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Influence of Moderate Hyperosmotic Stress on Ultrastructure and Indicators of Energy Metabolism of Chlorella vulgaris (Chlorophyta)

  • PHYTOPLANKTON, PHYTOBENTHOS, AND PHYTOPERIPHYTON
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Abstract

A comparison of the response of freshwater alga Chlorella vulgaris Beijer. on a quantitatively identical change in tonicity of growth medium by different osmotics (NaCl and sucrose) has been studied. Upon an increase in growth-medium osmotic potential (regardless the nature of osmoticum), increased thermogenesis and decreased oxygen absorption by Chlorella is observed first. The difference in the reaction of Chlorella to equiosmotic concentrations of NaCl and sucrose is revealed. Upon an increased salt background, thermogenesis and photosynthetic activity decreases in comparison with the control and the respiration rate is close to the control. In the presence of sucrose, the culture has a higher level of thermogenesis, respiration, and photosynthesis. Sodium chloride does not cause significant changes in ultrastructure of Chlorella and does not lead to significant changes in the diffusional decay of water magnetization in comparison with the control. Under sucrose stress, a change in the cell shape indicating cytorhysis is revealed. The effective water-diffusion coefficient (according to NMR data) decreases, which also indicates the reduction of cell sizes. Sucrose affects Chlorella as a nonpenetrating osmotically active substance. Under moderate hyperosmotic stress, the toxicity of NaCl is a smaller problem for freshwater microalga Chlorella than the partial dehydration caused by sucrose.

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ACKNOWLEDGMENTS

We are grateful to the Collective Spectro-Analytical Center for Physicochemical Studies of the Structure, Properties, and Composition of Substances and Materials, Kazan Scientific Center, Russian Academy of Sciences, for providing an electron microscope.

Funding

This study was carried out as part of a state task of the Kazan Scientific Center of the Russian Academy of Sciences.

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Authors and Affiliations

  1. Kazan Institute of Biochemistry and Biophysics, Federal Research Center Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russia

    A. J. Alyabev, I. N. Andreyeva, A. A. Ponomareva, V. V. Salnikov & M. A. Suslov

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  1. A. J. Alyabev
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  2. I. N. Andreyeva
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  3. A. A. Ponomareva
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  4. V. V. Salnikov
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  5. M. A. Suslov
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Correspondence to I. N. Andreyeva.

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This article does not contain any studies involving animals performed by any of the authors.

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Translated by V. Mittova

Abbreviations: NMR, nuclear magnetic resonance; Deff, mean effective water diffusion coefficient.

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Alyabev, A.J., Andreyeva, I.N., Ponomareva, A.A. et al. Influence of Moderate Hyperosmotic Stress on Ultrastructure and Indicators of Energy Metabolism of Chlorella vulgaris (Chlorophyta). Inland Water Biol 13, 425–433 (2020). https://doi.org/10.1134/S1995082920030025

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  • DOI: https://doi.org/10.1134/S1995082920030025

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