, pp. 255–266.
Gladyshev, M.I., Sushchik, N.N., Dubovskaya, O.P., Makhutova, O.N., and Kalachova, G.S., Growth Rate of Daphnia Feeding on Seston in a Siberian Reservoir: the Role of Essential Fatty Acid, Aquat. Ecol., 2008, vol. 42, pp. 617–627.
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Original Russian Text © M.Yu. Trusova, O.V. Kolmakova, M.I. Gladyshev, 2012, published in Sibirskii Ekologicheskii Zhurnal, 2012, No. 4, pp. 529–539.
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Trusova, M.Y., Kolmakova, O.V. & Gladyshev, M.I. Seasonal features of consumption of lysine by uncultivated bacterial plankton of Eutrophic water reservoir. Contemp. Probl. Ecol. 5, 391–398 (2012). https://doi.org/10.1134/S1995425512040154
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DOI: https://doi.org/10.1134/S1995425512040154