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Longitudinal River Survey of Migratory Fish Larvae and Juveniles by Secure Environmental DNA Field Sampling

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Abstract

Ayu Plecoglossus altivelis altivelis is a common osmerid freshwater fish distributed in Northeast Asia. Its annual life history allows discriminating 2 forms such as the amphidromous form migrating between river and sea and landlocked form migrating between river and lake or dam reservoir. In the Kando River basin, western Japan, ayu has been one of important fisheries resources, but its catch sustained at a low level after a drastic decline since 2000. This study was undertaken to perform environmental DNA (eDNA) survey for longitudinal distribution of ayu larvae and juveniles in the Kando River along approximately 60 km mainstream length. During the migration season in June 2019 and 2020, ayu juvenile eDNA was detected over the entire area of the Shidzumi Dam Reservoir in the middle reach of the river. During the spawning season in November 2019 and 2020, ayu larva eDNA was detected along approximately 13 km upstream flowing into the dam reservoir. These results first verify the landlocked form migrating between the dam reservoir and its upstream, since the dam blocks downstream migration. Ayu larva eDNA was also detected along approximately 45 km downstream of the dam to river mouth during the spawning season in 2019 and 2020. This result indicates the amphidromous form widely spread in the middle and lower reaches of the river. In addition, our data demonstrate successful reproduction of the both forms of ayu for two consecutive years in the Kando River.

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ACKNOWLEDGMENTS

Authors are indebted to R. Shinohara, Wesco Co., for help in water collection in the Shidzumi Dam Reservoir.

Funding

This study was supported in part by a grant (no. B4R31001) from Ministry of Land, Infrastructure and Transport of Japan to FA in Fisheries Ecosystem Project Center, Shimane University.

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Authors and Affiliations

  1. Fisheries Ecosystem Project Center, Shimane University, Matsue, Shimane, Japan

    F. Aranishi & T. Tanaka

  2. Institute of Agricultural and Life Sciences, Shimane University, Matsue, Shimane, Japan

    F. Aranishi

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  1. F. Aranishi
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  2. T. Tanaka
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Correspondence to F. Aranishi.

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Aranishi, F., Tanaka, T. Longitudinal River Survey of Migratory Fish Larvae and Juveniles by Secure Environmental DNA Field Sampling. Inland Water Biol 15, 62–67 (2022). https://doi.org/10.1134/S1995082922010035

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