Abstract
The effects of phenol and its derivatives (4-chlorophenol, 4-nirophenol and 2,4-dinitrophenol) on the activity of peptidases in five species of fish differing in feeding modes have been studied. The power of the effects depends on the fish species and localization of the enzyme (mucosa or chyme). As a rule, in the in vitro conditions the phenols in concentrations of 0.06−0.5 μM/L considerably decreases activities of the intestinal peptidases in bream; silver bream; and, especially, in pike. In some cases, phenol and its derivatives induce an inconsiderable increase in peptidase activities. In zander and perch, in fact, no changes in the enzymatic activity under impact of phenols are observed. It is suggested that the effect of phenol and its derivatives depends to a high extent on the species specificity of peptidases: in the fish of fam. Percidae, the enzymes are relatively tolerant; in fam. Cyprinidae and Esocidae, they are sensitive to the studied toxicants.
Similar content being viewed by others
References
Alabaster, Dzh. and Lloid, R., Kriterii kachestva vody dlya presnovodnykh ryb (Water Quality Criteria for Freshwater Fishes), Moscow: Legk. Pishch. Prom-st’, 1984.
Zaprometov, M.N., Osnovy biokhimii fenol’nykh soedinenii (Basics of Biochemistry of Phenolic Compounds), Moscow: Vyssh. shk., 1974.
Ivanova, M.N., Polovkova, S.N., Kiyashko, V.I., and Bakanov, A.I., Foraging and trophic relationships of fishes in the Volga cascade reservoirs, in Teoreticheskie aspekty rybokhozyaistvennykh issledovanii vodokhranilishch (Theoretical Aspects of Fishery Studies of Reservoirs), Leningrad: Nauka, 1978, pp. 55–77.
Kuz’mina, V.V., Gracheva, E.L., Tarleva, A.F., and Tazhimuratova, U.Zh., Influence of phenol and its derivatives on the activity of hemoglobin-lytic proteases of intestinal mucosa and chyme in fishes of different species, in Antropogennoe vliyanie na vodnye organizmy i ekosistemy: Mater. V vseros. konf. (Anthropogenic Impact on Aquatic Organisms and Ecosystems: Proc. V. Conf.), Yaroslavl: Filigran’, 2014, part 2, pp. 62–66.
Luk’yanenko, V.I., Obshchaya ikhtiotoksikologiya (General Fish Toxicology), Moscow: Legk. Pishch. Prom-st’, 1983.
Maistrenko, V.N. and Klyuev, N.A., Ekologo-analiticheskii monitoring stoikikh organicheskikh zagryaznitelei (Ecological and Analytical Monitoring of Persistent Organic Pollutants), Moscow: Binom. Lab. Znanii, 2004.
Matei, V.E., Impact of subtoxic concentrations of phenol on the conditioned reflex activity of guppies, Gidrobiol. Zh., 1970, vol. 6, no. 3, pp. 100–103.
Nemova, N.N. and Vysotskaya, R.U., Biokhimicheskaya indikatsiya sostoyaniya ryb (Biochemical Indication of Fish State), Moscow: Nauka, 2004.
Orlov, D.S., Sadovnikova, L.K., and Lozanovskaya, I.N., Ekologiya i okhrana biosfery pri khimicheskom zagryaznenii (Ecology and Protection of the Biosphere Under Chemical Contamination), Moscow: Vyssh. Shk., 2002.
Poddubnyi, A.G., Ekologicheskaya topografiya populyatsii ryb v vodokhranilishchakh (Environmental Topography of Fish Populations in Reservoirs), Leningrad: Nauka, 1971.
Romanenko, V.I., Zakharova, L.I., Romanenko, V.A., et al., Water quality assessment by microbiological parameters in the Rybinsk Reservoir near Cherepovets, in Vliyanie stokov Cherepovetskogo promyshlennogo uzla na ekologicheskoe sostoyanie Rybinskogo vodokhranilishcha (Impact of Wastewater of the Cherepovets Industrial Unit on the Environmental State of the Rybinsk Reservoir), Rybinsk: Inst. Biol. Vnutr. Vod AN SSSR, 1990, pp. 24–41.
Spitsyna, T.P., Khokhlova, A.I., and Stepen’, R.A., A system for quantitative assessment of the degree of pollution of surface waters, Vestn. Krasnoyarsk. Gos. Univ., Ser. Estestv. Nauki, 2006, issue 5, pp. 120–126.
Sursyakova, V.V., Bondareva, L.G., Burmakina, G.V., and Rubailo, A.I., New approaches to identifying the sources of phenol entry to surface waters, Dokl. Akad. Nauk, 2011, vol. 441, no. 6, pp. 767–770.
Ugolev, A.M. and Kuz’mina, V.V., Pishchevaritel’nye protsessy i adaptatsii u ryb (Digestive Processes and Adaptation in Fish), St. Petersburg: Gidrometeoizdat, 1993.
Flerov, B.A., Ekologo-fiziologicheskie aspekty toksikologii presnovodnykh zhivotnykh (Ecological and Physiological Aspects of Toxicology of Freshwater Animals), Leningrad: Nauka, 1989.
Flerova (Nazarova), E.A. and Zabotkina, E.A., Toxic effect of sublethal concentrations of phenol and naphthalene on crucian carp mesonephros, Toksikol. Vestn., 2012, no. 4, pp. 49–51.
Ali, S.M., Sabac, S.Z., Fayez, M., et al., The influence of agro-industrial effluents on River Nile pollution, J. Adv. Res., 2011, vol. 2, pp. 850–895.
Anson, M., The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin, J. Gen. Phys., 1938, vol. 22, pp. 79–83.
Charan, A.A., Verma, O.P., Mathur, A., et al., Evaluation of changes in metabolic parameters and enzymes involved in metabolic pathways in Clarias batrachus after exposure to phenolic compounds, Asian J. Biomed. Pharm. Sci., 2013, vol. 3, pp. 60–67.
Clayton, G.D. and Clayton, F.E., Patty’s Industrial Hygiene and Toxicology, New York: John Wiley and Sons, 1994.
De Felice, F.G. and Ferreira, S.T., Novel neuroprotective, neuritogenic and anti-amyloidogenic properties of 2, 4-dinitrophenol: the gentle face of Janus, IUBMB Life, 2006, vol. 58, no. 4, pp. 185–191.
Dobbins, D.C., Thornton-Manning, J., Jones, D.D., and Federle, T.W., Mineralization potential for phenol in subsurface soils, J. Environ. Qual., 1987, vol. 16, no. 1, pp. 54–58.
Ford, M.D., Delaney, K.A., Ling, L.J. and Erickson, T., Clinical Toxicology, Philadelphia: W.B. Saunders Company, 2001.
Igbinosa, E.O., Odjadjare, E.E., Chigor, V.N., et al., Toxicological profile of chlorophenols and their derivatives in the environment: the public health perspective, Sci. World J. Hindawi Publ. Corpor., 2013, Article ID 460215, 11. http://dx.doi.org/10.1155/2013/460215
Lewis, S., Grimwood, M., and Comber, S., Proposed Environmental Quality Standards for Phenol in Water, Bristol: Environ. Agency Rio House Waterside Drive Aztec West Almondsbury, 1995.
Michalowicz, J., The occurrence of chlorophenols, chlorocatechols and chlorinated methoxyphenols in drinking water of the largest cities in Poland, Polish J. Environ. Stud., 2005, vol. 14, no. 3, pp. 327–333.
Michałowicz, J. and Duda, W., Phenols—sources and toxicity, Polish J. Environ. Stud., 2007, vol. 16, no. 3, pp. 347–362.
Mishra, A. and Poddar, A.N., Hematological changes in the Indian Murrel (Channa punctatus Bloch) in response to phenolic industrial wastes of the Bhilai Steel plant (Chhattisgarh, India), J. Res. Chem. Environ., 2011, vol. 1, no. 2, pp. 83–91.
Roche, H. and Boge, G., In vivo effects of phenolic compounds on blood parameters of a marine fish (Dicentrarchus labrax), Comp. Biochem. Physiol., 2000, vol. 125C, pp. 345–353.
Taysse, L., Troutaud, D., Khan, N.A., and Deschaux, P., Structure–activity relationship of phenolic compounds (phenol, pyrocatechol and hydroquinone) on natural lymphocytotoxicity of carp (Cyprinus carpio), Toxicology, 1995, vol. 98, pp. 207–214.
Zaki, M.S., Fawzi, O.M., and Shalaby, S.I., Phenol toxicity affecting hematological changes in cat fish, Life Sci. J., 2011, vol. 8, no. 2, pp. 244–248.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © V.V. Kuzmina, A.F. Tarleva, E.L. Gracheva, 2017, published in Biologiya Vnutrennykh Vod, 2017, No. 2, pp. 104–111.
Rights and permissions
About this article
Cite this article
Kuzmina, V.V., Tarleva, A.F. & Gracheva, E.L. Influence of various concentrations of phenol and its derivatives on the activity of fish intestinal peptidases. Inland Water Biol 10, 228–234 (2017). https://doi.org/10.1134/S1995082917020092
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1995082917020092