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The Influence of Abiotic Factors on the Structural and Functional Characteristics of the Diatom Algae Cerataulina pelagica (Сleve) Hendey

  • PHYTOPLANKTON, PHYTOBENTHOS, PHYTOPERIPHYTON
  • Published:
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Abstract

Using the electron microscopy method, the species identity of the diatom Cerataulina pelagica, isolated in pure culture from the coastal waters of the Black Sea in September 2021, has been confirmed. The range of optimal temperature values for the development of this species is identified. The impact of light and biogenic substances on its main structural and functional characteristics has been studied. The acclimation of C. pelagica to various light intensities is carried out by changing the efficiency of photosystem II, the C/Chl a ratio, and the specific growth rate. The morphometric parameters of cells (volume, surface area, and specific surface area) change slightly in the studied light range: 8.5–510 μE/(m2 s). The transfer of C. pelagica cells, which have the maximum intracellular pool of nutrients, to seawater depleted in nutrients causes a rapid increase in the C/Chl a ratio, as well as a decrease in the efficiency of photosystem II, the relative rate of electron transport, and the specific growth rate. A high degree of toxicity with copper ion in low concentrations in relation to the studied species is shown.

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REFERENCES

  1. Ajani, P.A., Davies, C.H., Eriksen, R.S., et al., Global warming impacts micro-phytoplankton at a long-term Pacific Ocean coastal station, Front. Mar. Sci., 2020, vol. 7, p. 576011. https://doi.org/10.3389/fmars.2020.576011

    Article  Google Scholar 

  2. Akimov, A.I. and Solomonova, E.S., Characteristics of growth and fluorescence of certain types of algae during acclimation to different temperatures under culture conditions, Oceanology, 2019, vol. 59, no. 3, p. 316. https://doi.org/10.1134/S0001437019030019

    Article  CAS  Google Scholar 

  3. Berger, V.Ya., Mityaev, M.V., and Sukhotin, A.A., Practical wet oxidation experiment to determine concentrations of particulate organic matter in seawater, Oceanology, 2016, vol. 56, p. 307. https://doi.org/10.7868/S0030157416020015

    Article  CAS  Google Scholar 

  4. Bryantseva, Yu.V., Lyakh, A.M., and Sergeeva, A.V., Raschet ob”emov i ploshchadei poverkhnosti odnokletochnykh vodoroslei Chernogo morya (Calculation of Volumes and Surface Areas of Single-Celled Algae of the Black Sea), Preprint of Inst. Biol. South. Seas, Sevastopol, 2005.

  5. Cruz, S. and Serôdio, J., Relationship of rapid light curves of variable fluorescence to photoacclimation and non-photochemical quenching in a benthic diatom, Aquat. Bot., 2008, vol. 88, p. 256.

    Article  CAS  Google Scholar 

  6. Edwards, K.F., Klausmeier, C.A., and Litchman, E., Evidence for a three-way trade-off between nitrogen and phosphorus competitive abilities and cell size in phytoplankton, Ecology, 2011, vol. 92, p. 2085. https://doi.org/10.1890/11-0395.1

    Article  PubMed  Google Scholar 

  7. Finenko, Z.Z., Stelmakh, L.V., Mansurova, I.M., et al., Seasonal dynamic of structural and functional characteristics of the phytoplankton community in the Sevastopol Bay, Sist. Kontrolya Okruzh. Sredy, 2017, vol. 29, p. 73. https://doi.org/10.33075/2220-5861-2017-3-73-82

    Article  Google Scholar 

  8. Guillard, R.R.L. and Ryther, J.H., Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran., Can. J. Microbiol., 1962, vol. 8, p. 229.

    Article  CAS  PubMed  Google Scholar 

  9. Hernández-Becerril, D.U., Morphology of two species of the marine planktonic diatom genus Cerataulina H. Peragallo ex Schütt (Bacillariophyta) from the Tropical Mexican Pacific, including a new record for the area, Bol. Inst. Oceanogr., 2020, vol. 59, no. 1, p. 9.

    Google Scholar 

  10. Korablina, I.V., Barabashin, T.O., Gevorkyan, Zh.V., et al., The dynamics of the distribution of heavy metals in the water column of the north-eastern part of the Black Sea after 2000, Tr. Vseross. Nauchno-Issled. Inst. Rybn. Khoz. Okeanogr., 2021, vol. 183, p. 96. https://doi.org/10.36038/2307-3497-2021-183-96-112

    Article  Google Scholar 

  11. Kvíderová, J. and Lukavský, J., The cultivation of Phaeodactylum tricornutum in crossed gradients of temperature and light, Algol. Stud., 2003, vol. 110, no. 1, p. 67. https://doi.org/10.1127/1864-1318/2003/0110-0067

    Article  Google Scholar 

  12. MacIntyre, H.L., Kana, T.M., Anning, T., et al., Photoacclimation of photosynthesis irradiance response curves and photosynthetic pigments in microalgae and cyanobacteria, J. Phycol., 2002, vol. 38, p. 17.

    Article  Google Scholar 

  13. Mann, D.G., The species concept in diatoms, Phycologia, 1999, vol. 38, no. 6, p. 437. https://doi.org/10.2216/i0031-8884-38-6-437.1

    Article  Google Scholar 

  14. Marañón, E., Cermeño, P., Lopez-Sandoval, D.C., et al., Unimodal size scaling of phytoplankton growth and the size dependence of nutrient uptake and use, Ecol. Lett., 2013, vol. 16, p. 371. https://doi.org/10.1111/ele.12052

    Article  PubMed  Google Scholar 

  15. Mikaelyan, A.S., Kubryakov, A.A., Silkin, V.A., et al., Regional climate and patterns of phytoplankton annual succession in the open waters of the Black Sea, Deep Sea Res., 2018, vol. 142, p. 44. https://doi.org/10.1016/j.dsr.2018.08.00

    Article  CAS  Google Scholar 

  16. Moncheva, S., Gotsis-Skretas, O., Pagou, K., et al., Phytoplankton Blooms in Black Sea and Mediterranean Coastal Ecosystems Subjected to Anthropogenic Eutrophication: Similarities and Differences, Estuarine, Coastal Shelf Sci., 2001, vol. 53, p. 281. https://doi.org/10.1006/ecss.2001.0767

    Article  CAS  Google Scholar 

  17. Moreno-Garrido, I., Lubián, L.M., and Soares, A.M.V.M., Influence of cellular density on determination of EC50 in microalgal growth inhibition tests, Ecotoxicol. Environ. Saf., 2000, vol. 47, p. 112116. https://doi.org/10.1006/eesa.2000.1953

    Article  CAS  Google Scholar 

  18. Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. JGOFS Report Nr. 19, vi+170 pp. Reprint of the IOC Manuals and Guides No. 29. UNESCO. 1994. https://hdl.handle.net/11329/220.

  19. Raven, J.A., The role of vacuoles, New Phytol., 1987, vol. 106, no. 3, p. 357.

    Article  Google Scholar 

  20. Schreiber, V., Dersch, J., Puzik, K., et al., The central vacuole of the diatom Phaeodactylum tricornutum: identification of new vacuolar membrane proteins and of a functional Di-leucine-based targeting motif, Protist, 2017, vol. 168, no. 3, p. 271. https://doi.org/10.1016/j.protis.2017.03.001

    Article  CAS  PubMed  Google Scholar 

  21. Sherr, E.B. and Sherr, B.F., Heterotrophic dinoflagellates: a significant component of microzooplankton biomass and major grazers of diatoms in the sea, Mar. Ecol. Prog. Ser., 2007, vol. 352, p. 187. https://doi.org/10.3354/meps07161

    Article  Google Scholar 

  22. Shoman, N.Yu., The dynamics of the intracellular contents of carbon, nitrogen, and chlorophyll a under conditions of batch growth of the diatom Phaeodactylum tricornutum (Bohlin, 1897) at different light intensities, Russ. J. Mar. Biol., 2015, vol. 41, no. 5. p. 324. https://doi.org/10.1134/S1063074015050132

    Article  CAS  Google Scholar 

  23. Smetacek, V., Diatoms and the ocean carbon cycle, Protist, 1999, vol. 150, no. 1, p. 25. https://doi.org/10.1016/S1434-4610(99)70006-4

    Article  CAS  PubMed  Google Scholar 

  24. Sommer, U., Charalampous, E., Genitsaris, S., et al., Costs, benefits and taxonomic distribution of phytoplankton body size, J. Plankton Res., 2017, vol. 39, p. 494. https://doi.org/10.1093/plankt/fbw071

    Article  CAS  Google Scholar 

  25. Stelmakh, L.V., Features of the structural and functional characteristics of the diatom alga Pseudosolenia calcar-avis, Inland Water Biol., 2022, vol. 15, no. 3, pp. 315–323. https://doi.org/10.1134/S1995082922030154

    Article  Google Scholar 

  26. Stelmakh, L.V. and Georgieva, E.Yu., Microzooplankton: The trophic role and involvement in the phytoplankton loss and bloom-formation in the Black Sea, Turk. J. Fish. Aquat. Sci., 2014, vol. 14, p. 955. https://doi.org/10.4194/1303-2712-v14_4_15

    Article  Google Scholar 

  27. Stelmakh, L. and Kovrigina, N., Phytoplankton growth rate and microzooplankton grazing under conditions of climatic changes and anthropogenic pollution in the coastal waters of the Black Sea (Sevastopol Region), Water, 2021, vol. 13, no. 22, p. 3230. https://doi.org/10.3390/w13223230

    Article  CAS  Google Scholar 

  28. Stelmakh, L.V., Gubanov, V.I., and Babich, I.I., Seasonal variations of phytoplankton growth rate and its limitation by nutrients in coastal waters of the Black Sea near Sevastopol, Morsk. Ekol. Zh., 2004, vol. 3, no. 4, p. 55.

    Google Scholar 

  29. Tomas, C.R., Identifying Marine Diatoms and Dinoflagellates, New York: Acad. Press, 1997.

    Google Scholar 

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ACKNOWLEDGMENTS

I thank I.I. Babich and E.O. Verezubova (staff at the Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences) for their invaluable assistance in this study, as well as V.N. Lishaev, lead engineer at the Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, for conducting the electron microscopy.

Funding

This work was carried out based on the State Task of the Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, “Functional, Metabolic and Toxicological Aspects of the Existence of Hydrobionts and Their Populations in Biotopes with Different Physicochemical Regimes” (no. 121041400077-1).

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  1. Kovalevsky Institute of Biology of the Southern Seas, Russian Academy of Sciences, Sevastopol, Russia

    L. V. Stelmakh

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Correspondence to L. V. Stelmakh.

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Translated by V. Mittova

Abbreviations: Chl a, chlorophyll a; PAR, photosynthetically active radiation; Pheo a, pheophytin a; ETR, electron transport rate; Fm, Fv. maximum and variable fluorescence of dark-adapted samples; F 'm, Fv, maximum and variable fluorescence of samples adapted to light; Fv/Fm, maximum efficiency of photosystem II; F  'v/F  'm, efficiency of photosystem II at different light intensities; l, light intensities; and OD750, optical density at a wavelength of 750 nm.

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Stelmakh, L.V. The Influence of Abiotic Factors on the Structural and Functional Characteristics of the Diatom Algae Cerataulina pelagica (Сleve) Hendey. Inland Water Biol 16, 209–218 (2023). https://doi.org/10.1134/S1995082923020207

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