Skip to main content
Log in

A study of biomedical properties of hydrogels based on recombinant spidroin after their sterilization

  • Published:
Polymer Science Series D Aims and scope Submit manuscript

Abstract

A comparative analysis of rheological, organoleptic, physical and chemical properties of hydrogels was performed and their biosafety was examined in cell cultures before and after radiation and tyndallization sterilization. It was shown that the radiation sterilization does not destabilize the protein molecules of hydrogels at exposure doses less than 15 kGy, thereby not changing their physical, chemical and biomedical properties and, hence, the operational characteristics of products based on hydrogels.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. M. Widhe, H. Bysell, S. Nystedt, I. Schenning, et al., “Recombinant spider silk as matrices for cell culture,” Biomaterials 31, 9575–9585 (2010).

    Article  CAS  Google Scholar 

  2. B. Kundu, N. E. Kurland, V. K. Yadavalli, and S. C. Kundu, “Isolation and processing of silk proteins for biomedical applications,” Int. J. Biol. Macromol. 70, 70–77 (2014).

    Article  CAS  Google Scholar 

  3. V. G. Bogush, A. Yu. Sazykin, L. I. Davydova, V. V. Martirosyan, K. V. Sidoruk, A. V. Glazunov, R. I. Akishina, N. A. Shmatchenko, and V. G. Debabov, “Preparation, purification and spinning of recombinant analog of spidroin 1,” Biotekhnologiya 4, 3–12 (2006).

    Google Scholar 

  4. V. G. Bogush, O. S. Sokolova, L. I. Davydova, D. V. Klinov, K. V. Sidoruk, N. G. Esipova, T.V.Neretina, I. A. Orchanskyi, V. Yu. Makeev, V. G. Tumanyan, K. V. Shayjtan, V. G. Debabov, and M. P. Kirpichnikov, “A novel model system for design of biomaterials, based on recombinant analogs of spider silk protein,” J. Neuroimmune Pharmacol. 4 (1), 17–27 (2009).

    Article  Google Scholar 

  5. V. G. Bogush, L. I. Davydova, M. M. Moisenovich, K.V. Sidoruk, A. Yu. Arkhipova, D. G. Kozlov, I. I. Agapov, M. P. Kirpichnikov, and V. G. Debabov, “Biodegradable microand macro cellular carriers on the basis of recombinant spidroin,” Biotekhnologiya, No. 1, 52–61 (2014).

    Google Scholar 

  6. M. J. Garle, J. H. Fentem, and J. R. Fry, “In vitro cytotoxicity tests for the prediction of acute toxicity in vivo,” Toxicol. In Vitro 8, 1303–1313 (1994).

    Article  CAS  Google Scholar 

  7. Yu. K. Abaev, Surgeon Directory. Wounds and Wound Infections (Feniks, Rostov-on-Don, 2006) [in Russian].

    Google Scholar 

  8. GOST (State Standard) 29188.0–91: Articles of perfumery and cosmetics. Acceptance rules, sampling and methods of organoleptic tests (1991).

  9. M. Zholi, Physical Chemistry. Protein Denaturation (Mir, Moscow, 1968) [in Russian].

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to O. A. Legon’kova.

Additional information

Original Russian Text © O.A. Legon’kova, I.P. Savchenkova, M.S. Belova, A.I. Korotaeva, L.I. Davydova, V.G. Bogush, 2016, published in Vse Materialy. Entsiklopedicheskii Spravochnik, 2016, No. 1, pp. 38–43.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Legon’kova, O.A., Savchenkova, I.P., Belova, M.S. et al. A study of biomedical properties of hydrogels based on recombinant spidroin after their sterilization. Polym. Sci. Ser. D 9, 219–222 (2016). https://doi.org/10.1134/S199542121602012X

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S199542121602012X

Keywords

Navigation