Abstract
Cultured hydrocarbon-oxidizing bacteria have been isolated for the first time from bacterioplankton in urban Lake Beloye (Moscow). The taxonomic positions of two bacterial strains (2012B and 2012C) isolated from this lake have been determined. Lipids of the strain 2012B comprise C14:0–C19:0 fatty acids, the most abundant of them being C15:0 (54%), C16:0 (17%), C17:0 (10%), and 10-methyl С18:0 (3.5%). Lipids of the strain 2012C comprise C14:0–C19:0 fatty acids, the most abundant of them being C15:0 (45%), C16:0 (32%), and C17:0 (9%). A phylogenetic analysis of strain 2012B is performed using the nucleotide sequences of the 16S rRNA (KP779654.1) and alkB (KR422620.1) genes and the strain is identified as a typical member of the genus Rhodococcus spp. (Actinobacteria, Nocardiaceae). The combination of molecular identification and analysis of biochemical and physiological properties makes it possible to identify strain 2012B as Rhodococcusqingshengii 2012B. A phylogenetic analysis of strain 2012C is performed using the nucleotide sequences of 16S rRNA (MG966152) and shows the highest identity (99.57%) of strain 2012С with Pseudomonas psychrotolerans and Pseudomonas oryzihabitans
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ACKNOWLEDGMENTS
We thank R.A. Sidorov (Institute of Plant Physiology, Russian Academy of Sciences) for assistance in determining the fatty acid composition.
Funding
This work was performed as part of the project “Physiological Ecology of Microorganisms of Aquatic Ecosystems” (State Order No. AAAA-A16-116021660041-4).
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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
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Translated by E. Makeeva
Abbreviations: PCR, polymerase chain reaction; HOB, hydrocarbon-oxidizing bacteria
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Kravzova, T.R., Ilinsky, V.V., Lazebnaya, I.V. et al. Hydrocarbon-Oxidizing Bacteria from Urban Lake Beloye (Moscow): Identification and Phylogenetic Analysis. Inland Water Biol 13, 178–185 (2020). https://doi.org/10.1134/S1995082920020236
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DOI: https://doi.org/10.1134/S1995082920020236